중이온 빔조사 담배(Nicotiana plumbaginifolia) 식물체의 생장과 DNA 변이
Growth and DNA Alteration of Heavy-ion Beam Irradiated Tobacco(Nicotiana plumbaginifolia) Plant
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참고문헌
초록
중이온빔(20Ne)을 담배 식물체에 조사(irradiation) 하여 식물체의 생장과 DNA의 변이에 미치는 영향을 검토하였다. 발아율과 초장은 조사선량이 증가함에 따라 감소하였다. 반면 5Gy~10Gy의 저선량에서 추대와 개화가 촉진되어 발육은 오히려 촉진되었다. 총 100개의 primer를 이용하여 RAPD 분석한 결과, 59개의 primer에서 총 336개의 DNA 단편이 증폭되었고, 1개의 primer에서 중이온빔 조사처리구에서만 출현하는 DNA 단편이 나타났다. AFLP분석 결과, 개체 특이적인 DNA 단편은 나타난 반면 중이온빔 처리구 특이적인 DNA 단편은 관찰되지 않았다.
Abstract
Effects of heavy-ion beam(20Ne) irradiation on growth and DNA alteration of tobaccoplants were investigated. Seed germination and plant height were decresed as the ion-beamintensity was increased. However, the bolting and flowering were promoted by the lowintensities of 5 Gy to 10 Gy treatment. Out of the 100 primers screened, 59 primersgenerated 336 DNA fragments by RAPD analysis, and one specific DNA fragment thatamplified in control but not in the ion-beam irradiated plants was observed. By AFLPanalysis, DNA fragment difference related to the ion-beam treatment was not detected butobserved among the plant bodys. .
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