SRAP을 이용한 국내육성 심비디움 품종의 유전적 다양성 분석
Analysis of Genetic Diversity in Cymbidium Varieties Using SRAP
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초록
Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between F_1 varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.
Abstract
Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between F_1 varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.
참고문헌 (17)
- Chang YK, "Analysis of genetic variability among Phalaenopsis species and hybrids using amplified fragment length polymorphism", J. Amer. Soc. Hort. Sci, 134(1), pp.58~66 (2009)
- Choi SH, "Genetic diversity and phylogenetic relationships among and within species of oriental cymbidiums based on RAPID analysis", SCIENTIA HORTICULTURAE, 108, pp.79~85 (2006), ELSEVIER SCIENCE BV
- Chung SY, "Genetic relationship and differentiation of Paphiopedilum and Phragmepedium based on RAPD analysis", SCIENTIA HORTICULTURAE, 109, pp.153~159 (2006), ELSEVIER SCIENCE BV
- Ferriol M, "Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers", Theor Appl Genet, 107, pp.271~282 (2003)
- Huang Y, "Analysis of diversity and relationships and among Chinese orchid cultivars using EST-SSR markers", Biochem. Syst. Ecol, 38, pp.93~102 (2010)
- 장성진, "한국 콩 보급품종을 포함한 엘리트품종의 SSR마커에 의한 유전적 다양성과 품종판별", 한국작물학회지, 54(2), pp.231~240 (2009), 한국작물학회
- Li G, "Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica", Theor Appl Genet, 103, pp.455~461 (2001)
- Lin Z, "Construction of a genetic linkage map for cotton based on SRAP", Chinese Science Bulletin, 48(19), pp.2064~2068 (2003)
- Murray MG, "Rapid isolation of high molecular weight plant DNA", Nucl. Acids Res, 8, pp.4321~4325 (1980)
- Obara-Okeyo P, "In vitro and in vivo characterization of cymbidium cultivars by isozyme analysis", J. Hort. Sci, 72(2), pp.263~270 (1997)
- Obara-Okeyo P, "Genetic diversity and identification of cymbidium cultivars as measured by random amplified polymorphic DNA (RAPD) markers", Euphytica, 99, pp.95~101 (1998)
- Sung Han OK, "Development and Utilization of Sequence Tagged Site-Polymerase Chain Reaction and Cleaved Amplified Polymorphic Sequence Markers for Classification of Oriental Cymbidium Cultivars and Variegates", Horticulture, Environment, and Biotechnology, 45(4), pp.216~222 (2004), 한국원예학회
- 박부희, "RAPD와 URP를 이용한 심비디움 유전자원 유연관계 분석", 화훼연구, 18(3), pp.201~206 (2010), 한국화훼학회
- Qiao LX, "Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers", Aquatic Botany, 87(3), pp.203~208 (2007)
- Ming-Mao Sun, "SSR 마커에 의한 최근 육성 보급된 한국 벼 품종의 다양성과 품종 판별", 한국육종학회지, 41(2), pp.115~125 (2009), 한국육종학회
- Young Hua Xu, "Genetic Diversity and Genetic Structures in Ginseng Landraces (Cultivars) by SRAP Analysis", 한국약용작물학회지, 18(3), pp.180~185 (2010), 한국약용작물학회
- Xue D, "The linkage maps Dendrobium species based on RAPD and SRAP markers", J. Genet. Genomics, 37, pp.197~204 (2010)