Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants
0
조회
0
다운로드
16
인용
0
참고문헌
키워드
초록
The antioxidant potential and enzyme activities in Salicornia herbacea, Corylopsis coreana, Erythronium japonicum, Phragmites communis, Momordica Charantia, Nelumbo nucifera, Salvia plebeia, Portulaca oleracea, Ficus carica, Citrus junos and Cornus officinalis were determined. Their antioxidant activities were measured using DPPH radical scavenging and nitrite scavenging activity. Enzyme activities in investigated plants were evaluated as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The DPPH scavenging rate from 100 to 2500 mgL-1 was the highest in the flower of Corylopsis coreana. However, it was not detected in most of the samples at concentration below 100 mgL-1. The nitrite scavenging activity according to each kind of resource plants was significantly higher in the stem of Corylopsis coreana and leaf of Nelumbo nucifera.. The root extract of Erythronium japonicum had the highest SOD enzyme activity of 94.0% while leaf of Salvia plebeia showed the lowest SOD enzyme activity of 30.4%. The activity of CAT and APX showed higher values in the stem of Corylopsis coreana, root of Erythronium japonicum and root of Phragmites communis in comparison with other plants. The activity of POD showed significantly high values in stem of Corylopsis coreana, Momordica Charantia and pericarp of Citrus junos extracts. The antioxidant enzyme activities differ significantly in different plants. In conclusion, we showed that Corylopsis coreana,Erythronium japonicum Cornus officinalis, and Momordica Charantia had the potent biological activities. Therefore, these plant resources showing antioxidant activity could be good materials for development of source of functional healthy food.
Abstract
The antioxidant potential and enzyme activities in Salicornia herbacea, Corylopsis coreana, Erythronium japonicum, Phragmites communis, Momordica Charantia, Nelumbo nucifera, Salvia plebeia, Portulaca oleracea, Ficus carica, Citrus junos and Cornus officinalis were determined. Their antioxidant activities were measured using DPPH radical scavenging and nitrite scavenging activity. Enzyme activities in investigated plants were evaluated as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The DPPH scavenging rate from 100 to 2500 mgL-1 was the highest in the flower of Corylopsis coreana. However, it was not detected in most of the samples at concentration below 100 mgL-1. The nitrite scavenging activity according to each kind of resource plants was significantly higher in the stem of Corylopsis coreana and leaf of Nelumbo nucifera.. The root extract of Erythronium japonicum had the highest SOD enzyme activity of 94.0% while leaf of Salvia plebeia showed the lowest SOD enzyme activity of 30.4%. The activity of CAT and APX showed higher values in the stem of Corylopsis coreana, root of Erythronium japonicum and root of Phragmites communis in comparison with other plants. The activity of POD showed significantly high values in stem of Corylopsis coreana, Momordica Charantia and pericarp of Citrus junos extracts. The antioxidant enzyme activities differ significantly in different plants. In conclusion, we showed that Corylopsis coreana,Erythronium japonicum Cornus officinalis, and Momordica Charantia had the potent biological activities. Therefore, these plant resources showing antioxidant activity could be good materials for development of source of functional healthy food.
