Establishment of Cell Suspension Cultures and Plant Regeneration in White Dandelion (Taraxacum coreanum NAKAI.)
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In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4- dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), N6-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.
Abstract
In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4- dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), N6-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.
참고문헌 (20)
- Ahn, D.K., "Illustrated book of Korean medicinal herbs", pp.134~ (1998), Kyohak Publishing Co. Ltd., Korea
- Akashi, T., "Biosynthesis of triterpenoids in cultured cells, and regenerated and wild plant organs of Taraxacum officinale", Phytochemistry, 36, pp.303~308 (1994)
- Bisset, N.G., "Herbal drugs and phytopharmaceuticals: A Handbook for Practice on a Scientific Basis", pp.486~489 (1994), CRC Press, Boca Raton, Ann Arbor, London, Tokyo
- Duncan, D.B., "Multiple range and multiple F tests", Biometrics, 11, pp.1~42 (1955)
- Hall, R.D., "The initiation and maintenance of plant cell suspension cultures", Plant Tiss. Cult. Manual, A3, pp.1~21 (1991)
- Hernandez-Galicia, E., "Studies on hypoglycemic activity of Mexican medicinal plants", Proc. West Pharmacol. Soc, 45, pp.118~124 (2002)
- Kang, M.J., "Current trends of research and biological activities of dandelion", Food Industry Nutr, 6, pp.60~67 (2001)
- Koo, H.N., "Taraxacum offcinale induces cytotoxicity through TNF-α and IL-1α secretion in Hep G2 cells", Life Sci, 74, pp.1149~1157 (2003)
- 박현용, "흰민들레 잎조직으로 부터 기관형성을 통한 식물체 재생", 한국약용작물학회지, 15(1), pp.62~66 (2007), 한국약용작물학회
- 이미현, "Agrobacterium rhizogenes-mediated transformation of Taraxacum platycarpum and changes of morphological characters", PLANT CELL REPORTS, 22, pp.822~827, SPRINGER
- 이미현, "민들레(Taraxacum platycarpum) 유식물 절편으로부터 부정아 발생에 미치는 auxin과 cytokinin의 영향 및 식물체 재생", Journal of Plant Biotechnology, 29(2), pp.111~115 (2002), 한국식물생명공학회
- Leu, Y.L., "Chemical constituents from roots of Taraxacum formosanum", Chem. Pharm. Bull, 53(7), pp.853~855 (2005)
- 염옥희, "할미 꽃 (Pulsatilla koreana NAKAI) 식물 절편체로부터 부정아 유도에 미치는 cytokinin의 영향 및 식물체 재 분화", Journal of Plant Biotechnology, 36(2), pp.170~173 (2009), 한국식물생명공학회
- Murashige, T., "A revised medium for rapid growth and bio assays with tobacco tissue culture", Physiol. Plant, 15, pp.473~497 (1962)
- 유승헌, "Diversity of Endophytic Fungi Associated with Taraxacum coreanum and Their Antifungal Activity", Mycobiology, 34(4), pp.185~190 (2006), 한국균학회
- Schütz, K., "Taraxacum--A review on its phytochemical and pharmacological profile", J. Ethnopharmacol, 107, pp.313~323 (2006)
- Sun Yan-Lin, "Establishment of a novel plant regeneration system from suspensionderived callus in the halophytic Leymus chinensis (Trin.)", Journal of Plant Biotechnology, 37(2), pp.228~235 (2010), 한국식물생명공학회
- Sweeney, B., "Evidence-based systematic review of dandelion (Taraxacum officinale) by natural standard research collaboration", J. Herb. Pharmacother, 5(1), pp.79~93 (2005)
- Wolbis, M., "Polyphenolic compounds in Taraxacum officinale", Acta Pol. Pharm. Drug Res, 50, pp.153~158 (1993)
- Yang, D.S., "The constituents of Taraxacum hallaisanensis roots", Arch. Pharm. Res, 19(6), pp.507~513 (1996)