경정배양에 의한 한국 자생 비비추속 식물의 기내증식
In vitro Multiplication of Hosta Tratt. Species Native to Korea by Shoot-tip Culture
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초록
본 연구는 우리나라에 자생하고 있는 비비추속 식물 6종(일월비비추, 주걱비비추, 다도해비비추, 좀비비추, 한라비비추,흑산도비비추)의 정단을 이용하여 대량증식과 품종개발 등을위한 기내증식체계를 확립하고자 하였다. 정단은 0.5, 1.0, 2.0,4.0 ㎎/L BA와 0.1, 0.5, 1.0, 2.0 ㎎/L TDZ를 0.1 ㎎/L NAA와각각 혼용한 조건과 PGRs을 무첨가한 조건(control)의 MS배지에 배양하였다. 배양 8주 후에 embryogenic callus, somaticembryo, crown bud, 그리고 신초와 뿌리의 분화 및 생육, 생체중등에 대하여 조사하였다. 6종의 비비추 식물 모두에서 control에비하여 PGRs 처리구의 embryogenic callus와 somatic embryo 형성율, 다신초 분화율이 높았다. 분화된 신초의 개수는 일월비비추는 2.0 ㎎/L TDZ에서 5.4개, 주걱비비추와 다도해비비추는 1.0 ㎎/L TDZ에서 각각 3.3개, 5.8개, 좀비비추는 0.5㎎/LBA에서 11.1개, 흑산도비비추는 0.5 ㎎/L TDZ에서 9.8개, 한라비비추는 1.0 ㎎/L BA, 0.1 ㎎/L TDZ에서 8.1개로 가장 많았다. Somatic embryo 형성에서는 다도해비비추와 흑산도비비추가처리한 PGRs에 대해 효과적이었고, 일월비비추, 주걱비비추,좀비비추, 한라비비추에서는 상대적으로 효과가 적었다. 4종의자생 비비추(일월비비추, 주걱비비추, 다도해비비추, 흑산도비비추)에서 조사된 crown bud도 control에 비하여 PGRs 처리구에서 더 많이 형성되었다. 주걱비비추는 cytokinin의 종류 및 농도와 상관없이 callus와 신초 분화에 큰 효과가 나타나지 않았지만, crown bud의 형성에는 TDZ에서 약간 증가하였다.
Abstract
The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta speciesnative to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of eachHosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 ㎎/L BA with 0.1 ㎎/L NAA,0.1, 0.5, 1.0, 2.0 ㎎/L TDZ with 0.1 ㎎/L NAA, and without any PGRs (control). They were investigated on callus, somaticembryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hostaspecies, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher thanthat of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/LTDZ in H. capitata (5.4), 1.0 ㎎/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 ㎎/L BA in H. minor(11.1), 1.0 ㎎/L BA and 0.1 ㎎/L TDZ in H. venusta (8.1), and 0.5 ㎎/L TDZ in H. yingeri (9.8). In somatic embryoformation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effectswere relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species(H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRstreatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiationregardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.
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