당귀 추출물의 궤양성 대장염 억제 효과에 대한 연구
Inhibitory Effects of Angelica gigas Nakai on Ulcerative Colitis in DSS-induced ICR Mice
1
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
The purpose of this study was to examine the effects of Angelica gigas Nakai on ulcerative colitis. Mice were divided into 5 groups: Control group, DSS group, 5-ASA group, 50mg/kg Angelica gigas Nakai group, 100mg/kg Angelica gigas Nakai group. Four groups, excluding the control group, were fed a 5% solution of dextran sulfate sodium(DSS) in water for 7days to induce ulcerative colitis. Each water extract was administrated orally for 7 days in 5-ASA group, 50mg/kg Angelica gigas Nakai group and 100mg/kg Angelica gigas Nakai group. 5 groups were evaluated by weight, length of intestine, weight of spleen, disease activity index(DAI), amount of cytokine IL-6 production, thickness of bowel wall and degree of inflammatory cell infiltration and intestinal tissue damage. Comparing to DSS group, 100 mg/kg Angelica gigas Nakai group showed significant suppressive effect of weight loss until 4th day of experiment while 50 mg/kg Angelica gigas Nakai group showed no significant effect of suppression. Decrease of intestinal length, enlargement of spleen, intestinal tissue damage and thickening of bowel wall were significantly suppressed in both 50 mg/kg and 100mg/kg Angelica gigas Nakai group. Also disease activity and cytokine IL-6 production were inhibited significantly. Based on this result, Angelica gigas Nakai seemed to have anti-inflammatory effect and also seemed to suppress histological changes and aggravation of ulcerative colitis.
Abstract
The purpose of this study was to examine the effects of Angelica gigas Nakai on ulcerative colitis. Mice were divided into 5 groups: Control group, DSS group, 5-ASA group, 50mg/kg Angelica gigas Nakai group, 100mg/kg Angelica gigas Nakai group. Four groups, excluding the control group, were fed a 5% solution of dextran sulfate sodium(DSS) in water for 7days to induce ulcerative colitis. Each water extract was administrated orally for 7 days in 5-ASA group, 50mg/kg Angelica gigas Nakai group and 100mg/kg Angelica gigas Nakai group. 5 groups were evaluated by weight, length of intestine, weight of spleen, disease activity index(DAI), amount of cytokine IL-6 production, thickness of bowel wall and degree of inflammatory cell infiltration and intestinal tissue damage. Comparing to DSS group, 100 mg/kg Angelica gigas Nakai group showed significant suppressive effect of weight loss until 4th day of experiment while 50 mg/kg Angelica gigas Nakai group showed no significant effect of suppression. Decrease of intestinal length, enlargement of spleen, intestinal tissue damage and thickening of bowel wall were significantly suppressed in both 50 mg/kg and 100mg/kg Angelica gigas Nakai group. Also disease activity and cytokine IL-6 production were inhibited significantly. Based on this result, Angelica gigas Nakai seemed to have anti-inflammatory effect and also seemed to suppress histological changes and aggravation of ulcerative colitis.
참고문헌 (25)
- Owczarek, D., "Biological therapy of inflammatory bowel disease", Pol Arch Med Wewn, 119(1-2), pp.84~88 (2009)
- Akiyama, S., "Effects of anthocyanin-rich tea ‘‘Sunrouge’’ on dextran sodium sulfate-induced colitis in mice", Biofactors, 38(3), pp.226~233 (2012)
- Hanauer, S. B., "Inflammatory bowel disease", N Engl J Med, 334(13), pp.841~848 (1996)
- Xu, C. T., "Drug therapy for ulcerative colitis", World J Gastroenterol, 10(16), pp.2311~2317 (2004)
- College of Oriental medicine, department of internal medicine, "The internal medicine", pp.374~375 (2012), koonja publisher
- Ardizzone, S., "Biologic therapy for inflammatory bowel disease", Drugs, 65(16), pp.2253~2286 (2005)
- Heo jun, "Donguibogam", pp.2169~2170 (2010), Donguibogam publisher
- Wang, X., "Effects of an angelica extract on human erythrocyte aggregation, deformation and osmotic fragility", Clin Hemorheol Microcirc, 24(3), pp.201~205 (2001)
- Zhui, Y., "Experimental study of the antiatherogenesis effect of Chinese medicine angelica and its mechanisms", Clin Hemorheol Microcirc, 22(4), pp.305~310 (2000)
- Chawla, A. S., "Anti-inflammatory action of ferulic acid and its esters in carrageenan induced rat paw oedema model", Indian J Exp Biol, 25(3), pp.187~189 (1987)
- Jang, S. I., "Anti-Inflammatory Effect of Ethanol Extract of Angelica uchiyamana in Activated Murine RAW 264.7 macrophages", Herbal formula science, 10(2), pp.189~197 (2002)
- Shin, J. S., "Monotropein isolated from the roots of Morinda officinalis ameliorates proinflammatory mediators in RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis via NF-kB inactivation", Food Chem Toxicol, 53(3), pp.263~271 (2013)
- Peyrin-Biroulet, L., "Crohn’s disease: beyond antagonists of tumour necrosis factor", Lancet, 372(9632), pp.67~81 (2008)
- Brown, W. A., "5-Aminosalicyclic acid and olsalazine inhibit tumor growth in a rodent model of colorectal cancer", Digestive Diseases and Sciences, 45(8), pp.1578~1584 (2000)
- Fiocchi, C., "Inflammatory bowel disease: etiology and pathogenesis", Gastroenterology, 115(1), pp.182~205 (1998)
- College of Oriental medicine Department of Herbology, "The Herbology", pp.578~579 (2008), Yeongnimsa;
- Heo jun, "Donguibogam", pp.854~855 (2010), Donguibogam publisher
- Heo jun, "Donguibogam", pp.414~415 (2010), Donguibogam publisher
- Wirtz, S., "Mouse models of inflammatory bowel disease", Adv Drug Delivery Rev, 59(11), pp.1073~1083 (2007)
- Okayasu, I., "A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice", Gastroenterology, 98(3), pp.694~702 (1990)
- Cooper, H. S., "Clinicopathologic study of dextran sulfate sodium experimental murine colitis", Lab. Invest, 69(2), pp.238~249 (1993)
- Ni, J., "Effects of dextran sulphate sodium on intestinal epithelial cells and intestinal lymphocytes", Gut, 39(2), pp.234~241 (1996)
- Takizawa, H., "Immunological studies of the mucosa in colitis induced by sodium dextran sulfate in rats", Adv Exp Med Biol, 371B, pp.1383~1387 (1995)
- Takizawa, H., "Activated immunocompetent cells in rat colitis mucosa induced by dextran sulfate sodium and not complete but partial suppression of colitis by FK506", Digestion, 56(3), pp.259~264 (1995)
- Dieleman, L. A., "Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines", Clin Exp Immunol, 114(3), pp.385~391 (1998)