본문 바로가기
KERD
논문KCI 등재

발효홍삼의 광노화 피부 억제효과에 대한 형태학적 연구

Morphological Studies on the Inhibitory Effects of Photoaging Skin of Fermented Red Ginseng in Hairless Mice

1

조회

0

다운로드

7

인용

0

참고문헌

키워드

주제분류한의학

초록

To investigate the anti-photoaging effect of fermented Red Ginseng(RG) in SKH-1 mice. We examined the effects of extracts of non-fermented RG(NRG group), fermented RG(FRG group) and fortified fermented RG(FFRG group) on skin wrinkles formation, histological changes related to the number of epidermal cell layers, epidermal thickness, neutrophil infiltration into dermis, degradation of collagen fibers, and the number of mast cells, and immunohistochemical changes related to cytokines and enzymes in photoaging skin caused by UVB irradiation of SKH-1 mice. The oral administration(300 mg/Kg B.W./day) and topical application(100 ㎕/mouse/day) of extracts of NRG, FRG and FFRG inhibited increases in epidermal thickness and wrinkle formation compared to control group in dorsal skin induced by UVB irradiation. We observed more increased stainability of acid fuschin and aniline blue in dermis of FFRG group than those of other groups. Furthermore, NRG, FRG and FFRG prevented the disruption of collagen fibers within papillary layer of dermis, and decreased number of mast cells in the dorsal skins induced by UVB irradiation. We observed fine wrinkle formation in FFRG group. Treatment with NRG, FRG and FFRG decreased immunohistochemical density of myeloperoxidase related to inflammation in the photoaging skin. We observed more decreased immunohistochemical density of myeloperoxidase in FFRG group than those of other groups. Immunohistochemical density of PCNA and Ki-67 in FFRG group was more decreased than those of other groups. Our study suggests that fermented red ginseng extracts participates in inhibitory effects in the morphological processes related to photoaging skin on UVB irradiated SKH-1 mice.

Abstract

To investigate the anti-photoaging effect of fermented Red Ginseng(RG) in SKH-1 mice. We examined the effects of extracts of non-fermented RG(NRG group), fermented RG(FRG group) and fortified fermented RG(FFRG group) on skin wrinkles formation, histological changes related to the number of epidermal cell layers, epidermal thickness, neutrophil infiltration into dermis, degradation of collagen fibers, and the number of mast cells, and immunohistochemical changes related to cytokines and enzymes in photoaging skin caused by UVB irradiation of SKH-1 mice. The oral administration(300 mg/Kg B.W./day) and topical application(100 ㎕/mouse/day) of extracts of NRG, FRG and FFRG inhibited increases in epidermal thickness and wrinkle formation compared to control group in dorsal skin induced by UVB irradiation. We observed more increased stainability of acid fuschin and aniline blue in dermis of FFRG group than those of other groups. Furthermore, NRG, FRG and FFRG prevented the disruption of collagen fibers within papillary layer of dermis, and decreased number of mast cells in the dorsal skins induced by UVB irradiation. We observed fine wrinkle formation in FFRG group. Treatment with NRG, FRG and FFRG decreased immunohistochemical density of myeloperoxidase related to inflammation in the photoaging skin. We observed more decreased immunohistochemical density of myeloperoxidase in FFRG group than those of other groups. Immunohistochemical density of PCNA and Ki-67 in FFRG group was more decreased than those of other groups. Our study suggests that fermented red ginseng extracts participates in inhibitory effects in the morphological processes related to photoaging skin on UVB irradiated SKH-1 mice.

참고문헌 (64)

