네리네 (Nerine bowdenii)의 기내 인편배양시 자구형성에 미치는 생장조절제와 Sucrose 농도의 영향
Effects of Growth Regulators and Sucrose Concentrations on the Bulblet Formation through In Vitro Culture of Scale Segment in Nerine bowdenii
0
조회
0
다운로드
1
인용
0
참고문헌
초록
네리네의 인편 배양에 의한 기내 증식을 위하여, 쌍인편으로부터 자구형성에 미치는 NAA와 BA 및 sucrose 농도의 영향을 조사하였다. 네리네의 쌍인편으로부터 기내 자구형성에 가장 적합한 배지는 1.0 mg/L NAA와 2.0 mg/L BA를 첨가한 MS배지였다. 적정 sucrose 농도는 30 g/L에서 가장 양호하였으며, 90 g/L 이상의 sucrose 첨가배지에서의 자구형성은 심하게 억제되었다. 비대된 인편을 단축경과 함께 5 mm 크기로 잘라 60일 간격으로 1, 2, 3차 계대배양한 결과, 절편당 자구수는 6.5, 7.3, 8.2개로 다수의 기내자구를 지속적으로 생산할 수 있었다. 기내 형성된 3 mm 이상의 자구는 생장조절제를 첨가하지 않은 MS배지에 옮겨 60일간 생육시킨 후, 초장 50 mm 이상 자란 식물체를 버미큐라이트와 펄라이트를 1:1 혼합한 배양토에 순화시켰을 때, 생존율은 95% 이상으로 높았다.
Abstract
The twin-scale segments of nerine (Nerine bowdenii) were cultured to investigate the influence of NAA, BA and sucrose concentrations on in vitro bulblet formation. The formation of bulblets from twin-scale segments showed a good response both the percentage of bulblet formation and the number of bulblets per explant on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. Formation of bulblet showed the highest efficiency on medium containing 30 g/L, and the formation of bulblets was strongly inhibited on medium containing over 90 g/L. When the twin-scale segments formed bulblets were subcultured three times to the same medium by 60 day subculture interval, the number of bulblets per explant was 6.5, 7.3 and 8.2 in order of first, second and third. The bulblets over 3 mm in diameter were hypertrophied and rooted after transferring to the hormone-free MS medium. The plantlets over 50 mm in height were successfully acclimatized in the soil mixed with the same volume of vermiculite and perlite, and the survival rate was over 95%.
참고문헌 (16)
- Chung HJ, "Regeneration of bulblets from bulblet derived bulb-scales of Lilium longiflorum", 22, pp.89~93 (1995)
- De Bruyn MH, "In vitro propagation of Amaryllis belladonna", 31, pp.179~184 (1992)
- Grootaarts H, "The origin of bulblets formed on excised twin scales of Nerine bowdeni", 1, pp.39~46 (1981)
- Han BH, "Effect of growth regulators on the bulblet formation through twin-scale segment culture in Hippeastrum hybridum Red Lion'", 18, pp.355~360 (1991)
- Han BH,, "Effect of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulbscales in Lilium oriental Hybrid Casa Blanca'.", 26, pp.103~107 (1999)
- Hussey G, "Totipotency in tissue explants and callus of some members of the Liliaceae", pp.253~262 (1975)
- Lee EM, "Effects of growth regulators on shoot differentiation and bulblet formation in shoot-tip and bulb-scale cultures of Lilium longiflorum", 22, pp.83~87 (1995)
- Lim S, "Effect of explant sources and plant growth regulators on bulblet formation in Lilium", 39, pp.111~114 (1998)
- Skoog F 1962, "A revised medium for rapid growth and bioassays with tobacco tissue culture", pp.473~497
- Niimi Y, "In vitro bulblet formation from leaf segment of lilies especially Lilium rubellum Barker", 11, pp.379~389 (1979)
- Slabbert MM, "Regeneration of bulblets from twin scales of Crinum macowanii in vitro", 33, pp.133~141 (1993)
- Stimart DP, "Tissue culture of bulb-scale sections for asexual propagation of Lilium longiflorum Thunb", 103, pp.182~184 (1978)
- Vishnevetsky J, "Carbohydrate metabolism in Nerine sarniensis bulbs developing in liquid culture", 108, pp.361~369 (2000)
- Vishnevetsky J, "Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro", 21, pp.645~650 (2003)
- Ziv M, "Scaled-up proliferation and regeneration of Nerine in liquid cultures Part Ⅰ The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors", pp.109~116 (1994)
- Ziv M, "Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro", 19, pp.845~850 (2000)