오죽 잎 용매분획물의 항산화 및 항균 활성
Antioxidant and antimicrobial activity of solvent fractions from black bamboo leaves
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
오죽 잎의 메탄올 조추출물을 극성에 따라 용매분획하고 분획물의 항산화 활성과 항산화 활성, S. aureus 균주에 대한 항균활성을 조사하고 활성성분을 HPLC를 이용하여 정량하였다. 용매분획물 중에서 ethyl acetate 분획물의 DPPH radical 소거 활성, FRAP활성이 모두 가장 강하였으며, 총페놀성 화합물의 함량도 가장 높았다. S. aureus 균주에 대한 항균활성 역시 ethyl acetate 분획물이 모든 시험 농도에서 가장 높은 활성을 보였다. Ethyl acetate 분획물 중에서 caffeic acid, ferulic acid, quercetin, kaempferol 성분을 분리하여 정량하였으며 이 중 kaempferol의 함량이 가장 높았다. 오죽 잎의 ethyl acetate 분획물은 천연 식품보존료로서 활용이 가능할 것으로 보인다.
Abstract
To investigate the natural antioxidant and antimicrobial phytochemicals from black bamboo (Phyllostachys nigra MUNRO) leaves, the solvent fractions from crude methanol extract were made with n-hexane, ethyl acetate, and butanol, and their antioxidant and antimicrobial activities were determined. The antioxidant activities were examined by 1,1-diphenyl-1-picrylhydrazyl (DPPH) method and ferric ion reducing antioxidant power (FRAP) method, and the antimicrobial activities against Staphylococcus aureus were tested by paper disc agar diffusion method. Total phenolic contents and total flavonoid contents of the solvent fractions were also determined. The ethyl acetate fraction with the highest total phenolic contents among all fractions showed the strong antioxidant activities by DPPH method and FRAP method, and antimicrobial activities against S. aureus at all test concentrations. Caffeic acid, ferulic acid, quercetin, and kaempferol were analyzed by HPLC in the ethyl acetate fraction from black bamboo leaves by the comparison with the standard chemicals. It is supposed that the ethyl acetate fraction from black bamboo leaves could be used as natural preservatives in the food industry.
참고문헌 (21)
- 백종원, "국내산 대나무 줄기와 잎의 에탄올 추출물의 항균활성", 한국식품과학회지, 34(6), pp.1073~1078 (2002), 한국식품과학회
- Sang Min Kim, "오죽잎차와 오죽죽순차의 성분 분석 및 항산화 효과", Journal of Applied Biological Chemistry, 55(2), pp.95~101 (2012), 한국응용생명화학회[DOI]
- 최선도, "초음파를 이용한 오죽으로부터 Flavone C-glycosides의 추출 및 항산화활성분석 향상", KSBB Journal, 23(4), pp.297~302 (2008), 한국생물공학회
- Shang YF, "Optimisation of pressurised liquid extraction of antioxidants from black bamboo leaves", Food Chem, 154, pp.164~170 (2014)
- 김연순, "추출 용매에 따른 오죽(Phyllostachys nigra Munro) 추출물의 생리활성 효과", 공업화학, 21(1), pp.6~10 (2010), 한국공업화학회
- Lee HJ, "The compound isolated from the leaves of Phyllostachys nigra protects oxidative stress- induced retinal ganglion cells death", Food Chem Toxicol, 48, pp.1721~1727 (2010)
- Blois MS, "Antioxidants determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Benzie IFF, "The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': The FRAP Assay", Anal Biochem, 239, pp.70~76 (1996)
- Singleton VL, "Analysis of total phenolic and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", Methods Enzymol, 299, pp.152~117 (1999)
- Jia Z, "The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
- Park SW, "Antimicrobial and antioxidative activities of solvent fraction from Humulus japonicus", Korean J Food Sci Technol, 26, pp.464~470 (1994)
- Kang SK, "Antimicrobial activity of defatted camellia (Camellia japonica L.) seed extract", J Korean Soc Food Sci Nutr, 27, pp.232~238 (1998)
- 장민우, "추출용매에 따른 브로콜리의 항산화와 항염증에 대한 효과", 한국식품위생안전성학회지, 27(4), pp.461~465 (2012), 한국식품위생안전성학회
- 강서진, "한국산 겨우살이 (Viscum album var. coloratum) 추출물의 식중독 세균 증식 억제 및 항산화 활성", 한국식품저장유통학회지, 19(6), pp.919~924 (2012), 한국식품저장유통학회
- Lee JH, "Some physiological activity of phenolic substances in plant foods", Korean J Food Sci Technol, 26, pp.317~323 (1994)
- Folin O, "On phosphotungastic phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~249 (1912)
- Proteggente AR, "The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin composition", Free Radical Res, 36, pp.217~233 (2002)
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
- 김선재, "약용식품 추출에 대한 항미생물 활성 검색과 폴리페놀 함량", 한국식품저장유통학회지, 11(2), pp.207~213 (2004), 한국식품저장유통학회
- Clark AM, "Antimicrobial activity of phenolic constituents of Mangnolia grandiflora L", J Pharm Sci, 70, pp.951~952 (1981)
- 김지영, "암대극(Euphorbia jolkini Boiss) 추출물의 항산화 및 항균활성", 한국식품과학회지, 38(5), pp.699~706 (2006), 한국식품과학회