  1. Meinhard, W., "Solar UV irradiation and dermal photoaging", J Photochem Photobiol, 63, pp.41~51 (2001)
  2. Lavker, R.M., "Structural alterations in exposed and unexposed aged skin", J Invest Dermatol, 73, pp.59~66 (1979)
  3. Gilchrest, B.A., "Skin aging and photoaging: an overview", J Am Acad Dermatol, 989(21), pp.610~613 (1989)
  4. Griffiths, C.E.M., "The clinical identification and quantification of photodamage", Br J Dermatol, 127(41), pp.37~42 (1992)
  5. Kligman, A. M., "Topical tretinoin for photoaged skin", J Am Acad Dermatol, 15, pp.836~859 (1986)
  6. Chung, J. H., "Modulation of skin collagen metabolism in aged and photoaged human skin in vivo", J Invest Dermatol, 117, pp.1218~1224 (2001)
  7. Gilchrest, B.A., "A review of skin ageing and its medical therapy", Br J Dermatol, 135, pp.867~875 (1996)
  8. Quan, T., "Matrix-degrading Metalloproteinases in Photoaging", J Investig Dermatol Symp Proc, 14(1), pp.20~24 (2009)
  9. 박창민, "3-O-cetyl-L-ascorbic acid의 주름 개선 효과", 대한화장품학회지, 34(4), pp.303~309 (2008), 대한화장품학회
  10. Wyss, R., "Chromatographic and electrophoretic analysis of biomedically important retinoids", J Chromatogr B, Biomed Sci, 671, pp.385~425 (1995)
  11. Weiss, J.S., "Topical tretinoin in the treatment of aging skin", J Am Acad Dermatol, 19, pp.169~175 (1988)
  12. Gary, J. F., "Mechanisms of photoaging and chronological skin aging", Arch Dermatol, 138, pp.1462~1470 (2002)
  13. Kim, Y. G., "Effects of ginseng saponins isolated from red ginseng on ultraviolet B-induced skin aging in hairless mice", Eur J Pharm, 602, pp.148~156 (2009)
  14. Sang-Jin Lee, "Inhibitory Effects of Ethanol Extracts from Nuruk on Oxidative Stress, Melanogenesis, and Photo-Aging", Mycobiology, 40(2), pp.117~123 (2012), 한국균학회
  15. 김명자, "홍삼식이가 여성의 목 피부 홍반과 탄력 개선에 미치는 효과", 한국미용학회지, 18(3), pp.661~669 (2012), 한국미용학회[DOI]
  16. 최윤경, "발효한약의 최근 연구 동향 - 안전성과 유효성 기반", 생명과학회지, 22(12), pp.1729~1739 (2012), 한국생명과학회
  17. 이지해, "Hairless mice에서의 대두 홍삼 혼합 분말(APB-03)의 경구 반복 투여 시 피부 주름 생성 예방 효과", 한국식품과학회지, 37(6), pp.989~996 (2005), 한국식품과학회
  18. Kang, T. H., "Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice", J Ethnopharmacol, 123, pp.446~451 (2009)
  19. Min Jung Lee, "Oral Administration of KTNG0345 Prepared from Red Ginseng Extracts Reduces UVB-induced Skin Wrinkle Formation in Hairless Mice", Journal of Ginseng Research, 32(1), pp.48~56 (2008), 고려인삼학회
  20. 정동원, "인삼, 홍삼 및 발효 홍삼이 정상인의 뇌혈류, 평균혈압, 맥박수에 미치는 영향", 대한한의학회지, 27(3), pp.37~49 (2006), 대한한의학회
  21. Bancroft, J. D., "Theory and practice of histological techniques", pp.127~128 (1996), Churchil Livingstone
  22. Hsu, S. M., "Use of avidin- biotin-peroxidase complex(ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody(PAP) procedures", J Histochem Cytochem, 29, pp.577~580 (1981)
  23. Placzec, M., "Tobacco smoke is phototoxic", Br J Dermatol, 150, pp.991~993 (2004)
  24. Fisher, G. J., "Mechanisms of photoaging and chronological skin aging", Arch Dermatol, 138, pp.1462~1470 (2002)
  25. Landau, M., "Exogenous factors in skin aging", Curr Probl Dermatol, 35, pp.1~13 (2007)
  26. Berneburg, M., "Photoaging of human skin", Photodermatol Photoimmunol Photomed, 16, pp.239~244 (2000)
  27. Sander, C. S., "Photoaging is associated with protein oxidation in human skin in vivo", J Invest Dermatol, 118(4), pp.618~625 (2002)
  28. Kiss, I., "The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling", Photochem Photobiol, 53, pp.109~112 (1991)
  29. 김동현, "인삼과 건강" (2005), 효일
  30. 안덕균, "기미론에서 본 인삼의 효능", 본초분과학회지, 7(1), pp.27~32 (1992)
  31. 허준, "동의보감. 탕액편", ~
  32. 노승현, "인삼의 효능", 본초분과학회지, 7(1), pp.53~60 (1992)
  33. 김창민, "중약대사전" (1997), 정담
  34. 주영승, "운곡본초학" (2004), 서림재
  35. 김은혜, "고려인삼이 항스트레스에 미치는 영향", 식품저장과 가공산업, 8(2), pp.13~18 (2009)
  36. 김대한, "人蔘 saponin 腸內 Glc-PPT 및 PPT가 免疫系에 미치는 影響에 關한 硏究", 대한본초학회지, 19(4), pp.105~116 (2004), 대한본초학회
  37. 이인선, "Streptozotocin-유도 당뇨모델을 이용한 배양인삼 분말의 혈당 강하 및 지질대사의 개선효과", 한국식품과학회지, 35(2), pp.280~285 (2003), 한국식품과학회
