항염증 및 항산화 활성 보유 유용 식물 탐색
Screening of Useful Plants with Anti-inflammatory and Antioxidant Activity
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초록
본 연구는 식물자원을 재료로 하여 in vitro 항염증 및 항산화 활성을 분석함으로써 기능성소재로의 활용 가능성이있는 유망 후보자원을 발굴하고자 수행하였다. 이를 위해식물추출물 38종을 대상으로 세포증식에 대한 영향, 염증관련지표(nitric oxide, TNF-α, IL-6, IκBα, iNOS, COX-2)및 항산화항목(DPPH 라디칼 소거능, LDL 산화저해능) 및총페놀 함량에 대한 효과를 탐색하고 결과로부터 8종의 시료를 1차로 선발하였다. 선발된 8종의 시료에 대해서는 다시 농도별로 세포증식에 대한 효과, iNOS, COX-2의 발현및 NF-κB 전사에 대한 영향, peroxynitrite, ROS, nitricoxide 생성에 대한 IC50 값을 분석하여 보다 가능성이 있는식물을 유망후보자원으로 선발하고자 하였다. 그 결과, 소사나무(가지), 등골나물(잎), 으름(꽃) 및 개모시풀(뿌리)은항염증 혹은 항산화활성이 우수하나 농도에 따라 세포독성을 나타내었으므로 활용에 주의가 필요해보였으며, 어저귀(잎)은 항염증활성과 항산화활성이 비교적 우수하고 실험된 처리농도에서 세포독성이 없어 향후 in vivo 활성 검정등 심화연구를 통해 그 활용 가능성을 검토할 필요가 있다고 사료되었다.
Abstract
This study was conducted to select some useful plants as functional material candidates. A total of 38 plants were preliminarily screened for the anti-inflammatory and antioxidant activities. The preliminarily selected 8 plants were further investigated to verify the in vitro inhibitory effect on inflammation and oxidative stress. Boehmeria platanifolia (root),Carpinus coreana (branch), and Eupatorium japonicum (leaf) inhibited the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-treated RAW 264.7 cells. Eupatorium japonicum (leaf) suppressed the expression of cyclooxygenase-2 (COX-2), whereas Boehmeria platanifolia (root) and Prunus yedoensis (branch) inhibited the transcription of nuclear factor-kappa B (NF-κB). Treatment with the extracts (2.5 ~ 20 μg/ml) of Abutilon theophrasti (leaf,flower/seed) and Hemistepta lyrata (stem) did not show toxicity on RAW 264.7 cell proliferation, but treatment with 2.5 μg/ml of Boehmeria platanifolia (root) exhibited cell toxicity. Carpinus coreana (branch) and Prunus yedoensis (branch)showed potent scavenging activities on peroxynitrite. Akebia quinata (flower), Carpinus coreana (branch), and Prunus yedoensis (branch) effectively inhibited reactive oxygen species (ROS). Abutilon theophrasti (leaf), Boehmeria platanifolia (root), Carpinus coreana (branch), and Eupatorium japonicum (leaf) exhibited strong inhibitory capacity with regard to nitric oxide (NO) production. The results suggested that Abutilon theophrasti (leaf) has in vitro anti-inflammatory and antioxidant activities, and that is a useful functional material candidate.
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