열처리된 무 추출물의 궤양성 대장염 예방 및 항염증 효과에 미치는 영향
Effect of Heated Radish Extract on DSS-Induced Ulcerative Colitis and Anti-Inflammatory Effect
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This study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and its combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): I-normal group, II-DSS control group, III-DSS + sulfasalazine (30 mg/kg) group, IV-DSS + sulfasalazine (60 mg/kg) group, and V-DSS + sulfasalazine (30 mg/kg) + radish extract mixture (30 mg /kg) (SRE) group. DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length, and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through the suppression of both MAPK and nuclear factor-kappa B (NF- B) signaling pathways and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that radish extract mixture combined with sulfasalazine protects one against experimental UC via the inhibition of both inflammation and apoptosis, which is highly similar to the standard-of-care sulfasalazin.
Abstract
This study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and its combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): I-normal group, II-DSS control group, III-DSS + sulfasalazine (30 mg/kg) group, IV-DSS + sulfasalazine (60 mg/kg) group, and V-DSS + sulfasalazine (30 mg/kg) + radish extract mixture (30 mg /kg) (SRE) group. DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length, and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through the suppression of both MAPK and nuclear factor-kappa B (NF- B) signaling pathways and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that radish extract mixture combined with sulfasalazine protects one against experimental UC via the inhibition of both inflammation and apoptosis, which is highly similar to the standard-of-care sulfasalazin.
참고문헌 (17)
- 정준석, "Real-time Intelligent Exit Path Indicator Using BLE Beacon Enabled Emergency Exit Sign Controller", The International Journal of Advanced Smart Convergence, 6(1), pp.82~88 (2017), 한국인터넷방송통신학회[DOI]
- 전형준, "A Study on the Quality of Photometric Scanning Under Variable Illumination Conditions", The International Journal of Advanced Smart Convergence, 6(4), pp.88~95 (2017), 한국인터넷방송통신학회[DOI]
- 김현경, "Physiological Functionalities and Anti-oxidant activity of heated radish extract", The International Journal of Advanced Smart Convergence, 7(2), pp.38~46 (2018), 한국인터넷방송통신학회[DOI]
- ShailubhaiK, V., "Plecanatide and dolcanatide, novel guanylate cyclase-Cagonists, ameliorate gastrointestinal inflammation in experimental models of murine colitis", World journal of gastrointestinal pharmacology and therapeutics, 6(4), pp.213~222 (2015)
- Kaser, A., "Inflamm atory bowel disease", Annual review of immunology, 28, pp.573~621 (2010)
- Ordás, I., "Ulcerative colitis", The Lancet, 380(9853), pp.1606~1619 (2012)
- Ross, A. S., "Medical therapy for ulcerative colitis: the state of the art and beyond", Current gastroenterology reports, 6(6), pp.488~495 (2004)
- Xiao, H. T., "Qing-dai powder promotes recovery of colitis by inhibiting inflammatory responses of colonic macrophages in dextran sulfate sodium-treated mice", Chinese medicine, 13(10), pp.29~34 (2015)
- Wong, V. W., "A hospital clinic-based survey on traditional Chinese medicine usage among chronic hepatitis B patients", Complementary therapies in medicine, 13(3), pp.175~182 (2005)
- Hattori, T., "Studies on antinephritic effect of TJ-8014, a new Japanese herbal medicine, and its mechanisms (1): Effects on original-type anti-GBM nephritis in rats and pl atelet aggregation", Japanese journal of pharmacology, 52(1), pp.477~485 (1990)
- Sanchez-Munoz, F., "Role of cytokines in inflammat ory bowel disease", World journal of gastroenterology, 14(27), pp.4280~4288 (2008)
- Jin, B. R,., "Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice viadual inhibition of NF-κB and STAT3 activation", Scientific reports, 7, pp.46252~ (2017)
- Rogler, G., "Gastrointestinal and liver adverse effects of drugs used for treating IBD", Best practice & research. Clinical gastroenterology, 24(2), pp.157~165 (2010)
- Buchheister, S., "CD14 Plays a Protective Role in Experimental Inflammatory Bowel Disease by Enhancing Intestinal Barrier Function", The American journal of pathology, 187(5), pp.1106~1120 (2017)
- Pandurangan, A. K., "Allicin Alleviates Dextran Sodium Sulfate-(DSS-) Induced Ulcerative Colitis in BALB", c Mice, 2015, pp.2015, 605208~
- Wei, D. D., "Apocynin relieves inflammation in dextran sulfate sodiu m-induced ulcerative colitis mice: the role of NOXs-ROS-p38MAPK pathway", Sheng Li Xue Bao, 67(1), pp.74~82 (2015)
- Islam, M., "Anti-inflammatory effects of phytosteryl ferulates in col itis induced by dextran sulphate sodium in mice", British Journal of Pharmacology, 154(4), pp.812~824 (2008)