본문 바로가기
KERD
논문KCI 등재

十全大補湯과 加味十全大補湯이 뇌허혈 흰쥐의 뇌혈류역학에 미치는 실험적 영향

Experimental Effects of Sibjeondaebo-tang and Gamy-Sibjeondaebo-tang on Cerebral Hemodynamics in Cerebral Ischemia Rats

정현우|이상영
한의병리학회 (The Society of Pathology in Korean Medicine)|2013.04|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제27권 제2호|pp. 173-182

1

조회

0

다운로드

6

인용

0

참고문헌

키워드

BDNFCervi Pantotrichum CornuTTCregional cerebral blood flowcerebral ischemiaSibjeondaebo-tangmean arterial blood pressure
주제분류한의학

초록

This Study was designed to investigate the effects of Sibjeondaebo-tang (SDT) and Gamy-Sibjeondaebo-tang (GST, Sibjeondaebo-tang adding Cervi Pantotrichum Cornu) on the improvement in regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, and in the rats with cerebral ischemia induced by middle cerebral artery occlusion, and further to determine the mechanisms. And, It was to investigate the effects of the SDT and GST with the change of histologic examination through the BDNF in the hippocampus CA1. In changes of cerebral hemodynamics, SDT and GST significantly increased rCBF in a dose-dependent manner but decreased MABP in normal rats. In mechanism of cerebral hemodynamics, Increase of GST-induced rCBF was significantly inhibited by pretreatment with methylene blue (0.01 ㎎/㎏, i.p.), an inhibitor of guanylate cyclase, and Decrease of GST-induced MABP was significantly increased by pretreatment with methylene. These results suggested that the action of GST was mediated by guantlate cyclase pathway. In cerebral ischemics, the rCBF was stably improved by SDT (10 ㎎/㎏, i.p.) significantly and stably increased by GST (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrast with the findings of rapid and marked increase in Control group. These results suggested that GST had anti-ischemic action in cerebral ischemic state. In histological examination through TTC stain, Sample A group and Sample B group decreased discoloration in the cortical part at 28th day after MCAO induction. In immunohistochemistric response of BDNF, Sample A group and Sample B group increased respondent effect at 28th day after MCAO induction. These results suggest that GST can Increase rCBF in normal state, as well as improve the stability of rCBF in cerebral ischemic state. Furthermore, methylene blue inhibitor study suggested the mechanism of blood flow enhancement by GST may be mediated by guanylate cyclase pathway.

Abstract

This Study was designed to investigate the effects of Sibjeondaebo-tang (SDT) and Gamy-Sibjeondaebo-tang (GST, Sibjeondaebo-tang adding Cervi Pantotrichum Cornu) on the improvement in regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, and in the rats with cerebral ischemia induced by middle cerebral artery occlusion, and further to determine the mechanisms. And, It was to investigate the effects of the SDT and GST with the change of histologic examination through the BDNF in the hippocampus CA1. In changes of cerebral hemodynamics, SDT and GST significantly increased rCBF in a dose-dependent manner but decreased MABP in normal rats. In mechanism of cerebral hemodynamics, Increase of GST-induced rCBF was significantly inhibited by pretreatment with methylene blue (0.01 ㎎/㎏, i.p.), an inhibitor of guanylate cyclase, and Decrease of GST-induced MABP was significantly increased by pretreatment with methylene. These results suggested that the action of GST was mediated by guantlate cyclase pathway. In cerebral ischemics, the rCBF was stably improved by SDT (10 ㎎/㎏, i.p.) significantly and stably increased by GST (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrast with the findings of rapid and marked increase in Control group. These results suggested that GST had anti-ischemic action in cerebral ischemic state. In histological examination through TTC stain, Sample A group and Sample B group decreased discoloration in the cortical part at 28th day after MCAO induction. In immunohistochemistric response of BDNF, Sample A group and Sample B group increased respondent effect at 28th day after MCAO induction. These results suggest that GST can Increase rCBF in normal state, as well as improve the stability of rCBF in cerebral ischemic state. Furthermore, methylene blue inhibitor study suggested the mechanism of blood flow enhancement by GST may be mediated by guanylate cyclase pathway.

