쪽의 핵형분석과 rRNA 유전자의 염색체상 위치
Karyotypic Analysis and Physical Mapping of rRNA Gene Loci in Persicaria tinctoria
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초록
Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ㎛ to 1.50 ㎛ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.
Abstract
Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ㎛ to 1.50 ㎛ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.
참고문헌 (22)
- Bae KH, "The Medicinal Plants of Korea", pp.85~85 (2000), Kyohaksa Publishing Co., Seoul.
- Choi HW, "Molecular cytogenetics in Panax ginseng and its related species", 16th Int. Chromosome Confer., pp.32~32 (2007)
- Chung ES, "Factors affecting cell growth in suspension cultures of Polygonum tinctorium Lour", Korean J. Breed, 26, pp.172~176 (1994)
- Darlington CD, "Chromosome Atlas of Flowering Plants", pp.73~73 (1955), The McMillan Co., New York.
- Hong SP, "A palynotaxonomic study of Korean Polygonaceae", Korean J. Plant Tax, 13, pp.63~76 (1983), \
- Jiang J, "Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research", Genome, 49, pp.1057~1068 (2006)
- Kang SS, "A flavonoid glycocide from the leaves of Polygonum sachalinense (II)", Arch. Pharm. Res, 5, pp.13~15 (1982)
- 김수영, "지모에서 McFISH를 이용한 rDNAs의 물리지도 작성", 한국약용작물학회지, 12(6), pp.515~518 (2004), 한국약용작물학회
- 이중구, "체세포분열과 감수분열 및 bicolor FISH를 이용한 섬시호의세포유전학적 분석", 한국약용작물학회지, 14(6), pp.354~359 (2006), 한국약용작물학회
- 방재욱, "황기류 식물 3종의 세포유전학적 분석", 한국약용작물학회지, 14(4), pp.250~254 (2006), 한국약용작물학회
- Kwak M, "Variation of nuclear ribosomal ITS sequences of Polygonum section Persicaria (Polygonaceae) in Korea", Korean J. Plant Tax, 36, pp.21~39 (2006)
- Koo DH, "Cytogenetic analyses of Angelica plants using Feulgen staining and multicolor fluorescence in situ hybridization", Korean J. Plant Biotech, 30, pp.123~127 (2003)
- Lapitan NLV, "Organization and evolution of higher plant genomes", Genome, 35, pp.171~181 (1992)
- Lee TB, "Illustrated Flora of Korea", pp.305~313 (1985), Hyangmoonsa Co., Seoul.
- Lee YN, ") Chromosome numbers of flowering plants in Korea", J. Kor. Res. Inst. Better Living, 8, pp.21~31 (1971)
- Levan A, "Nomenclature for centromeric position in chromosomes", Hereditas, 52, pp.201~220 (1964)
- Nahar KK, "Differential chromosome banding in Persicaria posumbu (Hamilton ex. D Don) H Gross (Polygonaceae)", Bangl. J. Bot, 27, pp.103~107 (1998)
- Park JH, "The Encyclopedia of Chinese Crude Drugs", pp.1~973 (2002), Shinilsangsa Publishing Co., Seoul.
- Park JH, "Medicinal Plants of Korea", pp.988~1017 (2004), Shinilsangsa Publishing Co., Seoul.
- Schwarzacher T, "In situ localization of parental genomes in a wide hybrid", Ann. Bot, 64, pp.315~4324 (1989)
- Suh Y, "A phylogenetic study of Polygonum sect. Tovara (polygonaceae) based on ITS sequences of nuclear ribosomal DNA", J. Plant Biol, 40, pp.47~52 (1997)
- Yasuda K, "Genetic diversity of vegetable water pepper (Persicaria hydropiper (L.) Spach) as revealed by RAPD markers", Breed. Sci, 55, pp.7~14 (2005)