본문 바로가기
KERD
논문KCI 등재

消炎整腸湯이 DSS로 誘發된 생쥐의 궤양성 대장염에 미치는 影響

The Effects of Soyumjungjang-tang on DSS-Induced Ulcerative Colitis in Mouse

윤성우|류봉하|송영근
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2008.06|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제29권 제2호|pp. 385-400

1

조회

0

다운로드

14

인용

0

참고문헌

키워드

IL-6COX-2IL-1βUlcerative ColitisSoyumjungjang-tangIκB
주제분류한의학

초록

Objectives : This study was carried out to investigate the effects of Soyumjungjang-tang(SJT) on the experimental ulcerative colitis induced by dextran sulfate sodium(DSS) in mice.Methods : Ulcerative colitis was induced through supplying 4% DSS solution as the drinking water for 7 days in 6-week-old male ICR mice. The colitic mice were divided into three groups: the sample groups were orally administered SJT in doses of 25mg/kg(S25 group) or 100mg/kg(S100 group) once a day for 10 days, from 3 days before starting drinking the DSS solution, and the control(C) group was administered normal saline instead of SJT. The DSS solution or SJT was not administered to the normal(N) group. The length of colon, histologic finding, the activities of myeloperoxidase(MPO) and alkaline phosphatase(AP), and the expressions of IL-1β, IL-6, COX-2, NF-κB, and IκB in colonic mucosa was checked using immunoblot, ELISA, etc. The activities of chondroitinase, tryptophanase, β-glucuronidase and β-glucosidase in stool were also measured.Results : The length of colon shortened, histologic finding deteriorated, the activities of MPO, AP, chondroitinase, tryptophanase, β-glucuronidase and β-glucosidase, and the expressions of IL-1β, IL-6, COX-2, NF-κB increased, and the expression of I-κB decreased in the C group. All measures, except NF-κB, were restored in S25 group, but some measures deteriorated more in the S100 group than in the C group. Conclusions : According to the above results, it is supposed that SJT has a potential therapeutic effect on ulcerative colitis.

Abstract

Objectives : This study was carried out to investigate the effects of Soyumjungjang-tang(SJT) on the experimental ulcerative colitis induced by dextran sulfate sodium(DSS) in mice.Methods : Ulcerative colitis was induced through supplying 4% DSS solution as the drinking water for 7 days in 6-week-old male ICR mice. The colitic mice were divided into three groups: the sample groups were orally administered SJT in doses of 25mg/kg(S25 group) or 100mg/kg(S100 group) once a day for 10 days, from 3 days before starting drinking the DSS solution, and the control(C) group was administered normal saline instead of SJT. The DSS solution or SJT was not administered to the normal(N) group. The length of colon, histologic finding, the activities of myeloperoxidase(MPO) and alkaline phosphatase(AP), and the expressions of IL-1β, IL-6, COX-2, NF-κB, and IκB in colonic mucosa was checked using immunoblot, ELISA, etc. The activities of chondroitinase, tryptophanase, β-glucuronidase and β-glucosidase in stool were also measured.Results : The length of colon shortened, histologic finding deteriorated, the activities of MPO, AP, chondroitinase, tryptophanase, β-glucuronidase and β-glucosidase, and the expressions of IL-1β, IL-6, COX-2, NF-κB increased, and the expression of I-κB decreased in the C group. All measures, except NF-κB, were restored in S25 group, but some measures deteriorated more in the S100 group than in the C group. Conclusions : According to the above results, it is supposed that SJT has a potential therapeutic effect on ulcerative colitis.

