檳榔子 추출물의 마우스 경구 단회 투여독성 평가
Single Oral Dose Toxicity Test of Areca catechu Aqueous Extracts in Mice
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This study was to evaluate the single dose toxicity of Arecae Semen (AS) in male and female ICR mice. Aqueous extracts of AS (Yield = 13.15%) were administered as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 12 principle organs were examined. We could not find any mortality, clinical signs, and changes in the body and organ weight except for diarrhea. Diarrhea were observed in all three different dosage groups of male mice, and in 2000 mg/kg groups of female mice within 48hrs after administration. In addition, no AS extract related abnormal gross findings and changes in histopathology of principle organs were detected except for some sporadic accidental findings. Although the 50% lethal dose and approximate lethal dose of AS aqueous extracts in female and male mice were detected as over 2,000 mg/kg – the limited highest dosage recommended by KFDA guidelines. It should be carefully used in clinics because AS may be induced severe digestive tract disorders.
Abstract
This study was to evaluate the single dose toxicity of Arecae Semen (AS) in male and female ICR mice. Aqueous extracts of AS (Yield = 13.15%) were administered as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 12 principle organs were examined. We could not find any mortality, clinical signs, and changes in the body and organ weight except for diarrhea. Diarrhea were observed in all three different dosage groups of male mice, and in 2000 mg/kg groups of female mice within 48hrs after administration. In addition, no AS extract related abnormal gross findings and changes in histopathology of principle organs were detected except for some sporadic accidental findings. Although the 50% lethal dose and approximate lethal dose of AS aqueous extracts in female and male mice were detected as over 2,000 mg/kg – the limited highest dosage recommended by KFDA guidelines. It should be carefully used in clinics because AS may be induced severe digestive tract disorders.
참고문헌 (24)
- 김동현, "한방약리학", pp.587~590 (2010), 신일상사
- 김인락, "본초학", pp.420~421 (2007), 영림사
- Ghayur, M. N., "Identification of antiplatelet and acetylcholinesterase inhibitory constituents in betel nut", Zhong Xi Yi Jie He Xue Bao, 9, pp.619~625 (2011)
- Bhandare, A., "Evaluation of anti-migraine potential of Areca catechu to prevent nitroglycerin-induced delayed inflammation in rat meninges: Possible involvement of NOS inhibition", J Ethnopharmacol, 136, pp.267~270 (2011)
- Khan, S., "Studies on anti-inflammatory and analgesic activities of betel nut in rodents", J Ethnopharmacol, 135, pp.654~661 (2011)
- Shrestha, J., "Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats", Indian J Pharmacol, 42, pp.306~311 (2010)
- Wang, C. C., "Areca-nut extract modulates antigen-specific immunity and augments inflammation in ovalbumin-sensitized mice", Immunopharmacol Immunotoxicol, 33, pp.315~322 (2011)
- Lee, K. K., "The effects of Areca catechu L extract on anti-aging", Int J Cosmet Sci, 21, pp.285~295 (1999)
- Wang, C. C., "Areca nut extract suppresses T-cell activation and interferon-gamma production via the induction of oxidative stress", Food Chem Toxicol, 45, pp.1410~1418 (2007)
- Chandra, P. S., "Prevalence and correlates of areca nut use among psychiatric patients in India", Drug Alcohol Depend, 69, pp.311~316 (2003)
- Gupta, P. C., "Oral submucosa fibrosis in india: a new epidemic?", Natl Med J India, 11, pp.113~116 (1998)
- Ho, P. S., "The incidence of oropharyngeal cancer in Taiwan: an endemic betel quid chewing area", J Oral Pathol Med, 31, pp.213~219 (2002)
- Kumar, M., "Effect of betel", areca nut (Areca catechu) extracts on intestinal epithelial cell lining, 2000, pp.42, 257~260
- 한국식품의약품안전청, "의약품 등의 독성시험기준", 한국식품의약품 안전청 고시 제 2009-116호, ~ (2009)
- Irwin, S., "Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse", Psychopharmacology, 13, pp.222~257 (1968)
- Dourish, C.T., "Effects of drugs on spontaneous motor activity. In Experimental Psychopharmacology", pp.325~344 (1987), Humana Press
- Plata, E. J., "Growth and haematologic properties of the BALB", wm strain of inbred mice, 1972, pp.22, 712~720
- Yamaguchi, C., "Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice", Exp Anim, 32, pp.1~11 (1983)
- JaeHyun Lee, "Single Subcutaneous Dose Toxicity of Polycan？, a β-Glucan Originated from Aureobasidium in Mice", Laboratory Animal Research, 21(4), pp.299~305 (2005), 한국실험동물학회
- Lee, H. S., "Single oral dose toxicity study of water extracts of Picrorrhiza Rhizoma in mice", J Toxicol Pub Health, 22, pp.117~126 (2006)
- Park, M. Y., "Single dose toxicity test of Kong-Jin-Dan, a polyherbal formula in ICR mice", J Appl Phramcol, 15, pp.245~251 (2007)
- Banks, W.J., "Lymphatic system and immunity. In: Banks WJ (Ed). Applied veterinary histology", pp.330~347 (1986), Williams & Wilkins
- Pineda, M.H., "Female reproductive system, In Veterinary endocrinology and reproduction", pp.303~354 (1989), Lea & Febiger
- US Environmental Protection Agency, "Health Effects Test Guidelines OPPTS 870.100, Acute Toxicity Testing Background" (1998), US EPA August