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개시호(Bupleurum longeradiatum)의 핵형분석과 rDNAs의 Physical Mapping

Karyotype Analysis and Phusical Mapping of rDNAs in Bupleurum longeradiatum

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Abstract

Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescencein in situ hybridization (McFISH)A technique were carrie out in Bupleurum longeradiatum. Somatic metaphase chromosome number was 2n+12. Karyotype was composed of three pairs of metacentrics (NO. 3,4 and 6) and three pairs of submetacentrics (NO. 1, 2 and 5). The length of somatic prometaphase chromosomes ranges from 2.55 to 5.05㎛ with total length of 18.15㎛. In FISH experiment, one pair of 5S rDNA signals was detected on the pericentromeric region of chromosome 4 and one pair of 45S rDNA signals was detected on the telomeric region of chromosome 2.

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