제초제 저항성 형질전환 벼 계통의 농업적 특성과 분자생물학적 평가
Evaluation of agronomic and molecular biological characteristics of the herbicide resistance transgenic rice
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Abstract
Agronomic characteristics of transgenic herbicide-resistant rice lines were evaluated under field condition. Differences in agronomic traits and rice quality were observed between transgenic plants and the corresponding untransformed controls. Transgenic Ilpumbyeo lines flowered earlier than untransformed controls, whereas transgenic Nagdongbyeo flowered at the same time as untransformed control. With regard to the yield and rice quality, most of the transgenic lines showed lower yield than control except some selected transgenic lines. Selected Nagdongbyeo transgenic line also showed good eating quality comparable to the control although most selected Ilpumbyeo transgenic lines showed slightly lower eating quality and increased white center/belly in the rice grain. In order to find the main factor leading to somaclonal variation among transgenic lines, Tos17, a rice retrotransposon activated by prolonged tissue culture period was investigated. Although the transgenic lines carried only one or two of transformed bar gene, the copy number of Tos17 increased in most transgenic lines compared with control. The activation of Tos17 was not detected in selected promising transgenic lines such as ND115-15-1-B and IP23-3-3, suggesting that the increased copy number of Tos17 may have negatively affected agronomic characteristics of transgenic rice.
참고문헌 (20)
- Bashir K, "Field evaluation and risk assessmentof transgenic indica basmati rice", 13, pp.301~312 (2004)
- Brar D, "Recovery of transgenic peanut", pp.745~753 (1994)
- Hiatt W, "Introduction and expression inplants of a glyfosate resistantaroAgene isolated fromSamonella typhimurium", pp.479~487 (1985)
- Sen L, "An alteredaroA gene product confers resistance to the herbicideglyphosate", pp.370~22 371 (1983)
- Datta S, "Herbicide resistant indica rice plants fromIRRI breeding 72 after PEG-mediated transformationof protoplasts", pp.619~629 (1992)
- Figueiredo L, "Transformation of potato", pp.666~670 (1994)
- Kiser J, "Efficienttransfer of a glyphosate tolerance gene into tomato usinga b i n a r yAgrobacterium tumefaciensvector", pp.726~730 (1987)
- Ohta S, "Eficienttransformation of rice mediated byAgro-bacteriumand sequence analysis of the boundaries ofthe T-DNA", pp.271~282 (1994)
- Hirochika H, "Retrotransposons of rice involved inmutations induced by tissue culture", 92, pp.8149~8153 (1996)
- James C, "Global status of commercialized biotech", GM crops, 2006a, ~
- James C, "GM crops : The first en years-globalsocio-economic and environmental impacts" (2006)
- Leung N, "Self-fertile transgenic wheat plants regeneratedfrom isolated scutella tissues following microprojectilebombardment with two distinct gene constructs", pp.285~297 (1994)
- Oard J.H, "Development, field evaluationand agronomic performanceof transgenic herbicideresistant rice", 2, pp.359~368 (1996)
- Powles S.B, "Herbicideresistance : impact and management", 58, pp.57~93 (1997)
- Fujimoto H, "Fertile transgenic rice plants regenerated from trans-formed protoplasts", pp.274~276 (1989)
- Tai T, "A rapid and inexpensivemethod for isolation of total DNA from dehydratedplant tissue", pp.297~303 (1990)
- Thompson C, "Characteri-zation of the herbicide-resistance gene bar fromStrepto-myces hygroscopicus Early infection of scutellum tissuewith Agrobacterium allows high-speed transformationof rice", pp.2516 1 969~2523 9 976
- Arimoto Y, "Transgenic plants after direct gene transfer intoprotoplasts", pp.1072~1074 (1988)
- Yamazaki M, "The rice retrotransposonTos17prefers low-copy-number sequences as integration targets", 265(2), pp.336~344 (2001)
- RDA, "Standard survey protocol for agriculturalexperiment", pp.490~510 (1995), RDA