한국산 둥굴레속 식물의 형태적 특성 및 엽록체 DNA 염기서열을 이용한 유연관계 분석
Morphological Characteristics and Phylogenetic Analysis of Polygonatum Species Based on Chloroplast DNA Sequences
2
조회
0
다운로드
8
인용
0
참고문헌
키워드
초록
Polygonatum is a genus placed in the family Liliaceae, distributed throughout the Northern Hemisphere and16 of the species are grown naturally in Korea. In oriental medicine, the rhizomes of Polygonatum have been used as two differentmedicines, Okjuk (Polygonati odorati Rhizoma) and Hwangjeong (Polygonati Rhizoma). However, it is difficult toidentify the morphological and chemical differences between the medicinal groups and thus easy to confuse the one with theother. Therefore, a clear classification standard needs to be established so as to be able to discriminate between them. In thestudy, the morphological characteristics of the plants, Polygonatum spp., were examined. Then, the differences in SNPsamong the DNA sequences of 7 of the Polygonatum spp. and 1 of the Disporum spp. were analyzed by DNA barcoding withrpoC1, rpoB2, matK, and psbA-trnH of the cpDNA region. In the results, three regions, rpoC1, rpoB2, and matK were usefulfor discriminating the species, P. stenophyllum and P. sibiricum. Furthermore, it was possible to discriminate the individualgermplasm within the species by using the combination of the results obtained from rpoB2, rpoC1, and matK.
Abstract
Polygonatum is a genus placed in the family Liliaceae, distributed throughout the Northern Hemisphere and16 of the species are grown naturally in Korea. In oriental medicine, the rhizomes of Polygonatum have been used as two differentmedicines, Okjuk (Polygonati odorati Rhizoma) and Hwangjeong (Polygonati Rhizoma). However, it is difficult toidentify the morphological and chemical differences between the medicinal groups and thus easy to confuse the one with theother. Therefore, a clear classification standard needs to be established so as to be able to discriminate between them. In thestudy, the morphological characteristics of the plants, Polygonatum spp., were examined. Then, the differences in SNPsamong the DNA sequences of 7 of the Polygonatum spp. and 1 of the Disporum spp. were analyzed by DNA barcoding withrpoC1, rpoB2, matK, and psbA-trnH of the cpDNA region. In the results, three regions, rpoC1, rpoB2, and matK were usefulfor discriminating the species, P. stenophyllum and P. sibiricum. Furthermore, it was possible to discriminate the individualgermplasm within the species by using the combination of the results obtained from rpoB2, rpoC1, and matK.
참고문헌 (20)
- Ahn DK., "A herbal study on Polygonatum species", Korean Journal of Pharmacognosy, 16, pp.105~113 (1985)
- 안희정, "안동지역 약용작물의 표고별 지표성분 분석", 한국약용작물학회지, 22(2), pp.91~97 (2014), 한국약용작물학회[DOI]
- 이미영, "RAMP를 이용한 황정과 위유의 유전적 분석", 한국약용작물학회지, 14(3), pp.125~129 (2006), 한국약용작물학회
- CBOL Plant Working Group., "DNA barcode for land plants", Proceedings of the National Academy of Sciences of the United States of America, 106, pp.12794~12797 (2009)
- Felsenstein J., "Confidence Limits on Phylogenies : An approach using the bootstrap", Society for the study of Evolution, 39, pp.783~791 (1985)
- Ha WY, "Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism(AFLP) and directed amplification of minisatellite region DNA(DAMD)", Journal of Agricultural and Food Chemistry, 27, pp.1871~1875 (2002)
- Hebert PDN, "Biological identifications through DNA barcodes", The Royal Society, 270, pp.313~321 (2003)
- Jang CG, "Taxonomic relationships of the Korean Polygonatum(Liliaceae)using the RAPDs analysis", Korean Journal of Plant Taxonomy, 28, pp.371~384 (1998)
- Jang CG., "A taxonomic review of Korean Polygonatum(Ruscaceae)", Korean Journal of Plant Taxonomy, 32, pp.417~447 (2002)
- 장계현, "한국 자생 둥굴레속 식물 지하경의 주요성분", 한국약용작물학회지, 10(3), pp.185~193 (2002), 한국약용작물학회
- Kim CM, "Encyclopedia of chinese herbal medicine(Vol. 10)", pp.6550~6556 (1998), Jungdammedia
- 이미영, "RAPD 분석에 의한 황정(黃精)과 위유(萎？)의 분류 검토", 생약학회지, 35(4), pp.265~270 (2004), 한국생약학회
- Li M, "Identification of herbal medicinal materials using DNA barcodes", Journal of Systematics and Evolution, 49, pp.271~283 (2011)
- Ma HL, "Species identification of the medicinal plant Tulipa edulis(Liliaceae)by DNA barcode marker", Biochemical Systematics and Ecology, 55, pp.362~368 (2014)
- 문병철, "AFLP 분석을 통한 Curcuma속 수집종간 유전적 다양성 분석", 한국약용작물학회지, 21(6), pp.455~460 (2013), 한국약용작물학회[DOI]
- Saitou N, "The neighbor-joining method : A new method for reconstructing phylogenetic trees", Molecular Biology and Evolution, 4, pp.406~425 (1987)
- 서용석, "玉竹의 지질강하 및 항산화효과", 한방재활의학과학회지, 21(2), pp.49~62 (2011), 한방재활의학과학회
- Tamura K, "Prospects for inferring very large phylogenies by using the neighbor-joining method", Proceedings of National Academy of Sciences of the United States of America, 101, pp.11030~11035 (2004)
- Williams JGK, "DNA polymorphism amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Research, 18, pp.6531~6535 (1990)
- 윤종선, "둥굴레속 식물의 18S rDNA 염기서열의 특성", 한국약용작물학회지, 14(3), pp.178~182 (2006), 한국약용작물학회