Isolation of Differentially Expressed Genes by Low Temperature Treatment inWinter Oilseed Rape (Brassica napus L. cv. Tammi)

Abstract

To investigate flowering related genes in winter-type oilsed rape (Brassica napusL. cv. Tammi), diferentialy ex-a result of suppression subtractive hybridization (SSH), 288 clones were randomly selected from SSH library. Using reverseNorthern blot analysis, 150 of 28 clones were identified to be diferentialy expressed. Out of these 150 clones, 45 clones howedvery high identities with the known genes. Four clones howed very high identities over 90% with metallothionein-like gene thatis related to flowering-induced genes. Of these 4 clones, the cDNAclone,rfs-13, revealed high identity with metallothionein-likeproteininArabidopsisthaliana(98%)and (89%). Furthermore, geneexpressedinimatureflowerstageswasconfirmed by Northern blot analysis.Key words- Brassica napus, cDNA, Flowering, Oil seed rape, Suppression subtractive hybridization (SSH), Transcription