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발효인삼의 Ginsenoside 변화와 항산화 활성

Ginsenoside Change and Antioxidation Activity of Fermented Ginseng

이건희|장준복|성낙술|도은수
한국약용작물학회|2010.08|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제18권 제4호|pp. 255-265

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참고문헌

키워드

AntioxidantFermentationGinsengCompound KGinsenoside

초록

The extent of growth L. plantarum (LP), L. delbrueckii subsp. bulgaricus (LD), L. fermentum (LF), S. thermophilus (ST), B. longum (BI) and S. cerevisiae (SA) was generally good with the lower concentration of the ginseng extract. Total sapogenin content was slightly different with kinds of a fermentation microorganism and the time of fermentation process,and generally reduced compare to before fermentation. The content of ginsenoside Rb1, Rb2, Rb3, Re and Rf were decreased with the fermentation but ginsenoside Rd was increased by the E, LF and SA fermented extract. The content of compound K increased in the order of not-fermented extrac < enzyme fermented extract < enzyme and microorganism fermented extract, and as the fermented time get longer, the content of compound K was sightly increased. Especially, the content of compound K of the SA fermented extract was the most increased, also it of the BI, LD and LF fermented extract was increased, so these extract were considered a high valuable. Polyphenol content of the BI, LD, LP and ST fermented extract indicated 9.18 ± 0.39~15.68 ± 0.54 ㎎/10 g which was lower than it of a not-fermented extract (11.92 ± 0.26~28.41 ± 0.39㎎/10 g). Flavonoid content of a ginseng fermented extract indicated 26.93 ± 0.17~156.45 ± 1.29 ㎎/10 g, it was higher than a not-fermented extract (18.06 ± 0.90 ㎎/10 g). As the fermented time get longer, the flavonoid content tendency to increase. DPPH radical scavenging activity of a fermented ginseng extract was 24.11 ± 1.41~ 55.62 ± 0.33%, it was slightly lower compared to a natural antioxidant, vitamin C. But it of the LF and ST fermented extract was similar to a natural antioxidant,vitamin C. It has not a concerned in a fermentation. Nitrite scavenging ability of a 24 hr fermented extract was above 80%at pH 2.5 and 4.2, it was similar to an artificial antioxidant, BHT (84.76 ± 0.13%; pH2.5, 84.98 ± 0.11%; pH 4.2). It has not a concerned in a fermentation. SOD-like activity of a fermented extract was lower than that of a not-fermented extract (19.22 ± 0.51%), but it of the E and LP-fermented extract was a very highly notable value. As the fermented time get longer,the SOD-like activity tendency to increase.

Abstract

The extent of growth L. plantarum (LP), L. delbrueckii subsp. bulgaricus (LD), L. fermentum (LF), S. thermophilus (ST), B. longum (BI) and S. cerevisiae (SA) was generally good with the lower concentration of the ginseng extract. Total sapogenin content was slightly different with kinds of a fermentation microorganism and the time of fermentation process,and generally reduced compare to before fermentation. The content of ginsenoside Rb1, Rb2, Rb3, Re and Rf were decreased with the fermentation but ginsenoside Rd was increased by the E, LF and SA fermented extract. The content of compound K increased in the order of not-fermented extrac < enzyme fermented extract < enzyme and microorganism fermented extract, and as the fermented time get longer, the content of compound K was sightly increased. Especially, the content of compound K of the SA fermented extract was the most increased, also it of the BI, LD and LF fermented extract was increased, so these extract were considered a high valuable. Polyphenol content of the BI, LD, LP and ST fermented extract indicated 9.18 ± 0.39~15.68 ± 0.54 ㎎/10 g which was lower than it of a not-fermented extract (11.92 ± 0.26~28.41 ± 0.39㎎/10 g). Flavonoid content of a ginseng fermented extract indicated 26.93 ± 0.17~156.45 ± 1.29 ㎎/10 g, it was higher than a not-fermented extract (18.06 ± 0.90 ㎎/10 g). As the fermented time get longer, the flavonoid content tendency to increase. DPPH radical scavenging activity of a fermented ginseng extract was 24.11 ± 1.41~ 55.62 ± 0.33%, it was slightly lower compared to a natural antioxidant, vitamin C. But it of the LF and ST fermented extract was similar to a natural antioxidant,vitamin C. It has not a concerned in a fermentation. Nitrite scavenging ability of a 24 hr fermented extract was above 80%at pH 2.5 and 4.2, it was similar to an artificial antioxidant, BHT (84.76 ± 0.13%; pH2.5, 84.98 ± 0.11%; pH 4.2). It has not a concerned in a fermentation. SOD-like activity of a fermented extract was lower than that of a not-fermented extract (19.22 ± 0.51%), but it of the E and LP-fermented extract was a very highly notable value. As the fermented time get longer,the SOD-like activity tendency to increase.