참고문헌 (46)

  1. 의과대학 신경해부학교수, "증례를 통한 임상신경해부학", pp.191~ (1995), 한우리
  2. Kety, S. S., "The nitrous oxide method for the man ; theory, procedure and normal values", J. Clin Invest, 27, pp.476~483 (1948)
  3. 이경은, "허혈, 재관류 손상에서 뇌조직 아민 변동과 Free Radical과의 관련성", 大韓神經科學會誌, 8(1), pp.2~8 (1990)
  4. 대한병리학회, "병리학", pp.87~99 (1994), 고문사
  5. 국무조정실에서 시행한 연구개발사업의 결과보고서 ; 뇌신경세포의 허혈성 및 산화적 손상에 대한 한방처방 치료 효능 연구 Ⅱ, "조동욱", 한국한의학연구원, ~ (2001)
  6. 김영석, "임상중풍학", pp.67~546 (1997), 서원당
  7. 方葯中, "實用中醫內科學", pp.420~ (1986), 上海科學技術出版社
  8. 洪性範, "高血壓 中風의 漢方療法", pp.305~306 (1983), 醫學社
  9. 柯雪帆, "上海中醫學院出版社", pp.176~179 (1987), 上海中醫學院出版社
  10. 朴英培, "傳統醫學硏究所", pp.248~253 (1995), 傳統醫學硏究所
  11. 許 浚, "東醫寶鑑", pp.365~447 (1983), 南山堂
  12. 정규만, "십전대보탕에 관한 문헌적 고찰", 동서의학, 5(2), pp.16~20 (1980)
  13. 全國韓醫科大學 本草學敎授, "본초학", pp.302~583 (1999), 永林社
  14. 이세나, "韓藥混合製劑와 鹿茸醱酵抽出物의 成長期 흰쥐 長骨 길이 成長에 대한 효과", 대한본초학회지, 24(1), pp.121~131 (2009), 대한본초학회
  15. 한상원, "녹용 및 녹용약침이 동물의 성장과 지능발달에 미치는 영향", 대한침구학회지, 18(5), pp.122~134 (2001)
  16. 이민형, "녹용의 조혈작용에 대한 실험적 연구", 대한본초학회지, 16(1), pp.91~109 (2001)
  17. 김성훈, "녹용의 품종에 따른 조혈작용 비교 연구", 생약학회지, 35(1), pp.6~15 (2004), 한국생약학회
  18. 이수영, "녹용대보탕의 노화 억제 효과에 대한 실험적 연구", 한의학논문집, 16(2), pp.327~348 (2007)
  19. Chen, S. T., "A model of focal ischemic stoke in the rat. reproducible extension cortical infarction", Stroke, 17, pp.738~743 (1986)
  20. Longa, E. Z., "Reversible middle cerebral artery occlusion without craniectomy in rats", Stroke, 20(1), pp.84~91 (1989)
  21. Bederson, J. B., "Rat middle cerebral artery occlusion ; evaluation of the model and development of a neurologic examination", Stroke, 17(3), pp.472~476 (1986)
  22. Gharbawie, O. A., "Skilled reaching impairments from the lateral frontal cortex component of middle cerebral artery stroke : a qualitative and quantitative comparison to focal motor cortex lesions in rats", Behav. Brain Res, 156(1), pp.125~137 (2005)
  23. Iwamoto, J., "Methylene blue inhibits hypoxic cerebral vasodilation in awake sheep", 2226-2232, 73(6), pp.2226~2232 (1992)
  24. Wang, Q., "Comparison of the effects of NG-nitro-L-arginine and indomethacin on the hypercapnic cerebral blood flow increase in rats", Brain Res, 641(2), pp.257~264 (1994)
  25. 통계청, "사망원인 통계 연보" (2008), 통계청
  26. 김세길, "風의 병리적 의미규명과 중풍의 원인 및 치료에 대한 동서의학적 비교", 대한한의학회지, 16(1), pp.96~117 (1995)
  27. Glanz, M., "Functional electrostimulation in poststroke rehabilitation ; a meta-analysis of the randomized controlled trials", Arch. Phys. Med. Rehabil, 77(6), pp.549~553 (1996)
  28. 황규동, "십전대보탕 와송 및 십전대보탕가와송의 항암효과와 면역반응에 관한 연구", 대한한방종양학회지, 2(1), pp.1~23 (1996)
  29. 고영권, "십전대보탕이 CD4+ 및 CD8+ T세포수에 미치는 영향", 대한한방종양학회지, 4(1), pp.111~129 (1998)
  30. 윤재호, "십전대보탕이 암전이 억제에 미치는 영향", 대한한방종양학회지, 4(1), pp.131~146 (1998)
  31. 임동주, "십전대보탕가미방의 면역조절효과에 관한 실험적 연구", 한의학논문집, 10(1), pp.55~65 (2001)
  32. 최승훈, "십전대보탕의 면역증강 및 항암 효과", 한의학논문집, 11(1), pp.257~283 (2002)
  33. 서울대학교 의과대학 내과학교실, "내과학", pp.146~158 (1996), 군자출판사
  34. 金祐謙, "인체의 생리", pp.30~118 (1985), 서울대학교 출판부
  35. 대한신경외과학회, "신경외과학", pp.150~299 (1997), 중앙문화사
  36. McCord, J.M., "Mechanisms of disease ; oxygen-derived free radicals in postischemic tissue injury", New Eng J. Med, 312, pp.159~163 (1985)
  37. Dirnagl, U., "Pathobiology of ischemic stroke ; An integrated view", Trends Neurosci, 22(9), pp.391~397 (1999)
  38. Traystman, R.J., "Animal models of focal and global cerebral ischemia", ILAR J, 44(2), pp.85~95 (2003)
  39. Lipton, P, "Ischemic cell death in brain neurons", Physiological Reviews, 79(4), pp.1431~1568 (1999)
  40. Aronowski, J., "Graded bioassay for demonstration of brain rescue from experimental acute ischemia in rats", Stroke, 25(11), pp.2235~2240 (1994)
  41. Goldlust, E. J., "Automated measurement of infarct size with scanned images of triphenyltetrazolium chloride-stained rat brains", Stroke, 27(9), pp.1657~1662 (1996)
  42. Palmer, P. M. J., "Nitric oxide release accounts for the biology activity of endothelium derived relaxing factor", Nature, 327, pp.524~526 (1990)
  43. Shibuki, K., "Endogenous nitric oxide release required for long term synaptic depression in the cerebellum", Nature, 349, pp.326~328 (1991)
  44. 김경환, "이우주의 약리학 강의", pp.82~442 (1998), 의학문화사
  45. 박철훈, "팔물탕이 뇌허혈 억제효과에 미치는 실험적 연구", 박사, 한의학, pp.2004
  46. 이석진, "녹용약침이 뇌허혈 유발 흰쥐의 뇌혈류역학적 변화에 미치는 영향", 박사, 한의학, pp.2009

