BALB/c 마우스에서 발효 홍삼 Ginsenoside의 생체이용율과 항염효과
Bioavailability and Anti-inflammatory Effect of Fermented Red Ginseng in BALB/c Mouse
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본 연구에서는 발효홍삼과 발효하지 않은 홍삼을 BALB/c mouse에 경구투여한 후 혈청을 채취하여 주요 진세노사이드 생체이용율을 LC-MS/MS이용하여 분석하였다. 또한 이들의 항염효과를 IL-1β, TNF 정량분석을 통해 비교하였다. 홍삼을 발효하였을 때, Rd를 포함한 전체적인 총 진세노사이드 함량이 증가하는 것을 확인하였으며, 마우스에게 투여하였을 총 진세노사이드 TG 의 혈액 검출량 AUC 또한 발효홍삼을섭취하였을 때 높은 것을 확인하였다. Cmax값 또한 동일하게 발효홍삼을 섭취하였을 때 증가하는 것을 확인하였다. 염증지표를 확인 하였을때 유도군과 비교하여 IL-1β, TNF의 감소효과는 확인하였으나 그룹간의 유의적 차이는 발생하지 않았다. 이상의 연구결과로 probiotics가 발효홍삼의 ginsenoside 생체이용율을 향상시키는 중요한 요인임을 확인하였고, 이는probiotics를 이용한 천연물 발효제제의 적용 확장과 그 산업적활용성 증대에 기여할 수 있을 것으로 사료된다.
Abstract
The fermented red ginseng by microorganism is known to increase pharmacological activity in vivo. To evaluate the bioavailablity of red ginseng fermented by probiotics, we conducted the pharmacokinetic study of ginsenoside Rb1, Rd and total ginsenosides (TG, ginsenosides Rb1 + Rd + Rg1 + F2 + Rg3 + compound K) in BALB/C mice. The AUC value of ginsenoside Rb1 in mice serum administered with 600㎎/㎏ drugs showed 21.93 ± 14.68 ng·h/mL (RGw, water extract), 275.211 ± 110.04 ng·h/mL (RGe, 50% ethanol extract) and 404.91 ± 162.57 ng·h/mL (fRGe, fermented red ginseng extract). Analysis of ginsenoside Rd also showed a higher ACU value in fRGe than in RGw or RGe. And the AUC value of total ginsenosides in mice serum treated with 600 ㎎/㎏ were observed 42.12 ± 23.44 ng·h/mL (RGw), 321.44 ± 133.5 ng·h/mL (RGe) and 537.33 ± 229.01 ng·h/mL (fRGe), respectively. Cmax value of ginsenoside Rb1 in mice administered with 600㎎/ ㎏ were observed 3.67 ± 3.34 ng/mL (RGw), 23.27 ± 8.81 ng/mL (RGe) and 25.52 ± 7.29 ng/mL (fRGe). These results can be considered that the fermented red ginseng has more bioavailability than that of unfermented red ginseng. In quantitative analysis of the inflammation-related cytokines IL-1β and TNF, no significant difference was found between the fermented red ginseng (fRGe) and the red ginseng (RGe).
