작약 레드참 꽃잎의 이온화원-푸리에 변환 질량분석과 기능성 연구
Fourier Transform Ion Cyclotron Resonance (FT-ICR) MASS Spectrophotometric Analysis of Flower Petal from Paeonia lactiflora cv. ‘Red Charm’ and Evaluation of its Functional Activity
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본 연구에서는 작약 꽃잎의 기능성에 대한 분석 연구를 수행하였다. 이온화원-푸리에 변환 질량분석을 통해, 작약 레드참꽃잎의 70% 에탄올 추출액에서 쿼세틴 글루코파이러노사이드등을 비롯해 24개의 다른 성분을 발견하였다. DPPH 항산화 테스트 결과 0.1mg/ml 추출액 농도에서 87.9-90.4% 활성도를 보였다. 타이로시네이즈 미백 테스트에서는 0.1mg/ml 농도에서약 추출액은 알부틴과 비슷한 미백 효능을 보였다. 하이어루로니데이즈 항염 테스트에서는 추추출액 5% 농도에서 76.5% 저해도를 보였다. 결론적으로 본연구는 작약 꽃잎 추출액이 새로운 미백제로 개발될 가능성을 제시하였다.
Abstract
Little attention has been paid to the functional aspect of the flower petal of Paeonia lactiflora, compared to that of its root. To determine the components of flower petal of Paeonia lactiflora, we conducted the Fourier transform ion cyclotron resonance (FT-ICR) MASS spectrophotometric analysis. We detected the 24 different types of ingredients from the 70% ethanol extracts of flower petal of peonia lactiflora cv. ‘Red Charm’. The main compounds were quercetin glucopyranosides, methyl gallate, paonioflolol and kaemperol glucopyranosides. We further tested its functional activity. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of the extracts was 87.9-90.4% at 0.1mg/ml. This result showed that these flower extracts have approximately 5-fold stronger antioxidant potential than a previous report with root extracts (Bang et al. 1999). The result of tyrosinase inhibition assay of Paeonia lactflora extract was almost similar to that of arbutin except significantly higher effect in the coral sunset extract at 0.1% concentration. Hyaluronidase inhibition assay showed 76.5% inhibition at 5% concentration of this flower extract, indicating that Peaonia lactiflora flower extracts have the major anti-inflammatory, anti-oxidant and brightening effects. Taken together, these results suggest these three Paeonia lactiflora species extracts might provide the basis to develop a new natural brightening agent. Key words - Fourier transform-ion cyclotron resonance (FT-ICR) MASS, Paeonia lactiflora cv. ‘Red Charm’, Anti-oxidant activity, Tyrosinase inhibition assay, Hyaluronidase inhibition assay
참고문헌 (26)
- Bang, M. H., "Isolation and structure determination of antioxidants from the root of Paeonia lactiflora", J. Korean Soc. Agric. Chem. Biotechnol, 42(2), pp.170~175 (1999)
- Carmichael, J., "Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing", Cancer Res, 47(4), pp.936~942 (1987)
- Cheng, J. T., "Paeoniflorin reverses guanethidine-induced hypotension via activation of central adenosine A1 receptors in Wistar rats", Clin. Exp. Pharmacol. Physiol, 26, pp.815~816 (1999)
- Ghosh, P., "The role of hyaluronic acid(hyaluronan)in health and disease : interactions with cells, cartilage and components of synovial fluid", Clin. Exp. Rheumatol, 12(1), pp.75~82 (1994)
- Goulandris, N., "The ponies of Greece", House & Garden, 156, pp.252~256 (1984)
- Hwang, J. S., "Isolation and identification of antimicrobial compound from Jakyak (Paeonia japonica var. pilosa Nakai)", Korean J. Soc. Food Sci, 16(5), pp.445~446 (2000)
- 정혜란, "충남 내륙지역 민속식물의 전통지식", 한국자원식물학회지, 27(4), pp.309~325 (2014), 한국자원식물학회[DOI]
- 정연옥, "작약꽃을 이용한 미백 및 항산화 효과 분석", 동의생리병리학회지, 24(3), pp.452~456 (2010), 한의병리학회
- Kim, H. H., "Augmentation of UV-induced skin wrinkling by infrared irradiation in hairless mice", Mechanism of Ageing and Development, 126(11), pp.1170~1177 (2005)
- 김재철, "작약 재배년한 단축을 위한 효과적인 재배법", 한국자원식물학회지, 19(1), pp.83~11 (2006), 한국자원식물학회
- Kim, S. J., "Effect of irrigation treatment on growth and yield of Paeonia lactiflora Pallas L. (Peony)", Korean J. Plant Res, 14(1), pp.48~52 (2001)
- Kim, S. J., "Effects of vinyl mulching on growth and quality of peony(Paeonia lactiflora P. )RDA", J. Indus. Crop Sci, 40(1), pp.23~28 (1998)
- 권미화, "백작약 열수추출물의 피부노화 억제 효과 ( II ) -주름형성 억제 효과를 중심으로-", 환경독성보건학회지, 24(2), pp.169~178 (2009), 환경독성보건학회
- 이병근, "화장품 개발을 위한 배롱나무(Lagerstroemia indica Linnaeus) 가지 추출물의 생리활성에 관한 연구", 한국자원식물학회지, 27(1), pp.29~34 (2014), 한국자원식물학회[DOI]
- 이미경, "주요 식용꽃 추출물의 폴리페놀과 카테킨류 함량이 항산화 활성에 미치는 영향", 한국자원식물학회지, 27(2), pp.111~118 (2014), 한국자원식물학회[DOI]
- Lee, N. H., "Screening of the tyrosinase inhibition activities, and radical scavenging effects using plants in Cheju", Kor J. Pharmacogn, 32(3), pp.175~180 (2001)
- 이진영, "도화(桃花, Pruni persicae Flos) 분획물의 항산화 및 항염증 효과", 대한본초학회지, 27(5), pp.55~63 (2012), 대한본초학회
- Mlcek, J., "Quercetin and its anti-allergic immune response. Molecules 21(5)", Molecules, 21(5), pp.E623~ (2016)
- Nishida, S., "Induction of apoptosis in HL-60 cells treated with medicinal herbs", Amer. J. Chin. Med, 31, pp.551~562 (2003)
- 박현숙, "작약 추출물의 구강병원균에 대한 항균성 및 구강암 세포 증식 억제효과", 한국환경보건학회지, 33(1), pp.21~29 (2007), 한국환경보건학회
- 박문수, "전라남도 지역의 민속식물 이용현황", 한국자원식물학회지, 28(1), pp.79~92 (2015), 한국자원식물학회[DOI]
- Prieto, J. M., "Influence of traditional Chinese antiinflammatory medicinal plants on leukocyte and platelet functions", J. Pharm. Pharmacol, 55(9), pp.1275~1282 (2003)
- Reissig, J. L., "A modified colorimetric method for the estimation of N-acetylamino sugars", J. Biol. Chem, 217(2), pp.959~966 (1995)
- Sharma, O. P., "DPPH antioxidant assay revisited", Food Chem, 113, pp.1201~1205 (2009)
- Soka, T., "Encyclopedia of Chinese Medicine", Tokyo, Japan, 3, pp.2066~2070 (1985)
- Suh., "Screening of tyrosinase inhibitors from oriental herbs", Korean J. Plant Res, 14(1), pp.32~37 (2001)