참고문헌 (56)

  1. Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 26, pp.1199~1200 (1958)
  2. 부희옥, "여주 품종별 폴리페놀, 플라보노이드 함량과 라디칼 소거활성 및 아질산염 소거능", 한국약용작물학회지, 17(1), pp.15~20 (2009), 한국약용작물학회
  3. Cha JY, "Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata", Journal of the Korean Society of Food Science and Nutrition, 28, pp.1310~1315 (1999)
  4. Cho HO, "Approach to the extraction method on minerals of ginseng extract", Korean Journal of Food Science and Technology, 8, pp.95~106 (1976)
  5. Choi HJ, "Changes in chemical compositions of green tea(Camellia sinensis L.)under the different extraction conditions", Journal of Life Science, 10, pp.202~209 (2000)
  6. Choi JS, "A naphthalene glycoside from Cassia tora", Phytochemistry, 40, pp.997~999 (1995)
  7. 최소라, "사철쑥의 항산화성과 항균성", 한국약용작물학회지, 16(2), pp.112~117 (2008), 한국약용작물학회
  8. Folin O, "On phosphotungastic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, 12, pp.239~249 (1912)
  9. Goldin BR, "Health benefits of probiotics", British Journal of Nutrition, 80, pp.203~207 (1998)
  10. Greenblatt M, "Nitrosamine studies:induction of lung adenomas by concurrent administration of sodium nitrite and secondary amines in Swiss mice", Journal of National Cancer Institute, 46, pp.1029~1034 (1971)
  11. 한승호, "국내 자생 식물 추출물의 항산화 활성 및 항균효과", 한국약용작물학회지, 14(1), pp.49~55 (2006), 한국약용작물학회
  12. 허성일, "민들레 추출물의 항산화 활성 및 세포독성 효과", 생약학회지, 39(3), pp.255~259 (2008), 한국생약학회
  13. Hong HD, "Superoxide dismutaselike activity of apple juice mixed with some fruits and vegetables", Journal of Food Science Technology, 30, pp.1484~1487 (1998)
  14. Hotchkiss JH, "A review of current literature on N-nitroso compounds in foods", Advanced of Food Research, 31, pp.54~115 (1998)
  15. 현재석, "진귤 및 온주밀감 진피의 항산화 활성", 한국식품영양과학회지, 39(1), pp.1~7 (2010), 한국식품영양과학회
  16. 신정혜, "한약재 열수추출물의 항산화 활성", 한국식품영양과학회지, 35(1), pp.7~14 (2006), 한국식품영양과학회
  17. 정미정, "인진쑥 추출물의 항산화 및 항암 활성", 생약학회지, 39(3), pp.194~198 (2008), 한국생약학회
  18. 정성제, "약용 식물 추출물의 항산화 활성 검색", Journal of the Korean Society for Applied Biological Chemistry, 47(1), pp.135~140 (2004), 한국응용생명화학회
  19. Kato H, "Inhibition of nitrosamine formation by nondialyzable melanoidines", Agricultural and Biological Chemistry, 51, pp.1333~1338 (1987)
  20. Kawaguchi, K., "Hesperidin as an inhibitor of lipases from porcine pancreas and pseudomonas", Bioscience,Biotechnology,and Biochemistry, 61, pp.102~104 (1997)
  21. Kim DY, "Studies on the browning of red ginseng", Journal of the Korean Society of Agricultural Chemistry and Biotechnology, 16, pp.60~77 (1973)
  22. 김은영, "항산화활성을 나타내는 약용식물 소재 탐색", 한국식품과학회지, 36(2), pp.333~338 (2004), 한국식품과학회
  23. 김희규, "Ginsenoside 전환이 가능한 인삼 발효 미생물의 선별", 미생물학회지, 43(2), pp.142~146 (2007), 한국미생물학회
  24. 한명주, "Bifidobacterium을 이용한 인삼 요구르트의 개발", 한국식품조리과학회지, 21(5), pp.575~584 (2005), 한국식품조리과학회
  25. Kim JY, "Antioxidative effects of soybean extracts by using various solvents", Korean Journal of Food Science and Technology, 27, pp.635~637 (1995)
  26. 김재영, "수종의 한약재 추출물의 항산화 활성 및 항진균 활성", 한국미생물·생명공학회지, 37(1), pp.42~48 (2009), 한국미생물·생명공학회
