Direct Multiple Shooting Induction of Taraxacum
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초록
Plants of the genus Taraxacum are well-known as a traditional herbal remedy with a long history, and they have also been extensively used as food, chemicals and cosmetics. In this study, four Taraxacum species distributed in Korea (T. mongolicum, T. officinale, T. mongolicum variation and T. officinale variation) were utilized for an efficient method for direct multiple shooting induction and regeneration, using leaf blade, transition zone, petiole and root as explants in MS media with various hormone concentration and combination. MS medium containing IAA 0.2 mg/L and TDZ 1.0 mg/L showed the highest induction frequency of all the hormone combinations. Besides, the induction of T. mongolicum variation was most effective comparing with the other three species by the average induction frequency of four explants. While the induction effect of leaf blade explant was more obvious than the other three explants. This system exhibited a rapid propagation of shoots from the leaf blade explants and makes it convenient to make use of these Taraxacum species to develop their diverse applications in the future.
Abstract
Plants of the genus Taraxacum are well-known as a traditional herbal remedy with a long history, and they have also been extensively used as food, chemicals and cosmetics. In this study, four Taraxacum species distributed in Korea (T. mongolicum, T. officinale, T. mongolicum variation and T. officinale variation) were utilized for an efficient method for direct multiple shooting induction and regeneration, using leaf blade, transition zone, petiole and root as explants in MS media with various hormone concentration and combination. MS medium containing IAA 0.2 mg/L and TDZ 1.0 mg/L showed the highest induction frequency of all the hormone combinations. Besides, the induction of T. mongolicum variation was most effective comparing with the other three species by the average induction frequency of four explants. While the induction effect of leaf blade explant was more obvious than the other three explants. This system exhibited a rapid propagation of shoots from the leaf blade explants and makes it convenient to make use of these Taraxacum species to develop their diverse applications in the future.
참고문헌 (19)
- Booth, A, "Regeneration in root cuttings in T. officinale part 2: effects of exogenous hormones on root segments and root callus cultures", New Phytol., 73, pp.453~460 (1974)
- Bowes,B.G, "Preliminary observations on organogenesis in T. officinale tissue cultures", Protoplasma, 71, pp.197~202 (1970)
- Bowes,B.G, "The occurrence of shoot teratomata in tissue cultures of T. officinale", Planta, 100, pp.272~276 (1971)
- Burrows,S., "The triterpene group. Part IV. The triterpene alcohols of Taraxacum root", J. Chem Soc., 141, pp.2042~2047 (1938)
- Chen, H., "Establishment and optimization of the regeneration system for common dandelion (T. officinale Weber)", Chin. J. Biotechnol., 21, pp.244~249 (2005)
- Hoya,A., "Germination characteristics of native Japanese dandelion autopolyploids and their putative diploid parent species", J. Plant Res., 120, pp.139~147 (2007)
- Katrin, S., "Taraxacum—a review on its phytochemical and pharmacological profile", J. Ethnopharmacol., 107, pp.313~323 (2006)
- Lee. M.H., "Agrobacterium rhizogenes- mediated transformation of Taraxacum platycarpum and changes of morphological characters", Plant Cell Rep., 22, pp.821~827 (2004)
- Masaaki.S., "Two branches of the lupeol synthases gene in the molecular evolution of plant oxidosqualene cyclases", Eur. J. Biochem., 266, pp.302~307 (1999)
- Molinari. L., "Plant regeneration from callus of apomictic and sexual lines of Paspalum simplex and RFLP analysis of regenerated plants", Plant Cell Rep., 21, pp.1040~1046 (2003)
- Murashige, T., "Revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Power, F.B, "The constituents of Taraxacum root", J. Chem. Soc. Trans., 101, pp.2411~2429 (1912)
- Qian,Y, "Factors affecting shoot regeneration of hypocotyl of Brassica campestris at high frequency from in vitro culture", J. Cq. Coll. Educ., 19, pp.13~15 (2006)
- Rivera-Nunez,D, "Taraxacum vulgare (Lam.) schrank on T. officinale Weber", Incafo Madrid, 35~36 (1991)
- William,M., "Identifying and harvesting edible and medicinal plants in wild (and not-so-wild) places", Crop husbandry.; Plant Taxonomy and Geography, 127~144 (1994)
- Wu,T, "Study on the callus induction and regeneration of bambusa emeiensis ‘viridiflavus’", NJ Fores Univ., 22, pp.19~22 (2008)
- Xu,X.F., "TDZ: an efficacious plant growth regulator", Chin. Bull. Bot., 20, pp.227~237 (2003)
- Yang,D.S., "The constituents of Taraxacum hallaisanensis roots", Arch. Pharmacol. Res., 19, pp.507~513 (1996)
- Zobayed,S.M.A., "Micropropagation of potato: evaluation of closed, diffusive and forced ventilation on growth and tuberization", Ann. Bot., 87, pp.53~59 (2001)