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인삼재배지의 토양추출물이 종자 발아와 세포의 항산화효소 활성에 미치는 영향

Cell Viability and Antioxidant Enzyme Activity in the Cell of Ginseng (Panax ginseng C.A. Meyer) Treated with Soil Extracts

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One hundred-eighty extracts of soil collected from ginseng (Panax ginseng C.A. Meyer) fields were subjected to lettuce germination test, electrolyte leakage, cell viability and antioxidant enzyme activity test. Regardless of various cultivation periods, there was no significant difference in soil pH, the content of organic matter and available phosphate in ginseng fields. Based on lettuce seed germination test, six soil extracts showing inhibition of germination and/or seedling growth were selected for further study. Selected soil extracts markedly inhibited cell viability of ginseng cultured cells but leakage of electrolytes were not affected by the treatment. Enzyme activity of superoxide dimutase in ginseng cultured cells was not affected by the treatment with the soil extracts. However, those of peroxidase and catalase were significantly inhibited by the treatment with soil extracts which showed inhibition of lettuce seed germination and seedling growth.

Abstract

One hundred-eighty extracts of soil collected from ginseng (Panax ginseng C.A. Meyer) fields were subjected to lettuce germination test, electrolyte leakage, cell viability and antioxidant enzyme activity test. Regardless of various cultivation periods, there was no significant difference in soil pH, the content of organic matter and available phosphate in ginseng fields. Based on lettuce seed germination test, six soil extracts showing inhibition of germination and/or seedling growth were selected for further study. Selected soil extracts markedly inhibited cell viability of ginseng cultured cells but leakage of electrolytes were not affected by the treatment. Enzyme activity of superoxide dimutase in ginseng cultured cells was not affected by the treatment with the soil extracts. However, those of peroxidase and catalase were significantly inhibited by the treatment with soil extracts which showed inhibition of lettuce seed germination and seedling growth.

참고문헌 (15)

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  3. Alscher,R.G., "Antioxidant in Higher Plant", pp.1~174 (1993), CRC Press
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  5. Chon,S.U., "Phytotoxic effect of lettuce leaf extract on seedling growth of crops and weeds", Kor. J. Plant Res, 7, pp.69~76 (2004)
  6. 권순태, "Superoxide dismutase의 활성차이에 따른 식물세포의 paraquat에 대한 반응과 핵 DNA 손상 검정", 생명과학회지, 14(4), pp.614~619 (2004), 한국생명과학회
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  8. Lee, I.H., "Growth of Panax ginseng affected by the annual change in physico-chemical properties of ginseng cultivated soil", Kor. J. Ginseng Sci, 13, pp.84~91 (1989)
  9. Lee,C.Y., "An echochemical study on soil sickness", Kor. J. Ginseng Sci, 1, pp.51~58 (1976)
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  14. 인삼연작장해의 생물학적방제연구, "김홍진", 한국인삼연초연구소, 1~101 (1986)
  15. 연작지 토양환경연구, "오승환", 한국인삼연초연구소, 5~22 (1908)

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