참고문헌 (40)

  1. 한동수, "다량의 복수가 주 증상으로 나타난 POEMS 증후군 1예", 대한소화기학회지, 40(3), pp.1~1 (2002), 대한소화기학회
  2. Satoshi M, "Enteric bacteria and their roles in inflmmatory bowel disease", Bioscience Microflora, 18(1), pp.1~9 (1999)
  3. 전국의과대학교수, "오늘의 診斷 및 治療", pp.691~695 (2000), 한우리
  4. Hanauer SB, "Medical therapy for ulcerative colitis", Gastroenterology, 126, pp.1582~1592 (2004)
  5. 許浚, "東醫寶鑑", pp.183~ (1994), 南山堂
  6. 윤상협, "補腸健脾湯加減方으로 膠原性 大腸炎의 15年 泄瀉를 好轉시켰던 治驗 1例", 成人病學會誌, 7(1), pp.78~81 (2001)
  7. 서철훈, "濕熱痢로 변증한 궤양성 대장염 환자의 芍藥湯加味方 치험 2례", 대한한방내과학회지, 27(4), pp.984~990 (2006), 대한한방내과학회
  8. 안중환, "황련해독탕이 DSS로 유발된 흰쥐의 궤양성 대장염에 미치는 영향", 대한한의학회지, 27(2), pp.182~195 (2006), 대한한의학회
  9. 김승욱, "導赤地楡湯이 궤양성 대장염 유발 흰쥐에 미치는 영향", 대한한의학회지, 23(3), pp.618~628 (2002), 대한한의학회
  10. 조남희, "특집 : 便秘에 關한 韓醫學的 考察(Special Edition : A Study on Constipation in Oriental Medicine)", 경희의학, 15(4), pp.340~355 (1999)
  11. Sánchez de Medina F, "Effect of quercitrin on acute and chronic experimental colitis in the rat", J Pharmacol Exp Ther, 278(2), pp.771~779 (1996)
  12. Rafii F, "Changes in bacterial enzymes and PCR profiles of fecal bacteria for a patient with ulcerative colitis before and after antimicrobial treatments", Dig Dis Sci, 44(3), pp.637~642 (1999)
  13. Krawisz JE, "Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assesment of inflammation in rat and hamster models", Gastroenterology, 87, pp.1344~1350 (1984)
  14. Bessy OA, "Rapid colorimetric methods for determination of alkaline phosphatase in five cubic mililiters of serum", J Biol Chem, 164, pp.321~329 (1946)
  15. Farrell RJ, "Ulcerative colitis", Lancet, 359(9303), pp.331~340 (2002)
  16. 김동현, "발효식품이 만드는 유산균이 내 몸을 살린다", pp.95~98 (2007), 헌언
  17. Weitzman SA, "Inflammation and cancer: Role of phagocyte-generated oxidants in carcinogenesis", Blood, 76, pp.655~663 (1990)
  18. 이삼범, "궤양성 대장염의 임상적 연구", 석사, pp.1991
  19. 김정룡, "김정룡 소화기계 질환", pp.258~276 (2000), 일조각
  20. 송인성, "한국인의 염증성 장질환", 대한내과학회지, 57(4), pp.661~674 (1999)
  21. 김지원, "궤양성 대장염의 발생률, 단기적 치료 반응 및 재발율의 변화 양상", 대한소화기학회지, 41, pp.15~25 (2003)
  22. 方廣, "朱震亨. 丹溪心法附餘", pp.267~284 (1982), 대성문화사
  23. 배병철, "今釋黃帝內經素問", pp.293~ (1994), 성보사
  24. 김갑성, "實用東西醫學臨床總書(一卷);", pp.632~642 (2001), 정담
  25. 武顺兴, "中药内服加保留灌肠治疗溃疡性结肠炎78例", 河南中医学院学报, 22(128), pp.68~69 (2007)
  26. 杨晨曦, "电热针结合艾灸中药治疗溃疡性结肠炎疗效观察", 中国针灸, 24(4), pp.238~240 (2004)
  27. 梁建军, "中药加超短波音频治疗溃疡性结肠炎138例疗效观察", 现代医药卫生, 20(15), pp.1452~ (2004)
  28. 宋琛虹, "中医药治疗溃疡性结肠炎临床分析", 中华中医药学刊, 25(1), pp.186~187 (2007)
  29. Gaudio E, "Dextran sulfate sodium(DSS) colitis in rats", Dig Dis Sci, 44, pp.1458~4175 (2000)
  30. Park IS, "Histological study of experimental colitis induced by dextran sulfate sodium", The Korean J Anat, 39(1), pp.27~33 (2006)
  31. Verspaget HW, "Reactive oxygen metabolites and colitis: a disturbed balance between damage and protection", Scand J Gastroenterol Suppl, 188, pp.44~51 (1991)
  32. Higgs GA, "Eicosanoids in inflammation", Ann Clin Res, 16(5-6), pp.287~299 (1984)
  33. Liang YC, "Suppression of inducible cycloxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages", Carcinogenesis, 20, pp.1945~1952 (1999)
  34. Surh YJ, "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-κB activation", Mutat Res, 480-481, pp.243~268 (2001)
  35. Baeuerle PA, "IκB-NF-κB structures: at the interface of inflammation control", Cell, 95, pp.729~731 (1998)
  36. Sartor RB, "Cytokine in intestinal inflammation: Pathophysiological and clinical considerations", Gastroenterology, 106, pp.533~539 (1994)
  37. Hill MJ, "Bacteria and aetiology of human cancer", Br J Cancer, 28, pp.94~95 (1973)
  38. Macfarlane GT, "Protein degradation by human intestinal bacteria", J Gen Microbiol, 132(6), pp.1647~1656 (1986)
  39. Rhodes JM, "Fecal mucus degrading glycosidase in ulcerative colitis and Crohn's disease", Gut, 26, pp.761~765 (1985)
  40. Tsai HH, "Increased mucin sulfatase activity in ulcerative colitis: a potential target for treatment", Gut, 36, pp.570~576 (1995)

