조직배양에 의한 네리네 대량증식
Bulb Propagation on Nerine by Tissue Culture
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초록
Nerinefrom south africa and its sparkling flower shape make us estimate it as a hopeful kind of cut follow.There was a few studies on Nerinein korea. We started this study to set bulb propagation methods. The propagation bylot of Bulblet was NAA 00.5 + BA 0.52.0mgL-1 in Nerine bowdenii‘Favourite’and Nerine sarniens‘Red’respectively. The adjust culture media source for tissue culture were glucose 9% as a carbon source and (NH4+NO3)40mM as a nitrogen source. When glucose was used as a carbon source, Bulblet were harvested a little bit low thennerinetissue culture. When weconsist culture media as MS+BA 1.0mgL-1 + sucose 7% + (NH4+NO3) 40mM, the produced Bulblet were reached up to1.7 each per bulb and emergence rate was up to 100% irrespective of acclimatization period. The suitable culture explantfor nerinetissue culture was scale. When scale was cultured with MS+BA 1.0mgL-1+sucose 7%, its propagationefficiency was 54 times greater than using growing point. A proper culture part of the scaly leaf was middle part (8 scalyleaf from outer 8th scaly leaf) when middle part was cultured the number of Bulblet were up to 1.8 each per explant.
Abstract
Nerinefrom south africa and its sparkling flower shape make us estimate it as a hopeful kind of cut follow.There was a few studies on Nerinein korea. We started this study to set bulb propagation methods. The propagation bylot of Bulblet was NAA 00.5 + BA 0.52.0mgL-1 in Nerine bowdenii‘Favourite’and Nerine sarniens‘Red’respectively. The adjust culture media source for tissue culture were glucose 9% as a carbon source and (NH4+NO3)40mM as a nitrogen source. When glucose was used as a carbon source, Bulblet were harvested a little bit low thennerinetissue culture. When weconsist culture media as MS+BA 1.0mgL-1 + sucose 7% + (NH4+NO3) 40mM, the produced Bulblet were reached up to1.7 each per bulb and emergence rate was up to 100% irrespective of acclimatization period. The suitable culture explantfor nerinetissue culture was scale. When scale was cultured with MS+BA 1.0mgL-1+sucose 7%, its propagationefficiency was 54 times greater than using growing point. A proper culture part of the scaly leaf was middle part (8 scalyleaf from outer 8th scaly leaf) when middle part was cultured the number of Bulblet were up to 1.8 each per explant.
참고문헌 (13)
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