관련 논문AI추천

가감총명탕이 흰쥐의 뇌혈류량 변화에 미치는 영향김경윤, 김계엽, 김행중, 김형우, 이상영, 정현우, 차대연한의병리학회 · 동의생리병리학회지2008 · 제22권 제2호愈風養營湯이 정상 흰쥐의 국소 뇌혈류량 및 평균혈압에 미치는 影響안가영, 전상윤, 조국령대한한방내과학회 · 대한한방내과학회지2009 · 제30권 제2호滋陰健脾湯加吳茱萸 추출물이 흰쥐의 뇌혈류역학에 미치는 영향공경희, 이기승, 이은경, 정명수, 정현우대한예방한의학회 · 대한예방한의학회지2015 · 제19권 제3호당귀와 백수오 추출물이 뇌혈류 변화에 미치는 영향송민선, 양동혁, 이상영, 정종길, 정현우, 최찬헌한의병리학회 · 동의생리병리학회지2013 · 제27권 제4호凉膈散火湯이 腦血流 및 腦虛血 損傷에 미치는 영향손상곤, 송일병, 신민규사상체질면역의학회 · 사상체질면역의학회지2001 · 제13권 제2호신유․지실 녹용약침이 뇌혈류역학에 미치는 영향이석진, 정현우한의병리학회 · 동의생리병리학회지2009 · 제23권 제1호

이 간행물의 다른 논문 (14건)

陽虛證의 임상적 질환 범위에 대한 고찰김영목, 박미선pp. 153-166腠理槪念에 근거한 피부과 질병의 진단지표 기초연구지규용pp. 167-172마 에탄올추출물의 피부 collagen 합성 촉진 및 MMPs 활성 억제효과임난영, 문연자, 전병국, 김대성, 이영은, 우원홍pp. 183-188桃枝 메탄올 추출물과 용매별 분획물이 활성산소종, 활성질소종 및 저밀도 지단백의 산화에 미치는 효과문진영, 박주연pp. 189-195고지방과 streptozotocin으로 유도한 제 2형 당뇨에서 표고버섯이 지질대사와 항당뇨 효능에 미치는 영향윤영제, 김은정, 김계엽pp. 196-201
원문 보기