Anti-inflammatory and Anti-Oxidant Effects of Oxypaeoniflorin, Paeoniflorin and Paeonia lactiflora cv. ‘Red Charm’ Flower Petal Extracts in Macrophage Cells
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The root extracts of Paeonia lactiflora cv. ‘Red Charm’ has been studied by many groups, however, littleattention has been paid to its flower petal. Paeonia is the genus in the Paeoniaceae family. ‘Red Charm’ Paeonia is asoft-stemmed herbaceous peony hybrid of P. officinalis and P. lactiflora. We previously showed the flower petal extract ofRed Charm might have anti-oxidant and anti-inflammatory activities, however, it was not clear which components might beinvolved in this activity. Bioinformatics analysis previously indicated these extracts have potential anti-oxidant materials. One of them is identified as paeoniflorin, which is major component in root extract of Red Charm. In this study, wecompared paeoniflorin and oxypaeoniflorin using DPPH assays to measure its anti-oxidant activities. Oxypaeoniflorinshowed higher levels of radical scavenging activity, similar to ascorbic acid control, whereas paeoniflorin did not. Furthermore, nitric oxide assay showed they have similar anti-inflammatory effects. Taken together, these results suggestoxypaeoniflorin may play a more important role in the anti-oxidant activity of the flower petal and root extracts of RedCharm, compared to paeoniflorin. Further studies may be able to provide a platform to develop potential dual effectstherapeutics for oxidant-mediated and inflammation-mediated disease in the near future.
Abstract
The root extracts of Paeonia lactiflora cv. ‘Red Charm’ has been studied by many groups, however, littleattention has been paid to its flower petal. Paeonia is the genus in the Paeoniaceae family. ‘Red Charm’ Paeonia is asoft-stemmed herbaceous peony hybrid of P. officinalis and P. lactiflora. We previously showed the flower petal extract ofRed Charm might have anti-oxidant and anti-inflammatory activities, however, it was not clear which components might beinvolved in this activity. Bioinformatics analysis previously indicated these extracts have potential anti-oxidant materials. One of them is identified as paeoniflorin, which is major component in root extract of Red Charm. In this study, wecompared paeoniflorin and oxypaeoniflorin using DPPH assays to measure its anti-oxidant activities. Oxypaeoniflorinshowed higher levels of radical scavenging activity, similar to ascorbic acid control, whereas paeoniflorin did not. Furthermore, nitric oxide assay showed they have similar anti-inflammatory effects. Taken together, these results suggestoxypaeoniflorin may play a more important role in the anti-oxidant activity of the flower petal and root extracts of RedCharm, compared to paeoniflorin. Further studies may be able to provide a platform to develop potential dual effectstherapeutics for oxidant-mediated and inflammation-mediated disease in the near future.
참고문헌 (35)
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