Neutron 빔조사 담배 및 벼식물체의 특성

Abstract

Effects of neutron beam irradiation on seed germination, growth and RAPD pattern oftobacco (Nicotiana tabacumL. cv.; N. plumbaginifolia) and rice (Orya sativaL. cv.) plantswere estimated. Seed germination rate was not significantly changed by the neutron beamtreatment in both tobacco and rice seeds. And there was no significant differance in growthof the plants by the neutron beam treatment. Interestingly, however, some of morphologicalchanges, including leaf shape (about 36%), stem color and leaf color were observed inneutron beam treated tobacco plants. In addition, abnormal flower in petal was observed inthe neutron beam treated plant. This results indicate that neutron beam is able to use as aneffective mutagen in plant mutations. Scorable products from 20 primers were obtained byRAPD analysis in the leaves of the beam irradiated tobacco plants and most of the plantsshowed the similar band patterns.