자귀나무 잎 에탄올 추출물의 3T3-L1 지방전구세포 분화 억제
A study on the inhibitory effects of 3T3-L1 preadipocytes differentiation process with ethanol extracts of Albizia julibrissin leaves
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The present study aimed to investigate the effects of ethanol extracts of Albizia julibrissin leaves on the differentiation process of 3T3-L1 preadipocytes. For this study, two different ethanol concentration extracts (30, 95%) were prepared. Lipid accumulation tests using Oil Red O staining showed a decrease in a certain concentration range for each 30% and 95% ethanol extract. The 30% and 95% extracts suppressed triglyceride content up to 60% in a concentration dependent manner. Polyphenolic compounds were investigated in 30% and 95% extracts using LC/MS/MS. Hyperoside showed the highest content in both 30% and 95% extracts and Naringin showed a relatively higher content in the 95% extract than in the 30% extract. The effect of 95% extract on the expression of transcription factors PPARγ, C/EBPα and SREBP involved in the adipocyte differentiation stage was investigated. In the case of 3T3-L1 cells treated with 95% ethanol extract, the expression of PPARγ, C/EBPα and SREBP was significantly reduced compared to cells treated with differentiation medium alone in a concentration-dependent manner. Based on these results, the ethanol extracts of Albizia julibrissin leaves can be thought to have high potential for obesity.
Abstract
The present study aimed to investigate the effects of ethanol extracts of Albizia julibrissin leaves on the differentiation process of 3T3-L1 preadipocytes. For this study, two different ethanol concentration extracts (30, 95%) were prepared. Lipid accumulation tests using Oil Red O staining showed a decrease in a certain concentration range for each 30% and 95% ethanol extract. The 30% and 95% extracts suppressed triglyceride content up to 60% in a concentration dependent manner. Polyphenolic compounds were investigated in 30% and 95% extracts using LC/MS/MS. Hyperoside showed the highest content in both 30% and 95% extracts and Naringin showed a relatively higher content in the 95% extract than in the 30% extract. The effect of 95% extract on the expression of transcription factors PPARγ, C/EBPα and SREBP involved in the adipocyte differentiation stage was investigated. In the case of 3T3-L1 cells treated with 95% ethanol extract, the expression of PPARγ, C/EBPα and SREBP was significantly reduced compared to cells treated with differentiation medium alone in a concentration-dependent manner. Based on these results, the ethanol extracts of Albizia julibrissin leaves can be thought to have high potential for obesity.