참고문헌 (26)
- Ahn, N.G., "Changes in chemical composition during red ginseng manufacturing process", MS, Department of Food Engineering, pp.2015
- Akao, T., "Intestinal bacterial hydrolysis is required for the appearance of compound K in rat plasma after oral administration of ginsenoside Rb1 from Panax ginseng", J. Pharm. Pharmacol, 50, pp.1155~1160 (1998)
- Cho, J. Y, "Immuno-regulatory efficacy or red ginseng derived ingredients", Food Preservation and Processing Industry, pp.6~12 (2009)
- Choi, J. H., "Variation of ginseng extract by heat treatment", J. Korean Agricultural Chemical Society, 24(1), pp.50~58 (1981)
- 최지원, "기호성 개선 인삼농축액 제조를 위한 추출 조건 설정", 한국식품저장유통학회지, 23(3), pp.319~325 (2016), 한국식품저장유통학회
- Choi, S. Y., "Fermented Korean red ginseng extract enriched in Rd and Rg3 protects against non-alcoholic fatty liver disease through regulation of mTORC1", Nutrients, 11, pp.2963~ (2019)
- Hasegawa, H., "Pharmacokinetics of ginsenoside deglycosylated by intestinal bacteria and its transformation to biologically active fatty acid esters", Biol. Pharm. Bull, 23(3), pp.298~304 (2000)
- Youl Her, "An Application of β-glycosidase to Transformation of Ginsenosides for the Effective Production of Specific Ginsenosides with Biological Efficacy", Biotechnology and Bioprocess Engineering, 17(3), pp.538~546 (2012), 한국생물공학회[DOI]
- Hye In Kim, "Fermented red ginseng and ginsenoside Rd alleviate ovalbumininduced allergic rhinitis in mice by suppressing IgE, interleukin-4, and interleukin-5 expression", Journal of Ginseng Research, 43(4), pp.635~644 (2019), 고려인삼학회[DOI]
- 김지혜, "Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responses and diseases", Journal of Ginseng Research, 41(4), pp.435~443 (2017), 고려인삼학회[DOI]
- Kim, J. K., "Fermented red ginseng alleviates cyclophosphamide-induced immunosuppression and 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice by regulation macrophage activation and T cell differentiation", Am. J. Chin. Med, 48(8), pp.1879~1897 (2018)
- Jeon-Kyung Kim, "Effects of gut microbiota on the pharmacokinetics of protopanaxadiol ginsenosides Rd, Rg3, F2, and compound K in healthy volunteers treated orally with red ginseng", Journal of Ginseng Research, 44(4), pp.611~618 (2020), 고려인삼학회[DOI]
- 김경아, "A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats", Journal of Ginseng Research, 39(2), pp.183~187 (2015), 고려인삼학회
- 김수철, "추출용매에 따른 산양삼의 진세노사이드(Rg1, Rb1) 함량과 radical-scavenging 활성 비교", 한국식품저장유통학회지, 28(2), pp.261~269 (2021), 한국식품저장유통학회
- 고성권, "백삼 및 홍삼 농축액의 사포닌 분석", 한국식품과학회지, 35(3), pp.536~539 (2003), 한국식품과학회
- 이강선, "추출방법에 따른 홍삼추출액의 사포닌 조성과 품질특성", 한국약용작물학회지, 21(4), pp.276~281 (2013), 한국약용작물학회[DOI]
- So Jin Lee, "Changes in the ginsenoside content during the fermentation process using microbial strains", Journal of Ginseng Research, 39(4), pp.392~397 (2015), 고려인삼학회
- 李翔国, "홍삼 물 추출액의 사포닌 함량 및 맛의 증진을 위한 추출 조건", 한국작물학회지, 54(3), pp.287~293 (2009), 한국작물학회
- Nam, K.Y, "The main ingreadients and efficacy of Koryeo red ginseng", Korean J. Plant Resources, 16(1), pp.33~39 (2009)
- Park, H., "The role of gut microbiota in ginsenoside metabolism and biotransformatino of ginsenoside by lactic acid bacteria", Curr. Top. Lact. Acid Bact. Probiotics, 5(1), pp.1~12 (2019)
- Saba, E., "Anti-inflammatory activity of Rg3-enriched Korean red ginseng extract in murine model of sepsis", Evid. Based Complement. Alternat. Med, 2018, pp.6874692~ (2018)
- 심건섭, "β-Glucosidase 생산 유산균주의 분리와 이를 이용한 팽화홍삼 추출액의 생물전환", 산업식품공학, 18(4), pp.332~340 (2014), 한국산업식품공학회[DOI]
- Trinh, H.T., "Intestinal microflora enhance the anti-pruritic activities of several glycosides from herbal medicines", Ph.D., Department of Pharmacy, pp.2011
- U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research, "Guidance for industry estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers", 1~27 (2005)
- Wakabayashi, C., "In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration", Oncol. Res, 9, pp.411~417 (1997)
- Xiaoyan Zhang, "Prebiotics enhance the biotransformation and bioavailability of ginsenosides in rats by modulating gut microbiota", Journal of Ginseng Research, 45(2), pp.334~343 (2021), 고려인삼학회[DOI]