Anti-Melanogenic Effects of Aceriphyllum rossii Leaf Ethanol Extract on Melanocytes
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초록
We evaluated the skin whitening effects of Aceriphyllum rossii leaf ethanol extract (ARLEE) in vitro using melan-a cells. The maximum permissible level for ARLEE application to melan-a cells was 50 μg/mL. In gross observation, ARLEE reduced melanin deposition and melanocyte dendricity in a dose-dependent manner. When compared to treatment with vehicle, ARLEE treatment (50 μg/mL) significantly decreased melanin contents by 33.0% (p<0.001), an even greater decrease than arbutin (positive control) treatment (27.2%). ARLEE treatment (50 μg/mL) significantly decreased intracellular tyrosinase activity by 48.8% (p<0.001), an even greater decrease than seen after arbutin treatment (36.0%). ARLEE treatment (50 μg/mL) significantly decreased tyrosinase protein and mRNA expression by 30.5% and 37.7% (p<0.01), respectively. These results suggest that ARLEE has better skin whitening activity than arbutin in melan-a cells, and this activity is likely mediated at the level of tyrosinase transcription. These results suggest that ARLEE could be used in functional cosmetic materials to prevent or alleviate skin pigmentation.
Abstract
We evaluated the skin whitening effects of Aceriphyllum rossii leaf ethanol extract (ARLEE) in vitro using melan-a cells. The maximum permissible level for ARLEE application to melan-a cells was 50 μg/mL. In gross observation, ARLEE reduced melanin deposition and melanocyte dendricity in a dose-dependent manner. When compared to treatment with vehicle, ARLEE treatment (50 μg/mL) significantly decreased melanin contents by 33.0% (p<0.001), an even greater decrease than arbutin (positive control) treatment (27.2%). ARLEE treatment (50 μg/mL) significantly decreased intracellular tyrosinase activity by 48.8% (p<0.001), an even greater decrease than seen after arbutin treatment (36.0%). ARLEE treatment (50 μg/mL) significantly decreased tyrosinase protein and mRNA expression by 30.5% and 37.7% (p<0.01), respectively. These results suggest that ARLEE has better skin whitening activity than arbutin in melan-a cells, and this activity is likely mediated at the level of tyrosinase transcription. These results suggest that ARLEE could be used in functional cosmetic materials to prevent or alleviate skin pigmentation.
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