추출용매에 따른 레몬밤 추출물의 중성지방 조절 효능 검증
Verification of the Effect of Lemon Balm Extract on Triglyceride Control According to the Extraction Solvent
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본 연구는 추출용매에 따른 레몬밤 추출물의 중성지방 조절에 대한 논문으로 3T3-L1 지방전구세포를 이용해 지방세포로분화유도한 뒤 lipid accumulation, triglyceride contents, PKA, HSL, perilipin, ATGL 및 CGI-58 단백질 발현을 확인하였다. 실험결과 네가지 조건의 레몬밤 추출물 중 물 추출 조건인MOW100 추출물만 lipid accumulation과 triglyceride contents 의유의적 억제 효능을 나타내었으며, MOE70, MOE50, MOE50 추출물은 lipid accumulation과 triglyceride contents의 감소효과가 없거나 유의적 차이를 나타내지 않는 것이 확인되었다. MOW100 추출물의 세포내 지방 축적 억제는 지방세포 내lipolysis를 조절하는 효소 조절을 통해 나타난 결과로 판단된다. 따라서 레몬밤 물 추출물은 cAMP-PKA를 활성화시키고, lipolysis를 조절하는 효소의 상승작용을 통해 세포내 중성지방을 조절함으로써 혈중 중성지방을 개선하는 후보소재로의 사용이 가능할 것으로 사료된다.
Abstract
This study investigated the effect of lemon balm (Melissa officinalis) extract on improving blood triglycerides according to the extraction solvent using 3T3-L1 cells. Lemon balm was extracted with water (MOW100), 70% ethanol (MOE70), 50% ethanol (MOE50), and 30% ethanol (MOE30). To verify its efficacy on improving blood triglycerides, cell viability, lipid accumulation, triglyceride (TG) content, and expressions of protein kinase A (PKA), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), perilipin, and CGI-58 proteins were evaluated. Cytotoxicity was not evident up to an extract concentration of 1 ㎎/mL. Lipid accumulation and TG content were decreased in a concentration-dependent manner compared to their levels in the control group. When the MOW100 extract was applied at a concentration of 0.2 ㎎/mL, an inhibitory effect was evident, with lipid accumulation inhibited by 21.3% and TG content reduced by 32.7%. PKA phosphorylation and ATGL HSL and CGI-58 levels were increased. The data indicate that lemon balm extract obtained using water is more efficacious than extracted with ethanol. The aqueous extract shows potential in triglyceride control through lipolysis and lowering triglyceride levels.
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