관련 논문AI추천

궤양성 대장염 유발 생쥐에 투여한 계지가용골모려탕의 치료효과강아미, 임성우대한한방내과학회 · 대한한방내과학회지2017 · 제38권 제6호항염증조절을 통한 자금정의 DSS 유도 궤양성 대장염 완화 효과김기봉, 사복석, 사은호, 안상현, 최준용대한한방내과학회 · 대한한방내과학회지2017 · 제38권 제6호DSS으로 유발된 생쥐의 대장점막손상에 대한 導滯湯의 효과공경환, 이주아대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제3호槐花散이 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향공경환, 배광호대한한방내과학회 · 대한한방내과학회지2010 · 제31권 제3호四君子湯이 TNBS로 誘發된 생쥐의 炎症性 腸疾患에 미치는 影響김진성, 류봉하, 윤성우, 홍상선대한한방내과학회 · 대한한방내과학회지2010 · 제31권 제4호烏梅丸과 烏梅가 DSS로 유발된 생쥐의 염증성 장 질환에 미치는 영향김의수, 김태련, 신만호, 신민구, 이영수대한한방내과학회 · 대한한방내과학회지2015 · 제36권 제3호

이 간행물의 다른 논문 (19건)

丹蔘이 간성상세포의 섬유화 억제에 미치는 영향김영철, 우홍정, 최은경, 이장훈pp. 299-310한국 인삼의 경쟁력 제고를 위한 국내외 인삼 관련 특허 분석유사라, 이남헌, 손창규pp. 311-317淸燥救肺湯과 理陰煎이 호흡기 점액분비에 미치는 영향서운교, 박완열pp. 318-333解熱湯이 LPS로 자극된 대식세포에 있어 염증관련 Cytokine 발현억제에 미치는 효과이병삼, 이형구, 정희재, 김홍렬, 정승기, 김진주pp. 334-347止瀉湯의 抗瀉下作用에 關한 硏究류봉하, 김진성, 류인수pp. 348-358
원문 보기