Protective Effect of Soybean-Derived Phosphatidylserine on the Trimethyltin-Induced Learning and Memory Deficits in Rats
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The present study examined the effects of soybean-derived phosphatidylserine (SB-PS) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The cognitive improving efficacy of SB-PS on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing cholineacetyl transferase (ChAT), acetylcholinesterase (AChE) and cAMP responsive element binding protein (CREB) immunohistochemistry. A positron emission tomography (PET) scanning the rat brain was by performed administer 18F-Fluorodeoxy-glucose (18F-FDG). The rats with TMT injection showed impaired learning and memory of the tasks and treatment with SB-PS produced a significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd day compared to that of the MCT group. In the retention test, the SB-PS group showed increased time spent around the platform compared to that of the MCT group. Consistent with the behavioral data, SB-PS 50 group significantly alleviated the loss of acetyl cholinergic neurons in the hippocampus compared to that of the MCT group. Treatment with SB-PS significantly increased the CREB positive neurons in the hippocampus as compared to that of the MCT group. In addition, SB-PS groups increased the glucose uptake in the hippocampus and SB-PS 50 group increased the glucose uptake in the frontal lobe, as compared to that of the MCT group. These results suggest that SB-PS may be useful for improving the cognitive function via regulation of cholinergic marker enzyme activity and neural activity.
Abstract
The present study examined the effects of soybean-derived phosphatidylserine (SB-PS) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The cognitive improving efficacy of SB-PS on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing cholineacetyl transferase (ChAT), acetylcholinesterase (AChE) and cAMP responsive element binding protein (CREB) immunohistochemistry. A positron emission tomography (PET) scanning the rat brain was by performed administer 18F-Fluorodeoxy-glucose (18F-FDG). The rats with TMT injection showed impaired learning and memory of the tasks and treatment with SB-PS produced a significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd day compared to that of the MCT group. In the retention test, the SB-PS group showed increased time spent around the platform compared to that of the MCT group. Consistent with the behavioral data, SB-PS 50 group significantly alleviated the loss of acetyl cholinergic neurons in the hippocampus compared to that of the MCT group. Treatment with SB-PS significantly increased the CREB positive neurons in the hippocampus as compared to that of the MCT group. In addition, SB-PS groups increased the glucose uptake in the hippocampus and SB-PS 50 group increased the glucose uptake in the frontal lobe, as compared to that of the MCT group. These results suggest that SB-PS may be useful for improving the cognitive function via regulation of cholinergic marker enzyme activity and neural activity.
참고문헌 (39)
- Stuchbury, G., "Alzheimer's associated inflammation, potential drug targets and future therapies", J. Neural. Transm., 112(3), pp.429~453 (2005)
- Cummings, J.L., "Treatment of Alzheimer's disease current and future therapeutic approaches", Rev Neurol Dis, 1, pp.60~69 (2004)
- Nordberg, A., "Cholinergic topography in Alzheimer brains a comparison with changes in the monoaminergic profile", J. Neural. Transm, 69, pp.19~32 (1987)
- acetylcholine binding sites in the frontal cortex of Alzheimer brains, "Neurosci. Lett", 1986, 115~119
- Wheeler, K. P., "ATPase activity of the sodium pump needs phosphatidylserine", Nature, 225, pp.449~450 (1970)
- Delwaide, P. J., "Double-blind randomized controlled study of phosphatidylserine in senile demented patients", Acta. Neurol. Scand, 73(2), pp.136~140 (1986)
- Prusiner, S.B., "Molecular biology of prion disease", Science, 252(5012), pp.1515~1522 (1991)
- Kudo, "Biosurfactants as food additives", Proceedings of the World Conference on Biotechnology for the Fats and Oils Industry, pp.195~201 (1987), American Oil Chemist's Society
- Blokland, A., "Cognition-enhancing properties of subchronic phsophatidylserine (PS) treatment in middle-aged rats comparison of bovine cortex PS with egg PS and soybean PS", Nutrition, 15(10), pp.778~783 (1999)
- Jorissen, B. l., "The influence of soy-derived phosphatdiylserine on cognition in age-associated memory impairment", Nutr. Neurosci, 4(2), pp.121~134 (2001)
