흰쥐의 피부상처 동물모델에서 苦蔘이 신생혈관형성에 미치는 영향
Enhancement of Angiogenesis by Sophorae Radix in Full-thickness Skin Wound of Rats
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Wound healing is the restoration in injured skin tissue and one of the most important therapeutic targets. The process consists of inflammation, proliferation, and remodeling. There have been reported multiple factors that accelerate and delay wound healing. In this study we tested the hypothesis that Sophorae Radix extract would improve the recovery of wound healing after full-thickness skin wound in rats. Twenty young male Sprague-Dawley rats were used for the studies. A full-thickness skin wound was made on the dorsal skin of the rats. Either Sophorae Radix water extract (SR) or saline (Control) was orally administrated every day. The wound area was measured and the percentages of wound contraction, wound healed and wound epithelization were evaluated. After 15 days, the skin tissues were excised and examined by histopathological and immunohistochemistrical method. In results, wound area in SR group was significantly decreased to compared with Control. SR group showed the significant enhancements in the percentages of wound contraction, wound healed and wound epithelization. Histopathological examination revealed that SR induces neo-vascularization potential in wound healing process. SR treatment in rats significantly accelerated cutaneous wound healing in the angiogenesis process by increasing VEGF and TGF-β1 synthesis. These results suggest that Sophorae Radix enhance skin wound repair by increasing the angiogenic agents such as VEGF and TGF-β1.
Abstract
Wound healing is the restoration in injured skin tissue and one of the most important therapeutic targets. The process consists of inflammation, proliferation, and remodeling. There have been reported multiple factors that accelerate and delay wound healing. In this study we tested the hypothesis that Sophorae Radix extract would improve the recovery of wound healing after full-thickness skin wound in rats. Twenty young male Sprague-Dawley rats were used for the studies. A full-thickness skin wound was made on the dorsal skin of the rats. Either Sophorae Radix water extract (SR) or saline (Control) was orally administrated every day. The wound area was measured and the percentages of wound contraction, wound healed and wound epithelization were evaluated. After 15 days, the skin tissues were excised and examined by histopathological and immunohistochemistrical method. In results, wound area in SR group was significantly decreased to compared with Control. SR group showed the significant enhancements in the percentages of wound contraction, wound healed and wound epithelization. Histopathological examination revealed that SR induces neo-vascularization potential in wound healing process. SR treatment in rats significantly accelerated cutaneous wound healing in the angiogenesis process by increasing VEGF and TGF-β1 synthesis. These results suggest that Sophorae Radix enhance skin wound repair by increasing the angiogenic agents such as VEGF and TGF-β1.
참고문헌 (29)
- Takeo M, "Wound healing and skin regeneration", Cold Spring Harb Perspect Med, 5(1), pp.1~12 (2015)
- Kasuya A, "Attempts to accelerate wound healing", J Dermatol Sci, 76(3), pp.169~172 (2014)
- Zhang Z, "The effects of sodium usnic acid by topical application on skin wound healing in rats", Biomed Pharmacother, 97, pp.587~593 (2017)
- Wu H, "Ceria nanocrystals decorated mesoporous silica nanoparticle based ROS-scavenging tissue adhesive for highly efficient regenerative wound healing", Biomaterials, 151, pp.66~77 (2018)
- Asfour MH, "Chitosan based Pickering emulsion as a promising approach for topical application of rutin in a solubilized form intended for wound healing: In vitro and in vivo study", Int J Pharm, 534(1-2), pp.325~338 (2017)
- Greaves NS, "Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing", J Dermatol Sci, 72(3), pp.206~217 (2013)
- Pereira Lde P, "Modulator effect of a polysaccharide-rich extract from Caesalpinia ferrea stem barks in rat cutaneous wound healing : Role of TNF-α, IL-1β, NO, TGF-β", J Ethnopharmacol, 187, pp.213~223 (2016)
- Albaayit SF, "Effect of Clausena excavata Burm. f. (Rutaceae) leaf extract on wound healing and antioxidant activity in rats", Drug Des Devel Ther, 9, pp.3507~3518 (2015)
- Okizaki S, "Suppressed recruitment of alternatively activated macrophages reduces TGF-β1 and impairs wound healing in streptozotocin-induced diabetic mice", Biomed Pharmacother, 70, pp.317~325 (2015)
- Li WW, "The role of therapeutic angiogenesis in tissue repair and regeneration", Adv Skin Wound Care, 18(9), pp.491~500 (2005)
- Andrikopoulou E, "Current Insights into the role of HIF-1 in cutaneous wound healing", Curr Mol Med, 11(3), pp.218~235 (2011)
- Jung HA, "Re-evaluation of the antioxidant prenylated flavonoids from the roots of Sophora flavescens", Biol Pharm Bull, 31(5), pp.908~915 (2008)
- Jin JH, "Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens", J Ethnopharmacol, 127(3), pp.589~595 (2010)
- Piao XL, "Identification and characterization of antioxidants from Sophora flavescens", Biol Pharm Bull, 29(9), pp.1911~1915 (2006)
- Liu T, "Matrine inhibits proliferation and induces apoptosis of pancreatic cancer cells in vitro and in vivo", Biol Pharm Bull, 33(10), pp.1740~1745 (2010)
- Hong MH, "Sophora flavescens Aiton inhibits the production of pro-inflammatory cytokines through inhibition of the NF kappaB", IkappaB signal pathway in human mast cell line(HMC-1), 2009, pp.23(2), 251~258
- He X, "Traditional usage, phytochemistry and pharmacology of an important traditional Chinese medicine", J Ethnopharmacol, 172, pp.10~29 (2015)
- Xu X, "Enhancement of Wound Healing by the Traditional Chinese Medicine Herbal Mixture Sophora flavescens in a Rat Model of Perianal Ulceration", In Vivo, 31(4), pp.543~549 (2017)
- Gul Satar NY, "The effects of Tarantula cubensis venom on open wound healing in rats", J Wound Care, 26(2), pp.66~71 (2017)
- Yaseen Khan M, "Wound healing activity of extracts derived from Shorea robusta resin", Pharm Biol, 54(3), pp.542~548 (2016)
- Guo S, "Factors affecting wound healing", J Dent Res, 89(3), pp.219~229 (2010)
- Besson JCF, "Insulin complexed with cyclodextrins stimulates epithelialization and neovascularization of skin wound healing in rats", Injury, 48(11), pp.2417~2425 (2017)
- Ram M, "Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats", Eur J Pharmacol, 764, pp.9~21 (2015)
- Tonnesen MG, "Angiogenesis in wound healing", J Investig Dermatol Symp Proc, 5(1), pp.40~46 (2000)
- Johnson KE, "Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair", Adv Wound Care, 3(10), pp.647~661 (2014)
- Wang Y, "Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis", Pharmacol Rep, 68(6), pp.1126~1132 (2016)
- Lin LX, "Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model", Mol Med Rep, 13(2), pp.1070~1076 (2016)
- Fan ZW, "Blumea balsamifera Oil for the Acceleration of Healing of Burn Injuries", Molecules, 20(9), pp.17166~17179 (2015)
- Tamri P, "Wound healing properties of quince seed mucilage : in vivo evaluation in rabbit full-thickness wound model", Int J Surg, 12(8), pp.843~847 (2014)