약용식물의 Peroxynitrite와 Hydroxyl radical 소거 활성
Peroxynitrite and Hydroxyl Radical Scavenging Activity of Medicinal Plants
2
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
The radical scavenging activities of 9 medicinal plants on peroxynitrite (ONOO-) and hydroxyl (∙OH) radical were investigated using in vitro system. The water extracts of 9 medicinal plants showed the protective effect against ONOO- and∙OH radical. In particular, Akebia quinata, Aster scaber, Cudrania tricuspidata, Diospyros kaki, Eriobotrya japonica, Lycium chinense, Parthenocissus tricuspidata and Polygonum aviculare exhibited ONOO-- scavenging activity by about 50% at the concentration of 10μg/ml. Although those ONOO--scavenging activities were lower than that of penicillamine (94.08±3.04%) as a positive control, Eriobotrya japonica (89.87±4.57%) was the most potent scavenger of ONOO- at the concentration of 10μg/ml. Also, Diospyros kaki and Urtica angustifolia showed the strong∙OH-scavenging activity than thiourea, positive control, at the concentration of lmg/ml. Our results indicate that 9medicinal plants may act as free radical scavengers and reduce damages caused by oxidative stress associated with ONOO- and∙OH radical.
Abstract
The radical scavenging activities of 9 medicinal plants on peroxynitrite (ONOO-) and hydroxyl (∙OH) radical were investigated using in vitro system. The water extracts of 9 medicinal plants showed the protective effect against ONOO- and∙OH radical. In particular, Akebia quinata, Aster scaber, Cudrania tricuspidata, Diospyros kaki, Eriobotrya japonica, Lycium chinense, Parthenocissus tricuspidata and Polygonum aviculare exhibited ONOO-- scavenging activity by about 50% at the concentration of 10μg/ml. Although those ONOO--scavenging activities were lower than that of penicillamine (94.08±3.04%) as a positive control, Eriobotrya japonica (89.87±4.57%) was the most potent scavenger of ONOO- at the concentration of 10μg/ml. Also, Diospyros kaki and Urtica angustifolia showed the strong∙OH-scavenging activity than thiourea, positive control, at the concentration of lmg/ml. Our results indicate that 9medicinal plants may act as free radical scavengers and reduce damages caused by oxidative stress associated with ONOO- and∙OH radical.
참고문헌 (36)
- Ames,B.N., "Uric acid provides an antioxidant defense in humans against oxidant-and radical-caused aging and cancer: a hypothesis", Proc. Natl. Acad. Sci. USA, 78, pp.6858~6862 (1981)
- Aruoma,O.I., "Antioxidant action of ergothioneine: assessment of its ability to scavenge peroxynitrite", Bioche. Biophys. Res. Commun, 231, pp.389~391 (1997)
- Beckman,J.S., "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxid", Proc. Natl. Acad. Sci. USA, 87, pp.1620~1624 (1990)
- Bramen,A.L., "Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene", J. Am. Oil Chem. Soc, 52, pp.59~63 (1975)
- Choi,J.S., "The structure-activity relationship of flavonoids as scavengers of peroxynitrite", Phytother. Res, 16, pp.232~235 (2002)
- Dalton,D.A., "Correlations between the ascorbate-glutathione pathway and effectiveness in legume root nodules", Physiol. Plants, 87, pp.365~370 (1993)
- Dreher,D., "Role of oxygen free radicals in cancer development", Eur. J. Cancer, 32A, pp.30~38 (1996)
- Fici.G.J., "Effects of lazaroids and a peroxynitrite scavenger in a cell model of peroxynitrite toxicity", Free Radic. Biol. Med, 22, pp.223~228 (1997)
- Fridorich,L., "The biology of oxygen radicals", Science, 201, pp.875~880 (1978)
- Griffiths,H.R., "The C1q binding activity of lgG is modified in vitro by reactive oxygen species: implications for rheumatoid arthritis", FEBS Lett, 388, pp.161~164 (1996)
