Single Oral Dose Toxicity Study of Water Extracts of Polygalae Radix in ICR Mice
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The objective of this study was to evaluate the single oral dose toxicity of Polygalae Radix (PR) in male and female mice. PR extract (yield = 18.6%) was administered to ICR mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines (2009-116, 2009). Animals were monitored for the mortality and the changes in body weight, clinical signs and gross observation during 14 days after dosing. Upon necropsy, organ weight and histopathology of 14 principal organs were examined. It was observed that there were no mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations against 14 principal organs related to PR extract up to 2,000 mg/kg. Therefore, 50% lethal dose (LD50) and approximate LD of PR aqueous extracts after single oral treatment in female and male mice were considered over 2000 mg/kg the limited dosages recommended by KFDA Guidelines, respectively.
Abstract
The objective of this study was to evaluate the single oral dose toxicity of Polygalae Radix (PR) in male and female mice. PR extract (yield = 18.6%) was administered to ICR mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines (2009-116, 2009). Animals were monitored for the mortality and the changes in body weight, clinical signs and gross observation during 14 days after dosing. Upon necropsy, organ weight and histopathology of 14 principal organs were examined. It was observed that there were no mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations against 14 principal organs related to PR extract up to 2,000 mg/kg. Therefore, 50% lethal dose (LD50) and approximate LD of PR aqueous extracts after single oral treatment in female and male mice were considered over 2000 mg/kg the limited dosages recommended by KFDA Guidelines, respectively.
참고문헌 (32)
- 이지은, "반하의 4주 반복투여독성 평가", Laboratory Animal Research, 19(3), pp.127~141 (2003), 한국실험동물학회
- 노성수, "Mouse Single Oral Dose Toxicity Study of DHU001, a Polyherbal Formula", 한국독성학회지, 26(1), pp.53~59 (2010), 한국독성학회
- Cheong, M. H., "Anti-inflammatory effects of Polygala tenuifolia root through inhibition of NF-B activation in lipopolysaccharide-induced BV2 microglial cells", J Ethnopharmacol, 137, pp.1402~1408 (2011)
- Choi, J. G., "Polygalae radix inhibits toxin-induced neuronal death in the Parkinson's disease models", J Ethnopharmacol, 134, pp.414~421 (2011)
- Liang, Z., "Neuroprotective effects of tenuigenin in a SH-SY5Y cell model with 6-OHDA-induced injury", Neurosci Lett, 497, pp.104~109 (2011)
- 이영종, "遠志가 NMDA로 유발된 신경세포 손상에 미치는 효과", 대한본초학회지, 20(2), pp.115~125 (2005), 대한본초학회
- Hu, Y., "Possible mechanism of the antidepressant effect of 3, 6'-disinapoyl sucrose from Polygala tenuifolia Willd", J Pharm Pharmacol, 63, pp.869~874 (2011)
- Hu, Y., "Antidepressant effects of the extract YZ-50 from Polygala tenuifolia in chronic mild stress treated rats and its possible mechanisms", Pharm Biol, 48, pp.794~800 (2010)
- Ikeya, Y., "Cognitive improving and cerebral protective effects of acylated oligosaccharides in Polygala tenuifolia", Biol Pharm Bull, 27, pp.1081~1085 (2004)
- Karakida, F., "Cerebral protective and cognition-improving effects of sinapic acid in rodents", Biol Pharm Bull, 30, pp.514~519 (2007)
- Xu, S. P., "Cognitive-enhancing effects of polygalasaponin hydrolysate in a(25-35)-induced amnesic mice", Evid Based Complement Alternat Med, 2011, pp.839~720 (2011)
- Kawashima, K., "Anti-stress effects of 3, 4, 5-trimethoxycinnamic acid, an active constituent of roots of Polygala tenuifolia(Onji)", Biol Pharm Bull, 27, pp.1317~1319 (2004)
- Hu, Y., "A bioactive compound from Polygala tenuifolia regulates efficiency of chronic stress on hypothalamic-pituitary-adrenal axis", Pharmazie, 64, pp.605~608 (2009)
- Yao, Y., "Anxiolytic and sedative-hypnotic activities of polygalasaponins from Polygala tenuifolia in mice", Pharm Biol, 48, pp.801~807 (2010)
- Lee, T.H., "The research on the pharmacological concept of sedative effect of Polygalae Radix", Kor. J. Herbology, 12(1), pp.1~6 (1997)
- 김경호, "Effects of Radix Polygalae Extract on Sociopsychological Stress", 대한본초학회지, 18(1), pp.12~108 (2003), 대한본초학회
- Park, H. J., "Effects of Polygala tenuifolia root extract on proliferation of neural stem cells in the hippocampal CA1 region", ytother Res, 22, pp.1324~1329 (2008)
- Spelman, K., "Modulation of cytokine expression by traditional medicines : a review of herbal immunomodulators", Altern Med Rev, 11, pp.128~150 (2006)
- Park, J. H., "Effect of Polygala tenuifolia root extract on cerebral ischemia and reperfusion", Am J Chin Med, 34, pp.115~123 (2006)
- Zhu, Y.P., "Chinese Materia Medica Chemistry. Pharmacology and Applications", pp.516~519 (1998), Harwood Academic Publishers
- Kim You Young, "Occupational Asthma and Rhinitis Induced by a Herbal Medicine, Wonji (Polygala tenuifolia)", Journal of Korean Medical Science, 20(1), pp.46~ (2005), 대한의학회
- Korea Food and Drug Administration, "Testing Guidelines for Safety Evaluation of Drugs" (2009), the Korea Food and Drug Administration
- Irwin, S., "Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse", Psychopharmacology, 13, pp.222~257 (1968)
- Dourish, C.T., "Effects of drugs on spontaneous motor activity in Experimental psychopharmacology", pp.325~334 (1987), Humana Press
- Levene, A., "Pathological factors influencing excision of tumours in the head and neck. Part I", Clin Otalary, 6, pp.145~151 (1981)
- Ludbrook, J., "Update: microcomputer statistics packages. A personal view", Clin Exp Pharmacol Physiol, 24, pp.294~296 (1997)
- Won-Ho Lee, "Single Oral Dose Toxicity Test of Platycodin D, a Saponin from Platycodin Radix in Mice", 한국독성학회지, 27(4), pp.217~224 (2011), 한국독성학회
- Flecknell, P., "Laboratory Animal Anesthesia", pp.269~ (1996), Harcourt Brace & Company
- Plata, E. J., "Growth and haematologic properties of the BALB", wm strain of inbred mice, 1972, pp.22, 712~720
- Yamaguchi, C., "Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice", Exp Anim, 32, pp.1~11 (1983)
- Banks, W.J., "Female reproductive system in Applied veterinary histology", pp.506~526 (1986), Williams & Wilkins
- Pineda, M.H., "Female reproductive system in Veterinary endocrinology and reproduction", pp.303~354 (1989), Lea & Febiger