소리쟁이(Rumex crispus L.) 잎 추출물의 항산화 효과 및 각질세포 보호효과
Antioxidant effects of Rumex crispus L. leaf extracts and protective effects on Human HaCaT Keratinocyte
2
조회
0
다운로드
18
인용
0
참고문헌
키워드
초록
This study was carried out to investigate antioxidative effects of Rumex crispus L. (R. crispus) leaf extracts and protective effects on Human HaCaT keratinocyte. The extracts were prepared by ethanol extraction method. The antioxidant activities of extracts was used the method of DPPH radical, SOD similar activation, total polyphenolics and flavonoids content and protective effects on human HaCaT keratinocyte. The DPPH radical scavenging activity of the aerial part of R. crispus leaf extracts showed a considerable IC50 value (187.08 μg/ml) consistency showed an effect dependently. The contents of total phenolics (92.3 μg/ml) and flavonoids (173.1 μg/ml). The SOD-like activity of showed 39.26% at 500 ㎍/㎖. In our results, treatment with R. crispus leaf extracts did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 100 μg/ml. R. crispus leaf extracts showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly. These results suggested that R. crispus leaf extracts may have antioxidative effects on human skin and also potential as functional material and cosmetic products composites. So continuous studies for them would be desirable.
Abstract
This study was carried out to investigate antioxidative effects of Rumex crispus L. (R. crispus) leaf extracts and protective effects on Human HaCaT keratinocyte. The extracts were prepared by ethanol extraction method. The antioxidant activities of extracts was used the method of DPPH radical, SOD similar activation, total polyphenolics and flavonoids content and protective effects on human HaCaT keratinocyte. The DPPH radical scavenging activity of the aerial part of R. crispus leaf extracts showed a considerable IC50 value (187.08 μg/ml) consistency showed an effect dependently. The contents of total phenolics (92.3 μg/ml) and flavonoids (173.1 μg/ml). The SOD-like activity of showed 39.26% at 500 ㎍/㎖. In our results, treatment with R. crispus leaf extracts did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 100 μg/ml. R. crispus leaf extracts showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly. These results suggested that R. crispus leaf extracts may have antioxidative effects on human skin and also potential as functional material and cosmetic products composites. So continuous studies for them would be desirable.
참고문헌 (19)
- 최현숙, "오미자(Schizandra chinensis) 종자 추출물이 피부에 미치는 영향", 박사, pp.2009
- 김진화, "향나무 추출물의 광손상으로부터 피부세포 보호와 자극완화 효과에 대한 연구", 대한화장품학회지, 30(1), pp.63~71 (2004)
- 허성일, "땅빈대 추출물의 항산화 활성 및 세포독성 효과", 생약학회지, 39(4), pp.295~299 (2008), 한국생약학회
- 정은주, "뱀딸기 열매의 생리활성 성분과 화장품 응용에 관한 연구", 박사, pp.2008
- 홍정일, "냉이 에탄올 추출물의 유리라디칼 소거 및 항산화 활성", 한국식품영양학회지, 7(3), pp.169~176 (1994)
- 서훈석, "짚신나물, 삼백초의 항산화와 항암활성 효과", 한국약용작물학회지, 16(3), pp.139~143 (2008), 한국약용작물학회
- 서영준, "녹차 추출물의 산화적, 염증적 조직손상에 미치는 보호 효과", 국제녹차심포지엄, 통권 : 1, pp.103~118 (2003)
- 유지현, "솔잎 추출물의 항산화 효과", 생명과학회지, 14(5), pp.863~867 (2004), 한국생명과학회
- 이기동, "은행 종실 및 잎 추출물의 항산화 효과에 관하여", 경복대동학회지, 9, pp.61~69 (1991)
- Gunaydin, K., "dihydroxyanthraquinones and an anthrone from roots of Rumex crispus", Natural prpduct letters, 16(1), pp.65~70 (2002)
- 김영소, "천연 Quercitrin의 항허피스바이러스작용과 Nucleoside계 항허피스바이러스제와의 병용효과", 응용약물학회지, 7, pp.158~163 (1999)
- 배기환, "한국의 약용식물학", pp.92~ (2001), 서울: 교학사
- 이숙연, "소리쟁이 종자의 소염진통작용 및 간보호 효과", 생약학회지, 38(4), pp.334~338 (2007), 한국생약학회
- 박돈희, "소리쟁이 추출물의 항균 및 항산화 활성에 관한 연구", Korean Chemical Engineering Research(HWAHAK KONGHAK), 44(1), pp.81~86 (2006), 한국화학공학회
- Blois, M. S., "Antioxidant determi-nation by the use of a stable free radical", Nature, 26, pp.1198~1120 (1958)
- Swain, T., "Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents", Science of Food Agriculture, 10, pp.83~88 (1959)
- Davidson, P. M., "Method for testing the efficacy of food antimicrobia l s", Food technol, 43, pp.148~155 (1989)
- Marklund, S., "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur. J. Biochem, 47, pp.469~474 (1974)
- O'Toole, E. A., "Hydrogen peroxide inhibits human keratinocyte migration", Dermatol Surg, 22(6), pp.525~529 (1996)