- Haenen,G.R., "Peroxynitrite scavenging by flavonoids", Biochem. Biophys. Res. Commun, 236, pp.591~593 (1997)
- Halliwell,B, "DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems", FEBS Lett, 281, pp.9~19 (1991)
- Halliwell,B., "Free radicals in biology and medicine" (1999), Oxford University Press
- Song-HeeHan, "A New Phenolic Amide from Lycium chinense Miller", Archives of Pharmacal Research, 25(4), pp.433~437 (2002), 대한약학회
- Han,S.K., "Qualitative analysis of free radicals generated in AOPs by electron paramagnetic resonance (EPR)", J. of KSEE, 24, pp.1153~1161 (2002)
- Hatano,T., "Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols", Natural Medicines, 49, pp.357~363 (1995)
- Hsu,C.Y., "Antioxidant activity of extract from Polygonum aviculare L", Biol. Res, 39, pp.281~288 (2006)
- Huang,Y., "Anti-oxidative effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf in chronic bronchitis rats", Life Sci, 78, pp.2749~2757 (2006)
- Jung,H.J., "Structureactivity relationship of oleanane disaccharides isolated from Akebia quinata versus cytotoxicity against cancer cells and NO inhibition", Bio. Pharm. Bull, 27, pp.744~777 (2004)
- Katsube,T., "Screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay", J. Agric. Food Chem, 52, pp.2391~2396 (2004)
- Kim,S.Y., "Effect of heat treatment on the antioxidative and antigenotoxic activity of extracts from persimmon (Diospyros kaki L.) peel", Biosci. Biotechnol. Biochem, 70, pp.999~1002 (2006)
- Kooy,N.W., "Peroxynitrite-mediated oxidation of dihydrorhodamine 123", Free Radic. Biol. Med, 16, pp.149~156 (1994)
- Lee,B.W., "Selective ABTS radical-scavenging activity of prenylated flavonoids from Cudrania tricuspidata", Biosci. Biotechnol. Biochem, 70, pp.427~432 (2006)
- Lee,K.S., "Evaluation of the antioxidant potential of natural products", Combinatorial Chemistry & High Throughput Screening, 1, pp.35~46 (1998)
- Lin,K.T., "Reactive oxygen species participate in peroxynitrite-induced apoptosis in HL-60 cells", Biochem. Biophys. Res. Commun, 230, pp.115~119 (1997)
- Monsen,E.R., "Dietary reference intakes for the antioxidant nutrients : vitamin C, vitamin E, selenium, and carotenoids", J. AM. Diet. Assoc, 100, pp.637~640 (2000)
- Moure,A., "Natural antioxidant from residual sources", Food Chem, 72, pp.145~171 (2001)
- Namiki,M., "Antioxidants", antimutagens in food. Department of Brewing and Fermentation, 1990, pp.29, 273~300
- Patel,R.P., "Biological aspects of reactive nitrogen species", Biochim. Biophys. Acta, 1411, pp.385~400 (1999)
- Rice-Evans,C.A., "Structureantioxidant activity relationships of flavonoids and phenolic acids", Free Radic. Biol. Med, 20, pp.933~956 (1996)
- Rosen,G.M., "Spin trapping of free radicals during hepatic microsomal lipid peroxidation", Proc. Natl. Acad. Sci. USA, 78, pp.7346~7349 (1981)
- Rubbo,H., "Nitric oxide regulation of tissue free radical injury", Chem. Res. Toxicol, 9, pp.809~820 (1996)
- Muhammad Saleem, "Antioxidant Caffeic acid Derivatives from Leaves of Parthenocissus tricuspidata", Archives of Pharmacal Research, 27(3), pp.300~304 (2004), 대한약학회
- Soh,Y., "Protective effects of quinic acid derivatives on tetrahydropapaveroline-induced cell death in C6 glioma cells", Biol. Pharm. Bull, 26, pp.803~807 (2003)
- Squadrito,G.L., "Oxidative chemistry of nitric oxide: the roles of superoxide, peroxynitrite, and carbon dioxide", Free. Radic. Biol. Med, 25, pp.392~403 (1998)
- Virag,L., "Peroxynitrite induced cytotoxicity: mechanism and opportunities for intervention", Toxicol. Lett, 140-141, pp.113~124 (2003)