현탁배양을 통한 땃두릅나무의 체세포배 유도
Establishment of Suspension Culture System to Induce Somatic Embryo in Oplopanax elatus Nakai
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참고문헌
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초록
This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of NH4NO3 and KNO3 at the ratio of 1650 (㎎/ℓ ) : 1900 (㎎/ℓ ) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with 170 ㎎/ℓ KH2PO4. The addition of 1 and 3%sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.
Abstract
This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of NH4NO3 and KNO3 at the ratio of 1650 (㎎/ℓ ) : 1900 (㎎/ℓ ) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with 170 ㎎/ℓ KH2PO4. The addition of 1 and 3%sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.
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