Biological Activities and Metabolite Analysis of Various Extracts and Fractions from Red Ginseng Marc
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초록
Red ginseng marc (RGM) has been used on primary industries using fertilizer or forage, and it mostly has beendumped. To improve utilization of RGM, the biological activities of RGM were examined. RGM was extracted andfractionated using various solvents and their biological activities were compared. The hexane fraction from the methanolextract of RGM (RGMMH) showed strong anti-cancer activity (58.56 ± 6.04% at 100 ㎍/mL) and anti-inflammatory effect(65.72 ± 1.33% at 100 ㎍/mL). But, oil extract of RGM extracted with hexane (RGMH) showed low activities (anti-cancer:16.42 ± 3.33%, at 100 ㎍/mL, anti-inflammatory activity: 29.46 ± 2.10%, at 100 ㎍/mL). Their metabolites were analyzedusing HPLC. Panaxydol known as anti-cancer compound of RGM was one of major compounds in RGMMH. Meanwhile,panaxydol was detected in trace amount in red ginseng marc oil (RGMH). In addition, RGMMH and RGMH showed bigdifferences in HPLC profiling. This research suggests optimal extraction method of RGM oil.
Abstract
Red ginseng marc (RGM) has been used on primary industries using fertilizer or forage, and it mostly has beendumped. To improve utilization of RGM, the biological activities of RGM were examined. RGM was extracted andfractionated using various solvents and their biological activities were compared. The hexane fraction from the methanolextract of RGM (RGMMH) showed strong anti-cancer activity (58.56 ± 6.04% at 100 ㎍/mL) and anti-inflammatory effect(65.72 ± 1.33% at 100 ㎍/mL). But, oil extract of RGM extracted with hexane (RGMH) showed low activities (anti-cancer:16.42 ± 3.33%, at 100 ㎍/mL, anti-inflammatory activity: 29.46 ± 2.10%, at 100 ㎍/mL). Their metabolites were analyzedusing HPLC. Panaxydol known as anti-cancer compound of RGM was one of major compounds in RGMMH. Meanwhile,panaxydol was detected in trace amount in red ginseng marc oil (RGMH). In addition, RGMMH and RGMH showed bigdifferences in HPLC profiling. This research suggests optimal extraction method of RGM oil.
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