참고문헌 (34)
- Apel, K., "Reactive oxygen species: Metabolism, oxidative stress, and signal transduction", Annu. Rev. Plant Biol, 55, pp.373~399 (2004)
- Asada, K., "Production and action of active oxygen species in photosynthetic tissues, In Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants", pp.77~105 (1994), CRC Press
- Asish, K.P., "Salt tolerance and salinity effects on plants. A review", Ecotoxicol. Environ. Saf, 60, pp.324~349 (2005)
- Brand-Williams, W., "Use of a free radical method to evaluate antioxidant activity", Food Sci. Tech, 28, pp.25~30 (1995)
- 부희옥, "식물유래 천연색소의 항산화 활성", 한국자원식물학회지, 24(1), pp.105~112 (2011), 한국자원식물학회
- Chen, G.X., "Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties", Plant Cell Physiol, 30, pp.987~998 (1989)
- DuBok Choi, "Effect of bamboo oil on antioxidative activity and nitrite scavenging activity", Journal of Industrial and Engineering Chemistry, 14(6), pp.765~770 (2008), 한국공업화학회
- 천상욱, "서양민들레 부위별 추출물의 항산화 및 세포독성 연구", 한국자원식물학회지, 25(2), pp.232~239 (2012), 한국자원식물학회
- Cruz, C., "Uptake regions of inorganic nitrogen in roots of carob seedlings", Physiol. Plant, 95, pp.167~175 (1995)
- Dionisio-Sese, M.L., "Antioxidant responses of rice seedlings to salinity stress", Plant Sci, 135, pp.1~9 (1998)
- Egley, G.H., "Role of peroxidase in the development of water-impermeable seed coats in Sida spinos L", Planta, 157, pp.224~232 (1983)
- Foyer, C.H., "Protection against oxygen radicals: an important defence mechanism studied in transgenic plants", Plant Cell Env, 17, pp.507~523 (1994)
- Gaspar, T., "A two step control of basic and acid peroxidases and its significance for growth and development", Plant Physiol, 64, pp.418~423 (1985)
- 임무현, "솔잎발효추출물의 효소적 저해활성 및 아질산염 소거작용", 한국식품저장유통학회지, 11(1), pp.94~99 (2004), 한국식품저장유통학회
- Kandpal, R.P., "Alternation in the activities of the enzyme of proline metabolism in ragi (Eleusine coracane) leaves during water stress", J. Biosci, 3, pp.361~369 (1981)
- Kang, H.M., "Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid", Physiol. Plant, 115, pp.571~576 (2002)
- Kato, F.T., "Mutagenesis by carcinogenic nitroso compounds", J. Cell Physiol, 78, pp.139~144 (1971)
- Kato, H., "Inhibition of nitrosamine formation by nondialyzable melanoidins", Agric. Biol. Chem, 51, pp.1333~1338 (1987)
- Lazof, D.B., "Localization of nitrate absorption and translocation within morphological regions of the corn root", Plant Physiol, 100, pp.1251~1258 (1992)
- Lee, S.C., "Effect of far-infrared irradiation on catechins and nitrite scavenging activity of green tea", J. Agric. Food Chem, 54, pp.399~403 (2006)
- Mishra, N.P., "Changes in the activities of anti-oxidant enzymes during exposure of intact wheat leaves to strong visible light at different temperatures in the presence of protein synthesis inhibitors", Plant Physiol, 102, pp.903~910 (1993)
- Mittler, R., "Oxidative stress, antioxidants and stress tolerance", Trends Plant Sci, 7, pp.405~410 (2002)
- Moftah, A.H., "The effect of sodium chloride on solute potential and proline accumulation in soybean leaves", Plant Physiol, 83, pp.238~240 (1987)
- Najami, N., "Ascorbate peroxidase gene family in tomato: its identification and characterization", Mol. Genet. Genom, 279, pp.171~182 (2008)
- Oki, T, "Involvement of anthocyanins and other phenolic compounds in radical-scavenging activity of purple-fleshed sweet potato cultivars", Food Chem. Tox, 67, pp.1752~1756 (2002)
- Prakash, A., "Antioxidant activity, In Analytical Progress", pp.1~4 (2001), Medallion laboratories
- Rich, P.R., "The sites of superoxide anion generation in higher plant mitochondria", Arch. Biochem. Biophys, 188, pp.206~213 (1978)
- Saija, A.D., "‘In vitro’ evaluation of the antioxidant activity and biomembrane interaction of the plant phenols oleu-ropein and hydroxytyrosol", Int. J. Pharmacol, 166, pp.123~133 (1998)
- Sanchez, C.S., "Different radical scavenging tests in virgin olive oil and their relation to the total phenol content", Anal. Chemical Acta, 593, pp.103~107 (2007)
- 성낙주, "유자의 화학적 특성 및 아질산염 소거작용", 한국식품영양과학회지, 34(4), pp.496~502 (2005), 한국식품영양과학회
- Wheeler, G.L., "The biosynthetic pathway of vitamin C in higher plants", Nature, 393, pp.363~369 (1998)
- Wojtaszek, P., "Oxidative burst: an early plant response to pathogen infection", Biochem. J, 322, pp.681~692 (1997)
- Zeleneva, L.V., "Enzyme distribution of the cortex and the stele in cytodifferentiating maize seedlings", Biochem. Physiol. Pflanzen, 177, pp.97~106 (1982)
- Zhou, B.Y., "Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress", Crop Sci, 45, pp.599~605 (2005)