Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.
Abstract
Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.
참고문헌 (39)
- Bailey, J.P, "Chromosome number, morphology, pairing, and DNA values of species and hybrids in the genus Fallopia (Polygonaceae)", P. Syst. Evol, 180, pp.29~52 (1992)
- Bailey, J.P, "The potential role of polyploidy and hybridisation in the further evolution of the highly invasive Fallopia taxa in Europe", Ecol. Res, 22(6), pp.920~928 (2007)
- Baik, M.C, "A check-list of the Korean cultivated plants", Die Kulturpflanze, 34(1), pp.69~144 (1986)
- Batley, J, "high-throughput SNuPE assay for genotyping SNPs in the Flanking regions of Zea mays sequence tagged simple sequence repeats", Mol. Breeding, 11, pp.111~120 (2002)
- CBOL Plant Working Group, "A DNA barcode for land plants", PNAS, 106(31), pp.12794~12797 (2009)
- Chase, M.W, "Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid gene rbcL", Ann. Mo. Bot. Gard, 80(3), pp.528~580 (1993)
- Chase, M.W, "When in doubt, put it in Flacourtiaceae: a molecular phylogenetic analysis based on plastid rbcL DNA sequences", KEW Bulletin, 57(1), pp.141~181 (2002)
- Ching, A, "SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines", BMC Genet, 3(1), pp.19~ (2002)
- Chiang, T.Y, "Universal primers for amplification and sequencing a noncoding spacer between the atpB and rbcL genes of chloroplast DNA", Bot. Bull. Acad. Sin, 39, pp.245~250 (1998)
- 정준희, "The Effect of Pharmacopuncture with Polygonum cuspidatum Sieb et Zucc. Solution on Collagen-induced Arthritis in Wistar Rats", Korean Journal of Acupuncture, 28(1), pp.79~89 (2011), 경락경혈학회
- Conolly, A.P, "The distribution and history in the British Isles of some alien species of Polygonum and Reynoutria", Watsonia, 11, pp.291~311 (1977)
- Deynze, A.V, "Diversity in conserved genes in tomato", BMC Genet, 8, pp.465~ (2007)
- 도윤정, "하수오 물 추출물이 마우스 골다공증에 미치는 영향", 한국임상수의학회지, 28(4), pp.375~386 (2011), 한국임상수의학회
- Dorigo, W, "Mapping invasive Fallopia japonica by combined spectral, spatial, and temporal analysis of digital orthophotos", Int. J. Appl. Earth Obs. Geoinf, 19, pp.185~195 (2012)
- Doyle, J.J, "A rapid DNA isolation procedure for small quantities of fresh leaf tissue", Phytochem. Bull, 19, pp.11~15 (1987)
- Fay, M.F, "Familial relationships of Rhabdodendron (Rhabdodendraceae): plastid rbcL sequences indicate a caryophyllid placement", KEW Bulletin, 52(4), pp.923~932 (1997)
- Fourmann, M, "From Arabidopsis thaliana to Brassica napus: development of amplified consensus genetic markers (ACGM) for construction of a gene map", Theor. Appl. Genet, 105(8), pp.1196~1206 (2002)
- Gammon, M.A, "Haplotypes of Fallopia introduced into the US", Biol. Invasions, 12, pp.421~427 (2010)
- Hollingsworth, M.L, "Chloroplast DNA variation and hybridization between invasive populations of Japanese knotweed and giant knotweed (Fallopia, Polygonaceae)", Bot. J. Linn. Soc, 129(2), pp.139~154 (1999)
- Lahaye, R, "A test of psbK-psbI and atpF-atpH as potential plant DNA barcodes using the flora of the Kruger National Park as a model system (South Africa)", Nature Precedings (2008)
- Lajter, I, "Antiproliferative activity of Polygonaceae species from the Carpathian Basin against human cancer cell lines", Phytotherapy Research, 27(1), pp.77~85 (2013)
- Lee, W.T, "Distribution survey on medicinal plants in Kangwon province", Korean J. Plant. Res, 10(4), pp.275~291 (1997)
- Jun-Gyo In, "Molecular Identification of Korean Mountain Ginseng Using an Amplification Refractory Mutation System (ARMS)", Journal of Ginseng Research, 34(1), pp.41~46 (2010), 고려인삼학회
- Jaretzky, R, "Beiträge zur Systematik der Polygonaceae unter Berücksichtigung des Oxymethy lanthrachinon‐ Vorkommens", Repertorium Novarum Specierum Regni Vegetabilis, 22(4-12), pp.49~83 (1925)
- 김진모, "호장근의 일시적 국소뇌허혈 흰쥐 모델에 대한 신경보호효과", 대한본초학회지, 23(2), pp.225~233 (2008), 대한본초학회
- 김정성, "Molecular identification of Schisandra chinensis and its allied species using multiplex PCR based on SNPs", Genes & Genomics, 34(3), pp.283~290 (2012), 한국유전학회[DOI]
- Kim, J.Y, "Development and industrialization of functional bioactive material from the medicinal plan", J. Korean Soc. Food Sci. Nutr, 18(2), pp.1~6 (2013)
- Kim, J.Y, "Morphological and chromosomal variation in Fallopia section Reynoutria (Polygonaceae) in Korea", Brittonia, 52(1), pp.34~48 (2000)
- Kim, M.K, "Molecular authentication by multiplex-PCR of three similar medicinal plant species: Cynanchum wilfordii, Cynanchum auriculatum and Polygonum multiflorum (Fallopia multiflorum)", J. Med. Plants Res, 7(35), pp.2584~2589 (2013)
- Kim, S.B, "SNP Genotyping: Technologies and Biomedical Applications", Annu. Rev. Biomed. Eng, 9, pp.289~320 (2007)
- Kim, Y.W, "Current status of medicinal herbs recorded from the records in Gongju City, Chungcheongnam Province", Korea. Kor. Herb. Med. Inf, 1, pp.77~ (2013)
- 박진희, "한국산 닭의덩굴속 호장근절(마디풀과)의 화학분류학적 연구", 식물분류학회지, 41(1), pp.10~15 (2011), 한국식물분류학회
- Schuster, T.M, "Phylogenetic relationships of Muehlenbeckia, Fallopia, and Reynoutria (Polygonaceae) investigated with chloroplast and nuclear sequence data", Int. J. Plant Sci, 172(8), pp.1053~1066 (2011)
- Shaw, J, "Comparison of whole chloroplast genome sequences to shoose noncoding regions for phylogenetic studies in angiosperms: The tortoise and the hare Ⅲ", Am. J. Bot, 94(3), pp.275~288 (2007)
- 손호용, "Candidiosis 치료제 개발을 위한 약용 및 야생 식물의 항진균 활성의 검색", 생명과학회지, 13(5), pp.604~617 (2003), 한국생명과학회
- Steward, A.N, "The polygoneae of eastern Asia", Contr. Gray Herb, 5(88), pp.1~133 (1930)
- Stoll, P, "Response of plant and gastropod species to knotweed invasion", Basic Appl. Ecol, 13, pp.232~240 (2012)
- Taberlet, P, "Universal primers for amplification of tree non-coding regions of chloroplast DNA", Plant Mol. Biol, 17, pp.1105~1109 (1991)
- Yan, P, "Genetic variation and identification of cultivated Fallopia multiflora and its wild relative by using chloroplast matK and 18S rRNA gene sequences", Planta Med, 74, pp.1504~1509 (2008)
