아사이베리 에탄올 추출물과 열수 추출물의 생리활성 검증
A Verification of Physiological Activities between Acai Berry (Euterpe oleracea Mart.) Ethanol and Hot-water Extracts
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To determine the characteristics of acai berry extract as a natural functional ingredient, this study measured the following: extraction yield, total polyphenols, flavonoid contents, DPPH radical scavenging activity, nitric oxide scavenging activity and antimicrobial activity. According to the measurement, in terms of the extraction yield of acai berry, the hot-water extract was 28.52% while the ethanol extract was 19.47%. In terms of polyphenol content, the hot-water extract was 77.49±0.93 mg/g, and ethanol extract was 128.42±5.68 mg/g. In terms of flavonoid content, the hot-water and ethanol extracts were 2.49±1.02 mg/g and 35.33±1.02 mg/g, respectively. Overall, polyphenol content was greater than flavonoid content. In terms of antioxidant content, the ethanol extract was higher than the hot-water extract. In terms of DPPH radical scavenging activity at 5.0 mg/ml, the hot-water extract (73.02±0.56%) was lower than the ethanol extract (82.49±0.28%). In nitric oxide scavenging activity at 5.0 mg/ml as well, the hot-water extract (74.25±0.25%) was lower than the ethanol extract (85.22±1.75%). According to the measurement of antimicrobial activity on Staphylococcus aureus and Staphylococcus epidermidis at 5mg/disc using the paper-disc method, high antimicrobial effects were observed in both S. aureus and S. epidermidis. In addition, antimicrobial activity was greater for the ethanol extract than for the hot-water extract.
Abstract
To determine the characteristics of acai berry extract as a natural functional ingredient, this study measured the following: extraction yield, total polyphenols, flavonoid contents, DPPH radical scavenging activity, nitric oxide scavenging activity and antimicrobial activity. According to the measurement, in terms of the extraction yield of acai berry, the hot-water extract was 28.52% while the ethanol extract was 19.47%. In terms of polyphenol content, the hot-water extract was 77.49±0.93 mg/g, and ethanol extract was 128.42±5.68 mg/g. In terms of flavonoid content, the hot-water and ethanol extracts were 2.49±1.02 mg/g and 35.33±1.02 mg/g, respectively. Overall, polyphenol content was greater than flavonoid content. In terms of antioxidant content, the ethanol extract was higher than the hot-water extract. In terms of DPPH radical scavenging activity at 5.0 mg/ml, the hot-water extract (73.02±0.56%) was lower than the ethanol extract (82.49±0.28%). In nitric oxide scavenging activity at 5.0 mg/ml as well, the hot-water extract (74.25±0.25%) was lower than the ethanol extract (85.22±1.75%). According to the measurement of antimicrobial activity on Staphylococcus aureus and Staphylococcus epidermidis at 5mg/disc using the paper-disc method, high antimicrobial effects were observed in both S. aureus and S. epidermidis. In addition, antimicrobial activity was greater for the ethanol extract than for the hot-water extract.
참고문헌 (39)
- 김단비, "음료 농축액 및 퓨레의 항산화 활성", 한국식품영양과학회지, 42(7), pp.997~1002 (2013), 한국식품영양과학회
- 문기원, "노인의 보완대체요법 이용행태 및 알로에(Aloe)와 천연물 복합추출물의 항산화 효과", 박사, 대체의학과, pp.2013
- AMes B. N., "Oxidative DNA danage, cancer and aging. Oxygen and human disease", Ann. Inter. Med, 107(1), pp.536~539 (1987)
- 손장락, "활성산소와 항산화제", pp.19~20 (2008), 바이오메디컬
- 한지연, "아사이베리 과편의 제조조건 최적화 및 품질특성", 석사, 식품영양학과 식품영양학전공, pp.2013
- 서수정, "미용소재 개발을 위한 남천(Nandina domestica) 열매 추출물의 항산화 효과", 대한미용학회지, 9(1), pp.7~14 (2013), 대한미용학회
