금화규(Abelmoschus manihot) 뿌리 추출물의 면역증진 및 항비만효과
Immuno-enhancing and Anti-obesity Effect of Abelmoschus manihot Root Extracts
0
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
본 연구에서 금화규 뿌리 추출물(AMR)이 마우스 대식세포인RAW264.7 세포의 활성화 유도를 통한 면역증진 활성과 마우스지방전구세포인 3T3-L1 세포의 지질축적억제를 통한 항비만활성을 평가하였다. 금화규 뿌리 추출물(AMR)은 전반적으로RAW264.7 세포에서 TLR2/TLR4의 자극을 통해 p38과 JNK를활성화시켜 NO, iNOS, IL-1β, IL-6, TNF-α와 같은 면역증진인자의 발현을 증가시키는 것으로 판단된다. 그러나 IL-6의 경우, p38과 JNK 활성화에 의존하지 않는 것으로 확인되어TLR2/4에 의한 다른 신호전달이 관여하는 것으로 사료되어 추가적인 연구가 필요하다. 또한, 금화규 뿌리 추출물(AMR)은PPARγ의 과대발현을 억제하여 지방전구세포의 성숙한 지방세포로의 분화를 억제하고, 성숙한 지방세포에서 CEBPα, PPAR γ, perilipin-1, FABP4, adiponectin의 발현을 억제하여 지방세포 내 지질 형성 및 축적을 억제하는 것으로 판단된다. 본 연구를 통해 구명된 결과들은 금화규 뿌리 추출물(AMR)이 향후면역증진 및 항비만을 위한 보조제 또는 건강 기능성 식품과 의약품으로의 개발 및 활용이 가능할 것으로 생각된다.
Abstract
In this study, we investigated in vitro immune-enhancing and anti-obesity activity of Abelmoschus manihot roots (AMR) in mouse macrophage RAW264.7 cells and mouse adipocytes 3T3-L1 cells. AMR increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in RAW264.7 cells. The inhibition of toll like receptor (TLR) 2 and 4 blocked AMR-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of mitogen-activated protein kinases (MAPKs) signaling pathway reduced AMR-mediated production of immunostimulatory factors. From these results, AMR is considered to have immune-enhancing activity through TLR2/4-mediated activation of MAPKs signaling pathway. In addition, AMR inhibited lipid accumulation and reduced the protein level such as CCAAT enhancer-binding protein alpha (CEBPα), peroxisome proliferator-activated receptor gamma (PPARγ), perilipin-1, adiponectin and fatty acid binding protein 4 (FABP4) associated with lipid accumulation in 3T3-L1 cells, indicating that AMR may have anti-obesity activity. Based on these results, AMR is expected to be used as a potential functional agent for immune enhancement and anti-obesity.
참고문헌 (24)
- Alagawany, M., "The strategy of boosting the immune system under the COVID-19 pandemic", Front. Vet. Sci, 7, pp.570748~ (2021)
- Bandaru, P., "The impact of obesity on immune response to infection and vaccine : an insight into plausible mechanisms", Endocrinol. Metab. Synd, 2, pp.2~ (2013)
- Bogdan, C., "Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity", Curr. Opin. Immunol, 12(1), pp.64~76 (2000)
- Cuadrado, A., "Mechanisms and functions of p38 MAPK signalling", Biochem. J, 429(3), pp.403~417 (2010)
- Cuthbertson, D. J., "COVID-19and obesity : an opportunity for change", Ther. Adv. Endocrinol. Metab, 11, pp.1~4 (2020)
- Duque, G.A., "Macrophage cytokines: involvement in immunity and infectious diseases", Front. Immunol, 5, pp.491~ (2014)
- Erridge, C., "Endogenous ligands of TLR2 and TLR4:agonists or assistants?", J. Leukoc. Biol, 87(6), pp.989~999 (2010)
- Furuhashi, M., "Fatty acid-binding protein 4(FABP4) : pathophysiological insights and potent clinical biomarker of metabolic and cardiovascular diseases", Clin. Med. Insights Cardiol, 8(3), pp.23~33 (2014)
- Hansen, J. S., "Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes", Sci. Rep, 7(1), pp.1~14 (2017)
- Hussain, A., "Obesity and mortality of COVID-19. Meta-analysis", Obes. Res. Clin. Pract, 14(4), pp.295~300 (2020)
- 전연홍, "금화규(Abelmoschus manihot jinhuakui) 추출물의 화장품 소재로서의 응용", 융합정보논문지, 10(10), pp.290~297 (2020), 중소기업융합학회[DOI]
- Kadowaki, T., "Adiponectin and adiponectin receptors", Endocr. Rev, 26(3), pp.439~451 (2005)
- Kawai, T., "TLR signaling", Semin. Immunol, 19(1), pp.24~32 (2007)
- Kopelman, P. G, "Obesity as a medical problem", Nature, 404, pp.635~643 (2000)
- Kosaraju, R., "B cell activity is impaired in human and mouse obesity and is responsive to an essential fatty acid upon murine influenza infection", J. Immunol, 198(12), pp.4738~4752 (2017)
- Kyriakis, J. M., "Mammalian MAPK signal transduction pathways activated by stress and inflammation : a 10-year update", Physiol. Rev, 92(2), pp.689~737 (2012)
- Roskoski Jr, R, "ERK1", 2 MAP kinases: structure, function, and regulation, 2012, pp.6(2), 105~143
- Seki, E., "A liver full of JNK:signaling in regulation of cell function and disease pathogenesis, and clinical approaches", Gastroenterology, 143(2), pp.307~320 (2012)
- 서현주, "Immune-Enhancing Effects of Green Lettuce (Lactuca sativa L.) Extracts through the TLR4-MAPK", NF-κB Signaling Pathways in RAW264.7 Macrophage Cells, 2020, pp.33(3), 183~193[DOI]
- Simu, S. Y., "Ginsenoside Rg5: Rk1 exerts an anti-obesity effect on 3T3-L1cell line by the downregulation of PPARγ and CEBPα", Iran. J. Biotechnol, 15(4), pp.252~ (2017)
- Takeda, K., "TLR signaling pathways", Semin. Immunol, 16(1), pp.3~9 (2004)
- Wang, T., "TNF-α and IL-6 : The link between immune and bone system", Curr. Drug Targets, 21(3), pp.213~227 (2020)
- Weber, A., "Interleukin-1β(IL-1β)processing pathway", Sci. Signal, 3(105), pp.2~ (2010)
- Zhang, W., "MAPK signal pathways in the regulation of cell proliferation in mammalian cells", Cell Res, 12(1), pp.9~18 (2002)