  38. 임창진, "흰쥐의 혈청 콜레스테롤 및 중성지방 수준에 미치는 인삼 총 사포닌의 영향", 한국생화학회지, 14(3), pp.188~198 (1981)
  39. 정영호, "콜레스티폴과 인삼사포닌이 생쥐의 콜레스테롤 대사에 미치는 영향", 한국지질학회지, 2(1), pp.16~23 (1992)
  40. 이산명, "녹용 및 목향수침이 흰쥐의 체중 및 소화관 호르몬 분비에 미치는 영향", 대한침구학회지, 5(1), pp.1~13 (1988)
  41. 김낙두, "인삼성분의 혈압강하기전에 관한 연구", 생약학회지, 9(1), pp.41~47 (1978)
  42. 주일우, "홍삼이 정상 흰쥐의 혈압 및 대동맥 조직 소견 변화에 미치는 영향", Journal of Ginseng Research, 32(4), pp.324~331 (2008), 고려인삼학회
  43. 최형기, "남성 갱년기 성기능 장애에서 홍삼이 성기능과 혈중 지질농도에 미치는 효과", 고려인삼학회지, 20(2), pp.125~132 (1996)
  44. 공병만, "백삼, 발효인삼, 홍삼 농축액의 이화학적 특성", Journal of Ginseng Research, 32(3), pp.238~243 (2008), 고려인삼학회
  45. 신용서, "CKDHC 0801과 CKDHC 0802 균주를 이용한 홍삼발효", 한국식품조리과학회지, 26(4), pp.469~474 (2010), 한국식품조리과학회
  46. Odani, T., "Studies on the absorption, distribution, excretion and metabolism of ginseng saponins. II. The absorption, distribution and excretion of ginsenoside Rg1 in the rat", Chem Pharm Bull, 31, pp.292~298 (1983)
  47. Oikarinen, A., "Aging of the skin connective tissue: how to measure the biochemical and mechanical properties of aging dermis", Photodermatol Photoimmunol Photomed, 10, pp.47~52 (1994)
  48. Braverman, I.M., "Studies in cutaneous aging. I. The elastic fiber network", J Invest Dermatol, 78, pp.434~443 (1982)
  49. Uitto, J., "Connective tissue biochemistry of the aging dermis. Age-related alterations in collagen and elastin", Dermatol Clin, 4, pp.433~446 (1986)
  50. Varani, J., "Inhibition of type I procollagen synthesis by damaged collagen in photoaged skin and by collagenase-degraded collagen in vitro", Am J Pathol, 158, pp.931~942 (2001)
  51. Kim, Y. G., "Effects of red ginseng extract on ultraviolet B-irradiated skin change in C57BL mice", Phytother Res, 22, pp.1423~1427 (2008)
  52. Gilchrest, B. A., "The human sunburn reaction: histologic and biochemical studies", J Am Acad Dermatol, 5, pp.411~422 (1981)
  53. Hart, P.H., "Mast cells in UV-B-induced immunosuppression", J Photochem Photobiol B: Biol, 55, pp.81~87 (2000)
  54. Pentland, A. P., "Enhanced prostaglandin synthesis after ultraviolet injury is mediated by endogenous histamine stimulation. A mechanism for irradiation erythema", J Clin Invest, 86, pp.566~574 (1990)
  55. Hart, P.H., "Dermal mast cells determine susceptibility to ultraviolet B-induced systemic suppression of contact hypersensitivity responses in mice", J Exp Med, 187, pp.2045~2053 (1998)
  56. Walsh, L.J., "Ultraviolet B irradiation of skin induces mast cell degranulation and release of tumour necrosis factor-α", Immunol Cell Biol, 73, pp.226~233 (1995)
  57. Moodycliffe, A.M., "Role of tumour necrosis factor-α in ultraviolet B light-induced dendritic cell migration and suppression of contact hypersensitivity", Immunology, 81, pp.79~84 (1994)
  58. Laberge, S., "Histamine induced secretion of lymphocyte chemoattractant factor from CD8q T cells is independent of transcription and translation. Evidence for constitutive protein synthesis and storage", J Immunol, 155, pp.2902~2910 (1995)
  59. Lagier, B., "Different modulation by histamine of IL-4 and interferon-gamma (IFN-γ) release according to the phenotype of human Th0, Th1 and Th2 clones", Clin Exp Immunol, 108, pp.545~551 (1997)
  60. Chaloob, M.K., "Immunohistochemical expression of Ki-67, PCNA and CD34 in astrocytomas: A clinicopathological study", Oman Med J, 27(5), pp.368~374 (2012)
  61. Syed, D. N., "Photochemopreventive effect of pomegranate fruit extract on UVA-mediated activation of cellular pathways in normal human epidermal keratinocytes", Photochem Photobiol, 82(2), pp.398~405 (2006)
  62. Sundaresan, M., "Requirement for generation of H2O2 for platelet-derived growth factor signal transduction", Science, 270, pp.296~299 (1995)
  63. Lu, Y. P., "Time course for early adaptive responses to ultraviolet B light in the epidermis of SKH-1 mice", Cancer Res, 59, pp.4591~4602 (1999)
  64. Oberyszyn, T. M., "Inhibitory effects of pentoxifylline on ultraviolet B light-induced cutaneous inflammation", Mol Carcinog, 22, pp.16~258 (1998)

관련 논문AI추천

이 간행물의 다른 논문 (15건)