- 황수영, "아사이베리(Euterpe oleracea Mart.)를 첨가한 전통주악의 이화학적·물리적 품질특성", 석사, 식품가공학과 외식산업학전공, pp.2013
- Patricia D.S. Spada, "Frozen Fruit Pulp of Euterpe oleraceae Mart. (Acai) Prevents Hydrogen Peroxide-Induced Damage in the Cerebral Cortex, Cerebellum, and Hippocampus of Rats", Journal of Medicinal Food, 12(5), pp.1084~1088 (2009), 한국식품영양과학회
- Jie Kang, "Anti-oxidant capacities of flavonoids compounds isolated from acai pulp", Food Chemistry, 122(3), pp.610~661 (2010)
- Lichtenthaler R, "Total oxidant scavengig capacities of Euterpe oleracea Mart(Acai)fruits Int", J. Food Sci. Nutr, 56(1), pp.53~64 (2005)
- 정해정, "추출용매에 따른 아사이 베리의 생리활성", 한국식생활문화학회지, 27(1), pp.75~81 (2012), 한국식생활문화학회
- Heinrich M, "Acai(Euterpe oleracea Mart.) a phytochemical and pharmacological assessment of the species' health claims", Phytochem Lett, 4(1), pp.10~21 (2011)
- 고경숙, "자화지정 추출물 적용에 따른 모발손상 및 형태학적 변화에 관한 연구", 한국미용학회지, 19(3), pp.411~419 (2013), 한국미용학회
- 김현희, "로즈마리 추출물의 생리활성 및 모발 염색성 연구", 박사, 뷰티디자인학과, pp.2014
- 김진란, "천마적용이 화학적 반복시술에 따른 모발 손상에 미치는 영향", 석사, 뷰티디자인학과, pp.2013
- 김미연, "화학적 시술시 로즈힙 추출물 첨가가 모발손상 및 아미노산 조성 변화에 미치는 영향", 석사, 뷰티디자인학과, pp.2012
- Swain T, "Phenolic constituents of Ptu-nus domestica I, quantitative analysis of phenolic cinstituents", J. Sci. Food Agri, 10, pp.83~88 (1959)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several region of Argentina", Journal of Ethnopharmacology, 71, pp.109~114 (2000)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181(1), pp.99~1200 (1958)
- Marcocci L, "The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761", Biochemical and Biophysical Research Communications, 201, pp.748~755 (1994)
- Dzink JL, "Comparative in vitro activity of sanguinarine against oral microbial isolates", Antimicrob Agents chemother, 27, pp.663~665 (1985)
- 이상일, "발효 청미래덩굴잎 용매 추출물 및 분획물의 xanthine 및 aldehyde oxidase 저해활성과 항고요산혈증 효과", Journal of Applied Biological Chemistry, 57(1), pp.53~59 (2014), 한국응용생명화학회
- 박성진, "씀바귀 에탄올 추출물의 항산화 및 지방세포 분화억제 효과", 한국조리학회지, 20(1), pp.133~142 (2014), (사)한국조리학회
- 박성진, "초고압 처리가 블루베리의 항산화 증진에 미치는 영향", 한국식품영양과학회지, 43(3), pp.471~476 (2014), 한국식품영양과학회
- 정민숙, "무 에탄올 추출물의 in vitro 생리활성 분석", Applied Biological Chemistry, 47(1), pp.67~71 (2004), 한국응용생명화학회
- 김현희, "로즈마리 추출물의 화장품 생리활성 및 세포 독성 연구", 한국인체미용예술학회지, 14(4), pp.235~248 (2013), 한국인체미용예술학회
- 모정희, "노근 에탄올과 열수 추출물의 항산화 활성비교", 한국미용학회지, 19(5), pp.809~814 (2013), 한국미용학회
- 김명곤, "참마(Discorea japonica) 추출물의 생리활성 비교", 한국미용학회지, 19(3), pp.509~515 (2013), 한국미용학회
- 박주아, "소리쟁이(Rumex crispus L.) 잎 추출물의 항산화 효과 및 각질세포 보호효과", 한국인체미용예술학회지, 12(2), pp.189~198 (2011), 한국인체미용예술학회
- 채인경, "가자, 라벤더, 강향의 항염증 및 항산화 활성 검색", 생명과학회지, 21(4), pp.561~567 (2011), 한국생명과학회
- 이인선, "약쑥(Artemisia princeps Pamp)의 항산화작용", 한국식품영양과학회지, 36(6), pp.657~662 (2007), 한국식품영양과학회
- 고경숙, "황금(黃芩) 추출물의 항산화 활성 및 세포독성 연구", 한국인체미용예술학회지, 14(2), pp.233~243 (2013), 한국인체미용예술학회
- 임도연, "서양민들레와 흰민들레 추출물의 Nitric Oxide 생성억제 및 소거 활성과 Tyrosinase 저해 활성", 한국약용작물학회지, 19(5), pp.362~367 (2011), 한국약용작물학회
- 최봉희, "미세분진이 흰쥐의 폐포대식세포에서 Nitric Oxide 생성 및 iNOS 발현과 Nitrotyrosilated-protein의 형성", Tuberculosis and Respiratory Diseases, 60(4), pp.426~436 (2006), 대한결핵및호흡기학회
- 지선옥, "상백피 추출물의 항산화 활성 및 미백효과", 한국자원식물학회지, 22(2), pp.145~151 (2009), 한국자원식물학회
- 최호형, "미생물학", pp.496~ (2004), 아카데미서적
- 김미연, "목단피 추출물의 화장품 약리활성 검증", 한국미용학회지, 19(6), pp.1119~1126 (2013), 한국미용학회
- 박춘근, "수종 한약재 추출물의 Staphylococcus aureus에 대한 항균활성", 한국약용작물학회지, 9(4), pp.251~258 (2001), 한국약용작물학회
- 김용숙, "천연화장품 소재 탐색을 위한 명감나무잎 추출물과 분획물의 피부 균주에 대한 항균활성 조사", 한국미용학회지, 19(3), pp.557~564 (2013), 한국미용학회