Trimethyltin으로 유도된 기억장애 흰쥐에서 백삼의 신경보호효과
The Neuroprotective Effect of White Ginseng (Panax ginseng C. A. Meyer) on the Trimethyltin (TMT)-Induced Memory Deficit Rats
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The present study examined the effects of Korean white ginseng (WG, Panax ginseng C. A. Meyer) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The rats were administered with saline or WG (WG 100 or 300 ㎎/㎏, p.o.) daily for 21 days. The cognitive improving efficacy of WG on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing immunohistochemistries on choline acetyltransferase (ChAT), acetylcholinesterase (AchE), cAMP responsive element binding protein (CREB) and brain derived neurotrophic factor (BDNF). The rats treated with TMT injection (control group) showed impaired learning and memory of the tasks, but the rats treated with TMT injection and WG administration produced significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd and 4th days compared to that of the control group. In the retention test, the WG 100 and WG 300 groups showed significantly increased crossing number around the platform compared to that of the control group (p < 0.001). Consistently with the behavioral data, result of immunohistochemistry analysis showed that WG 100 ㎎/㎏ significantly alleviated the loss of BDNF-ir neurons in the hippocampus compared to that of the control group (p < 0.01). Also, treatment with WG has a trend to be increased the cholinergic neurons in the hippocampal CA1 and CA3 areas as compared to that of the control group. These results suggest that WG may be useful for improving the cognitive function via regulation of neurotrophic activity.
Abstract
The present study examined the effects of Korean white ginseng (WG, Panax ginseng C. A. Meyer) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The rats were administered with saline or WG (WG 100 or 300 ㎎/㎏, p.o.) daily for 21 days. The cognitive improving efficacy of WG on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing immunohistochemistries on choline acetyltransferase (ChAT), acetylcholinesterase (AchE), cAMP responsive element binding protein (CREB) and brain derived neurotrophic factor (BDNF). The rats treated with TMT injection (control group) showed impaired learning and memory of the tasks, but the rats treated with TMT injection and WG administration produced significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd and 4th days compared to that of the control group. In the retention test, the WG 100 and WG 300 groups showed significantly increased crossing number around the platform compared to that of the control group (p < 0.001). Consistently with the behavioral data, result of immunohistochemistry analysis showed that WG 100 ㎎/㎏ significantly alleviated the loss of BDNF-ir neurons in the hippocampus compared to that of the control group (p < 0.01). Also, treatment with WG has a trend to be increased the cholinergic neurons in the hippocampal CA1 and CA3 areas as compared to that of the control group. These results suggest that WG may be useful for improving the cognitive function via regulation of neurotrophic activity.
참고문헌 (29)
- Balaban CD, "Trimethyltin-induced neuronal damage in the rat brain: comparative studies using silver degeneration strains, immunocytochemistry and immune-assay for neuronotypic and gliotypic proteins", Neuroscience, 26, pp.337~361 (1988)
- Balin B, "Toward a unifying hypothesis in the devleopment of Alzheimer's disease", CNS Neuroscience &Therapeutics, 17, pp.587~589 (2011)
- Castren E, "Brain-derived neurotrophic factor messenger RNA is expressed in the septum, hypothalamus and in adrenergic brain stem nuclei of adult rat brain and is increased by osmotic stimulation in the paraventricular nucleus", Neuroscience, 64, pp.71~80 (1995)
