Anemarrhena asphodeloides Extract Inhibits the Mycelial Growth of Magnaporthe oryzae and Controls the Rice Blast Disease
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Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a newenvironment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenicfungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitoryeffects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showedprominent inhibitory effect of which IC50 was 139.7 ㎍/㎖. Mycelial radii of M. oryzae were measured on PDA mediumcontaining the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fractionshowed the impressive inhibitory effect of IC50, 54.12 ㎍/㎖. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almostequivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, theanalysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for theverification of cellular mechanism by which the mycelial growth of M. oryzae inhibited.
Abstract
Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a newenvironment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenicfungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitoryeffects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showedprominent inhibitory effect of which IC50 was 139.7 ㎍/㎖. Mycelial radii of M. oryzae were measured on PDA mediumcontaining the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fractionshowed the impressive inhibitory effect of IC50, 54.12 ㎍/㎖. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almostequivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, theanalysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for theverification of cellular mechanism by which the mycelial growth of M. oryzae inhibited.
참고문헌 (24)
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