- Sakai, M., "Pharmacological effects of phosphatidylserine enzymatically synthesized from soybean lecithin on brain functions in rodents", J. Nutr. Sci. Vitaminol, 42(1), pp.47~54 (1996)
- Cannon, R. L., "Effects of trimethyltin (TMT) on choline acetyltransferase activity in the rat hippocampus", Mol. Chem. Neuropathol, 23(1), pp.27~45 (1994)
- Cannon, R. L., "The effect of time following exposure to trimethyltin (TMT) on cholinergic muscarinic receptor binding in rat hippocampus", Mol. Chem. Neuropathol, 23(1), pp.47~62 (1994)
- O'connell, A., "The neuroprotective effect of tacrine on trimethyltin induced memory and muscarinic receptor dysfunction in the rat", Neurochem. Int, 25(6), pp.555~566 (1994)
- Brabeck, C., "Expression of EMAP-II by activated monocytes", microglia cells in different regions of the rat hippocampus after trimethyltin-induced brain damage, 2002, pp.177(1), 341~346
- Dyer, R.S., "Physiological methods for assessment of Trimethyltin exposure", Neurobehav. Toxicol. Teratol, 4(6), pp.659~664 (1982)
- Balaban, C. D., "Trimethyltin-induced neuronal damage in the rat brain comparative studies using silver degeneration stains, immunocytochemistry and immunoassay for neurotypic and gliotypic proteins", Neuroscience, 26(1), pp.337~361 (1988)
- Brown, A. W., "The behavioral and neuropathologic sequelae of intoxication by trimethyltin compounds in the rat", Am. J. Pathol, 97(1), pp.59~82 (1979)
- Swartzwelder, H. S., "Activity changes in rats following acute trimethyltin exposure", Neurotoxicology, 2(3), pp.589~593 (1981)
- Woodruff, M. L., "Effects of trimethyltin on acquisition and reversal of a light-dark discrimination by rats", Physiol Behav, 55(6), pp.1055~1061 (1994)
- Walsh, T. J., "Trimethyltin impairs retention of a passive avoidance task. Neurobahav", Toxicol. Teratol, 4(2), pp.163~167 (1982)
- Walsh, T. J., "Trimethyltin, a selective limbic system neurotoxicant, impairs radical-arm maze performance", Neurobahav. Toxicol. Teratol, 4(2), pp.177~183 (1982)
- Earley, B., "A comparison of the psychopharmacological profiles of phencyclidine, ketamine and (+)SKF 10,047 in the trimethyltin rat model", Neuropharmacology, 29(8), pp.695~703 (1990)
- Zhao, T. F., "Effect of Tremella fuciformis Berk on acute radiation sickness in dogs", Zhongguo. Yi. Xue. Ke. Xue. Yuan. Xue. Bao, 4(1), pp.20~23 (1982)
- Hooge, R., "Applications of the Morris water maze in the study of learning and memory", Brain. Res. Rev, 36(1), pp.60~90 (2001)
- Paxinos, G., "Bregma, lambda and the interaural midpoint in stereotaxic surgery with rats of different sex, strain and weight", J. Neurosci. Methods, 13(2), pp.139~143 (1985)
- Fueger, B. J., "Impact of animal handling on the results of 18F-FDG PET studies in mice", J. Nucl. Med, 47(6), pp.999~1006 (2006)
- Bao, Q., "Performance evaluation of the inveon dedicated PET preclinical tomograph based on the NEMA NU-4 standards", J. Nucl. Med, 50(3), pp.401~408 (2009)
- 유아람, "소동물 영상을 위한 Siemens Inveon PET 스캐너의 성능평가", 의학물리, 21(2), pp.145~152 (2010), 한국의학물리학회
- Kim, J. S., "Assessment of Cerebral Glucose Metabolism in Cat Deafness Model: Strategies for Improving the Voxel-Based Statistical Analysis for Animal PET Studies", Mol. Imaging Biol, 10(3), pp.154~161 (2008)
- Koczyk, D., "Trimethyltin-induced plastic neuronal changes in rat hippocampus are accompanied by astrocytic trophic activity", Acta. Neurobiol. Exp, 56(1), pp.237~241 (1996)
- Asano, T., "The effect of soybean derived phosphatidylserine on the cognitive function of the elderly", Jpn J. Nutr. Ass, 24(2), pp.165~170 (2005)
- Sun, H., "Effects of one Chinese herbs on improving cognitive function and memory of Alzheimer's disease mouse models", Zhongguo. Zhong. Yao. Za. Zhi, 28(8), pp.751~754 (2003)
- Hardinghan, G.E., "The Yin and Yang of NMDA receptor signaling", Trends Neurosci, 26(2), pp.81~89 (2003)
- Lonze, B. E., "Function and regulation of CREB family transcription factors in the nervous system", Neuron, 35(4), pp.371~385 (2002)
- Kim, J. H., "Effect of Tremella fuciformis on the neurite outgrowth of PC12h cells and the improvement of memory in rats", Biol. Pharm. Bull., 30(4), pp.708~714 (2007)
- Bourtchuladze, R., "Deficient long-term memory in mice with a targeted mutation of the cAMP-responsive element-binding protein", Cell, 79(1), pp.59~68 (1994)
- Jackson, T., "Prospects of memory- modifying drugs that target the CREB pathway", Curr. Opin. Discov. Devel, 6(5), pp.712~719 (2003)
- Mirrione, M. M., "PET imaging of glucose metabolism in a mouse model of temporal lobe epilepsy", Synapse, 59(2), pp.119~121 (2006)