Isolation of Genes Related to Light and Low Temperature Stress in Barley (Hordeum vulgare L.)
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초록
To investigate low temperature- and light stress-induced genes of Hordeum vulgare L. cv. Dongbori #1, suppression subtractive hybridization (SSH) was performed with mRNAs from leaf samples that treated with low temperature (4oC) and extremely high light density (1500mmolm-2s-1). Ninety six clones were randomly selected from SSH library. Using dot blot analysis, 20 of 96 clones randomly selected were identified to be differentially expressed. Of these 20 clones, two showed homology to senescence-associated genes and one was similar to cytochrome P450 like-TBP, while the remaining 17 clones did not match to the known genes. Furthermore, a cDNA library was constructed using mRNA isolated from Hordeum vulgare L. cv. Dongbori #1 leaves treated with low temperature (4oC). cDNAs of 176 clones randomly selected from this library were isolated and sequenced. Database comparisons of cDNAs in GenBanks non-redundant databases using BLAST revealed that 129 of the 176 cDNAs (73.3%) showed a high degree of sequence homology to genes from other organisms. The energy category showed a considerably higher gene expression level (21.7%) than other categories, and genes of disease/defense category were also found at high frequencies (11.6%).
Abstract
To investigate low temperature- and light stress-induced genes of Hordeum vulgare L. cv. Dongbori #1, suppression subtractive hybridization (SSH) was performed with mRNAs from leaf samples that treated with low temperature (4oC) and extremely high light density (1500mmolm-2s-1). Ninety six clones were randomly selected from SSH library. Using dot blot analysis, 20 of 96 clones randomly selected were identified to be differentially expressed. Of these 20 clones, two showed homology to senescence-associated genes and one was similar to cytochrome P450 like-TBP, while the remaining 17 clones did not match to the known genes. Furthermore, a cDNA library was constructed using mRNA isolated from Hordeum vulgare L. cv. Dongbori #1 leaves treated with low temperature (4oC). cDNAs of 176 clones randomly selected from this library were isolated and sequenced. Database comparisons of cDNAs in GenBanks non-redundant databases using BLAST revealed that 129 of the 176 cDNAs (73.3%) showed a high degree of sequence homology to genes from other organisms. The energy category showed a considerably higher gene expression level (21.7%) than other categories, and genes of disease/defense category were also found at high frequencies (11.6%).
참고문헌 (15)
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