  27. 노정해, "효소처리와 열처리에 의한 인삼 추출물의 항산화 활성", 한국식품영양과학회지, 36(11) (2007), 한국식품영양과학회
  28. 공병만, "백삼, 발효인삼, 홍삼 농축액의 이화학적 특성", Journal of Ginseng Research, 32(3), pp.238~243 (2008), 고려인삼학회
  29. 이명렬, "연근 추출물의 항산화 효과", 생명과학회지, 17(9), pp.1237~1243 (2007), 한국생명과학회
  30. 이승은, "인삼의 부위별 항산화 활성", 한국약용작물학회지, 12(3), pp.237~242 (2004), 한국약용작물학회
  31. 이승은, "식물자원의 항산화활성 탐색", 한국약용작물학회지, 16(5), pp.356~370 (2008), 한국약용작물학회
  32. 이승호, "추출조건에 따른 홍삼추출액의 사포닌 조성 및 이화학적 특성", 한국식품영양과학회지, 37(2), pp.256~260 (2008), 한국식품영양과학회
  33. 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
  34. 차재영, "함초(Salicornia herbacea L.)의 채취 시기별 이화학적 특성 탐색", 생명과학회지, 16(4), pp.683~690 (2006), 한국생명과학회
  35. 이양숙, "만형자(Vitex rotundifolia) 추출물의 항산화 활성과 Tyrosinase 저해 활성", 한국식품저장유통학회지, 16(1), pp.101~108 (2009), 한국식품저장유통학회
  36. 김남우, "백지의 항산화성 및 생리기능", 한국식품영양과학회지, 36(1), pp.20~26 (2007), 한국식품영양과학회
  37. Lee JH, "Analysis of phenolic substances content on Korea plant foods", Korean Journal of Food Science and Technology, 26, pp.310~316 (1994)
  38. 임진아, "배암차즈기 메탄올 추출물의 항산화 활성 및 아질산염 소거능", 한국약용작물학회지, 15(3), pp.183~188 (2007), 한국약용작물학회
  39. 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교", 한국약용작물학회지, 12(3), pp.191~202 (2004), 한국약용작물학회
  40. 김은영, "항산화활성을 나타내는 약용식물 소재 탐색", 한국식품과학회지, 36(2), pp.333~338 (2004), 한국식품과학회
  41. Kim SY, "Antioxidant activities of selected oriental herb extracts", Journal of the American Oil Chemists' Society, 71, pp.633~640 (1994)
  42. Marklund S, "Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, 47, pp.469~474 (1974)
  43. Middleton E, "Potential healthpromoting properties of citrus flavonoids", Food Technology, 48, pp.115~119 (1994)
  44. 문지숙, "약용식품 추출에 대한 항산화성과 알코올 탈수소효소 저해성 연구", 한국식품저장유통학회지, 11(2), pp.201~206 (2004), 한국식품저장유통학회
  45. 김선재, "약용식품 추출에 대한 항미생물 활성 검색과 폴리페놀 함량", 한국식품저장유통학회지, 11(2), pp.207~213 (2004), 한국식품저장유통학회
  46. Nakagawa M, "Evaluation method of green tea grade by nitrogen analysis", Journal of Japanese Food Science and Technology, 21, pp.57~63 (1974)
  47. 박찬성, "한약재 추출물의 항산화작용 및 아질산염 소거작용", 한국식품저장유통학회지, 12(6), pp.631~636 (2005), 한국식품저장유통학회
  48. 박진철, "홍국발효홍삼의 이화학적 특성 및 생리활성", Journal of Life Science, 19(11), pp.1553~1561 (2009)
  49. 박수진, "유산균을 이용한 발효인삼 제조 및 품질 특성", Journal of Ginseng Research, 30(2), pp.88~94 (2006), 고려인삼학회
  50. Salim AS, "Oxygen-derived free radicals and the prevention of duodenal ulcer relapse", American Journal of the Medical Sciences, 300, pp.1~6 (1990)
  51. 서훈석, "짚신나물, 삼백초의 항산화와 항암활성 효과", 한국약용작물학회지, 16(3), pp.139~143 (2008), 한국약용작물학회
  52. 서훈석, "머위(Petasites japonicus) 추출물의 항산화와 항암활성 효과", 한국자원식물학회지, 21(4), pp.265~269 (2008), 한국자원식물학회
  53. 송재환, "찔레 영지버섯(Phellinus ribis) 추출물의 생리활성", 한국식품과학회지, 35(4), pp.690~695 (2003), 한국식품과학회
  54. 우관식, "반응표면분석에 의한 유자씨 에탄올 추출물의 항산화활성", 한국식품영양과학회지, 38(3), pp.384~390 (2009), 한국식품영양과학회
  55. 유진균, "목단피 물 추출물의 항산화 및 Tyrosinase 억제효과", 한국식품영양과학회지, 38(3), pp.292~296 (2009), 한국식품영양과학회
  56. Zhishen J, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chemistry, 64, pp.555~559 (1999)

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