GC-MS-based Metabolomics Reveals the Metabolic Profile of Cordyceps Militaris Mycelial Stimulated on Light Emitting Diode
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Cordyceps militaris (C. militaris) is a unique and valuable medicinal fungus belonging to the Cordyceps species. C. militaris is theonly fungus that contains cordycepin which is a biologically active compound. In previous studies, light-emitting diodes (LEDs) areknown to be effective in increasing cordycepin content, but metabolic profiling of LED-stimulated C. militaris has not been confirmed. Metabolic profiling is essential to understanding the metabolic regulation of cordycepin. This study studied the physiologically activesecondary metabolites of C. militaris according to the presence or absence of stimulation of LEDs through GC-MS analysis. Most ofthe metabolites were detected in both samples, but there was a clear difference in the detected concentration. In particular, C. militarishad a significant difference in amino acid levels when stimulated with LEDs. Our results suggested that LEDs could stimulate aminoacid synthesis in C. militaris mycelium to increase the cordycepin content.
Abstract
Cordyceps militaris (C. militaris) is a unique and valuable medicinal fungus belonging to the Cordyceps species. C. militaris is theonly fungus that contains cordycepin which is a biologically active compound. In previous studies, light-emitting diodes (LEDs) areknown to be effective in increasing cordycepin content, but metabolic profiling of LED-stimulated C. militaris has not been confirmed. Metabolic profiling is essential to understanding the metabolic regulation of cordycepin. This study studied the physiologically activesecondary metabolites of C. militaris according to the presence or absence of stimulation of LEDs through GC-MS analysis. Most ofthe metabolites were detected in both samples, but there was a clear difference in the detected concentration. In particular, C. militarishad a significant difference in amino acid levels when stimulated with LEDs. Our results suggested that LEDs could stimulate aminoacid synthesis in C. militaris mycelium to increase the cordycepin content.
참고문헌 (30)
- Adnan M, "Effect of pH, temperature and incubationtime on cordycepin production from Cordyceps militaris using solid-state fermentation on various substrates", CYTA J. Food, 15, pp.617~621 (2017)
- Blum BC, "Single-platform ‘multi-omic’ profiling : Unified mass spectrometry and computational workflows for integrative proteomics-metabolomics analysis", Mol. Omics, 14, pp.307~319 (2018)
- Chiang SS, "Effect of light-emitting diodes on the production of cordycepin, mannitol and adenosine in solid-state fermented rice by Cordyceps militaris", J. Food Compost. Anal, 60, pp.51~56 (2017)
- Da Fontoura Prates D, "Role of light emitting diode (LED) wavelengths on increase of protein productivity and free amino acid profile of Spirulina sp. cultures", Bioresour Technol, 306, pp.123184~ (2020)
- Das SK, "A new approach for improving cordycepin productivity in surface liquid culture of Cordyceps militaris using high-energy ion beam irradiation", Lett. Appl. Microbiol, 47, pp.534~538 (2008)
- Das SK, "Medicinal uses of the mushroom Cordyceps militaris : Current state and prospects", Fitoterapia, 81, pp.961~968 (2010)
- Dong JZ, "Light wavelengths regulate growth and active components of Cordyceps militaris fruit bodies", J. Food Biochem, 37, pp.578~584 (2013)
- Dong JZ, "Effects of selenium and light wavelengths on liquid culture of Cordyceps militaris link", Appl. Biochem. Biotechnol, 166, pp.2030~2036 (2012)
- 하시영, "Effect of Light-Emitting Diodes on Cordycepin Production in Submerged Culture of Paecilomyces japonica", 목재공학, 48(4), pp.548~561 (2020), 한국목재공학회
- Hyun SH, "Metabolic profiles and free radical scavenging activity of Cordyceps bassiana fruiting bodies according to developmental stage", PLoS One, 8, pp.e73065~ (2013)
- Idnurm A, "Light controls growth and development via a conserved pathway in the fungal kingdom", PLoS Biol, 3, pp.e95~ (2005)
- Kang C, "Optimization of large-scale culture conditions for the production of cordycepin with Cordyceps militaris by liquid static culture", The Scientific World J, pp.1~15 (2014)
- 강나루, "Development of High Cordycepin-Producing Cordyceps militaris Strains", Mycobiology, 45(1), pp.31~38 (2017), 한국균학회
- Khan MA, "Cordycepin in anticancer research : Molecular mechanism of therapeutic effects", Curr. Med. Chem, 27, pp.983~996 (2020)
- Lee HH, "Anti-cancer effect of Cordyceps militaris in human colorectal carcinoma RKO cells via cell cycle arrest and mitochondrial apoptosis", Daru, 23, pp.1~8 (2015)
- Leung PH, "Effects of ammonium feeding on the production of bioactive metabolites (cordycepin and exopolysaccharides) in mycelial culture of a Cordyceps sinensis fungus", J. Appl. Microbiol, 103, pp.1942~1949 (2007)
- Li LQ, "Anti-inflammation activity of exopolysaccharides produced by a medicinal fungus Cordyceps sinensis Cs-HK1 in cell and animal models", Int J. Biol. Macromol, 149, pp.1042~1050 (2020)
- Lin LT, "Optimal conditions for cordycepin production in surface liquid-cultured Cordyceps militaris treated with porcine liver extracts for suppression of oral cancer", J. Food Drug Anal, 26, pp.135~144 (2018)
- Masuda M, "Enhanced production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris", Enzyme Microb. Technol, 40, pp.1199~1205 (2007)
- 오준상, "Metabolomic profiling reveals enrichment of cordycepin in senescence process of Cordyceps militaris fruit bodies", The Journal of Microbiology, 57(1), pp.54~63 (2019), 한국미생물학회[DOI]
- Roux A, "Impact of collection conditions on the metabolite content of human urine samples as analyzed by liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectroscopy", Metabolomics, 11, pp.1095~1105 (2015)
- Sato H, "Stromata production for Cordyceps militaris(Clavicipitales : Clavicipitaceae)by injection of hyphal bodies to alternative host insects", Appl. Entomol. Zool, 37, pp.85~92 (2002)
- Talukder MR, "Light-emitting diodes and exogenous amino acids application improve growth and yield of strawberry plants cultivated in recycled hydroponics", Sci. Hortic, 239, pp.93~103 (2018)
- Viršilė A, "The distinct impact of multi-color LED light on nitrate, amino acid, soluble sugar and organic acid contents in red and green leaf lettuce cultivated in controlled environment", Food Chem, 310, pp.125799~ (2020)
- Wang JF, "Study on monosaccharide composition of intracellular polysacchride and contents of cordycepin and cordyceps polysacchride produced by Cordyceps militaris induced by blue light", Zhong Yao Cai, 37, pp.1395~1399 (2014)
- Wilawan N, "Changes in enzyme activities and amino acids and their relations with phenolic compounds contents in okra treated by LED lights of different colors", Food Bioproc. Tech, 12, pp.1945~1954 (2019)
- Willemse J, "SParticle, an algorithm for the analysis of filamentous microorganisms in submerged cultures", Antonie Van Leeuwenhoek, 111, pp.171~182 (2018)
- Wu G., "Amino acids : Metabolism, functions, and nutrition", Amino Acids, 37, pp.1~17 (2009)
- Yang T, "The blue-light receptor CmWC-1 mediates fruit body development and secondary metabolism in Cordyceps militaris", Appl. Microbiol. Biotechnol, 100, pp.743~755 (2016)
- Zhang T, "Effects of different LED sources on the growth and nitrogen metabolism of lettuce", Plant Cell Tissue Organ Cult, 134, pp.231~240 (2018)