- Chang LW, "A time-course study of trimethyltin induced neuropathology in rats", Neurobehavioral Toxicology and Teratology, 5, pp.43~49 (1983)
- Chang LW, "Septotemporal gradients of trimethyltin-induced hippocampal lesions", Neurobehavioral Toxicology and Teratology, 7, pp.43~49 (1985)
- Chang LW, "Trimethyltin induced pathology in sensory neurons", Neurobehavioral Toxicology and Teratology, 5, pp.673~696 (1985)
- Chang LW, "Species and strain comparison of acute neurotoxic effects of trimethyltin in mice and rats", Neurobehavioral Toxicology and Teratology, 5, pp.337~350 (1985)
- Chang LW, "Neuropathology of trimethyltin intoxication. III. Changes in the brain stem neurons", Environmental Research, 30, pp.399~411 (1985)
- 최재을, "인삼의 가공방법에 따른 일반성분 및 Ginsenoside 함량 변화", 한국약용작물학회지, 18(2), pp.118~125 (2010), 한국약용작물학회
- 최재호, "백삼 및 홍삼 추출물의 사상체질별 면역세포 활성효과", Journal of Ginseng Research, 33(1), pp.33~39 (2009), 고려인삼학회
- Davinelli S, "The "Alzheimer's disease signature": potential perspectives for novel biomarkers", Immunity & Ageing, 8, pp.7~12 (2011)
- Dyer RS, "Physiological methods for assessment of trimethyltin exposure", Neurobehavioral Toxicology and Teratology, 4, pp.659~664 (1982)
- Fortemps E, "Trimethyltin poisoning: report of two cases", Environmental Health : A Global Access Science Source, 41, pp.1~6 (1978)
- Gray SG, "Epigenetic treatment of neurological disease", Epigenomics, 3, pp.431~450 (2011)
- Hamilton DA, "Control of rodent and human spatial navigation by room and apparatus cues", Behavioral Processes, 81, pp.154~169 (2009)
- 황은영, "백삼과 홍삼의 페놀성 성분 함량 및 멜라닌 생성억제효과", Journal of Ginseng Research, 30(2), pp.82~87 (2006), 고려인삼학회
- Ishida N, "Trimethyltin syndrome as a hippocampal degeneration model:temporal changes and neurochemical features of seizure susceptibility and learning impairment", Neuroscience, 81, pp.1183~1191 (1997)
- Seong Soo Joo, "Ginsenoside Rg3 from Red Ginseng Prevents Damage of Neuronal Cellsthrough the Phosphorylation of the Cell Survival Protein Akt", Food Science and Biotechnology, 15(2), pp.244~247 (2006), 한국식품과학회
- Kang H, "requirement for local protein synthesis in neurotrophin-induced hippocampal synaptic plasticity", Science, 274, pp.1402~1406 (1996)
- Karege F, "Low brain-derived neurotrophic factor (BDNF) levels in serum of depressed patients probably results from lowered platelet BDNF release unrelated to platelet activity", Biological Psychiatry, 57, pp.1068~1072 (2005)
- 김금숙, "수경재배 인삼의 부위별 진세노사이드 분석", 원예과학기술지, 28(2), pp.216~226 (2010), 한국원예학회
- Koczyk D, "How does trimethyltin affect the brain: facts and hypotheses", Acta Neurobiologiae Experimentalis, 56, pp.587~96 (1996)
- 이해준, "방사선 조사 마우스에서 학습기억 장애에 대한 홍삼의 효과", Journal of Ginseng Research, 33(2), pp.132~138 (2009), 고려인삼학회
- Liu XL, "The effect of dietary Panax ginseng polysaccharide extract on the immune responses in white shrimp, Litopenaeus vannamei", Fish Shelfish Immunology, 30, pp.495~500 (2011)
- Park JD, "Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C.A. Meyer)", Journal of Ginseng Research, 30, pp.389~415 (1996)
- Paxinos G, "The rat brain in stereotaxic coordinates. (2nd ed)", pp.1~59 (1986), Academic Press
- Radka SF, "Presence of brain derived neurotrophic factor in brain and human and rat but not mouse serum detected by a sensitive and specific immunoassay", Brain Research, 709, pp.122~130 (1996)
- Robins Wahlin TB, "Personality changes in Alzheimer's disease: a systematic review", International Journal of Geriatric Psychiatry, 26, pp.1019~1029 (2010)
- Stewart S, "Which memory task for my mouse? A systematic review of spatial memory performance in the tg2576 Alzheimer's mouse model", Journal of Alzheimer's Disease, 26, pp.105~126 (2011)