국내 자생 자원식물들의 항산화 활성 검색
Screening of Natural Herb Resources for Anti-oxidative Effects in Korea
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초록
자생식물의 항산화 활성 탐색을 위하여 140종의 식물을 80% ethanol로 추출하여 활성을 확인하였다. 그 결과 며느리배꼽이 가장 높은 항산화 활성을 나타내었고(VitaminE, ORACPE=1.0), 쉬땅나무, 찔레나무, 좁쌀풀, 갈참나무, 신갈나무 그리고 싸리의 순으로 항산화활성이 높은 것으로 조사되었다(ORACPE ≥ 1.50). 또한 80% 에탄올 추출물의 용매별(n-hexane, CH2Cl2, EtOAc, n-BuOH, water) 분획층 ORAC 결과에서도 며느리배꼽과 쉬땅나무가 각각 1.13 ± 0.002, 1.10 ± 0.004, 3.67 ± 0.232, 1.62 ± 0.049, 1.05 ± 0.003; 1.02 ± 0.007, 1.00 ± 0.016, 2.94 ± 0.130, 1.80 ± 0.019 그리고 1.04 ± 0.012로 높은 값을 나타내었다. 그 중 항산화 활성이 가장 높은 EtOAc 분획층의 유효성분 규명 등의 추가 연구가 필요하다.
Abstract
In the present study, the anti-oxidative properties of 80% ethanol extracts from 140 kinds of natural plants were investigated. Persicaria perfoliata had the highest anti-oxidative relative potency(VitaminE, ORACPE=1.0), followed by Sorbaria sorbifolia var. stellipila, Rosa multiflora, Lysimachia vulgaris var. davurica, Quercus aliena, Quercus mongolica and Lespedeza bicolor(ORACPE ≥ 1.50). Among them, the anti-oxidative activity values of the n-hexane, CH2Cl2, EtOAc, n-BuOH, and water fractions of the 80% EtOH extracts of P. perfoliata and S. sorbifolia in the ORAC assay system were 1.13 ± 0.002, 1.10 ± 0.004, 3.67 ± 0.232, 1.62 ± 0.049 and 1.05 ± 0.003; 1.02 ± 0.007, 1.00 ± 0.016, 2.94 ± 0.130, 1.80 ± 0.019 and 1.04 ± 0.012, respectively, with marked anti-oxidative activity in the ethyl acetate fraction from selected the 2 plant extracts.
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