3T3-L1 지방전구세포와 고지방 식이로 유도된 비만 HR-1 마우스 피부에 도포한 한약 추출 복합물의 항비만 효과 및 안전성 평가
Anti-obesity Effects and Safety of the Mixture of Herbal Extracts in 3T3-L1 Cells and HR-1 Mice Fed a High Fat Diet
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The aim of this study was to investigate whether a novel formulation of an herbal extracts has an inhibitory effect on obesity. To determine its anti-obesity effects, we performed anti-obesity-related experiments in vitro and in vivo. Thus, our present study was carried out to evaluate the anti-obesity effect of herbal extracts using a high fat diet (HFD)-induced obese mouse model and 3T3-L1 adipose cells. The effects of each herbal extracts on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. Results showed that treatment with each herbal extracts at 10~100 μg/㎖ had no effect on cell morphology and viability. Without evidence of toxicity, herbal extracts treatment decreased lipid accumulation compared with the untreated adipocytes controls as shown by the lower absorbance of Oil Red O stain. Futhermore, compared with control-differentiated mature adipocytes, each herbal extracts significantly inhibited lipid accumulation in mature 3T3-L1 adipocytes. In the HFD-fed obese mice, body weight, liver weight and white adipose tissue weights were significantly reduced by mixture of herbal extracts administration in mouse skin. Futhermore, we found that mixture of herbal extracts administration suppressed serum triglyceride (TG), and total cholesterol (TCHO) in HFD-induced obese mouse model. The mixture of herbal extracts of permeability was estimated by measuring the transepithelial electrical resistance (TEER) value in pig skin. The optimized formulations of herbal extracts (Test 3 formulation) showed skin permeation. However, test 1 formulation containing essential oil as enhancer showed maximum skin permeation. After confirming the enhanced skin permeability, in vivo studies were performed to assess whether skin irritation potential on the basis of a primary irritation index (PII) in rabbit skin. Reactions were scored for erythema/edema reactions at 24 h, 48 h and 72 h post-application. It was concluded that the test 1 formulation was not irritation (PII = 0). The present study suggests that the test 1 formulation might be of therapeutic interest with respect to the treatment of obesity.
Abstract
The aim of this study was to investigate whether a novel formulation of an herbal extracts has an inhibitory effect on obesity. To determine its anti-obesity effects, we performed anti-obesity-related experiments in vitro and in vivo. Thus, our present study was carried out to evaluate the anti-obesity effect of herbal extracts using a high fat diet (HFD)-induced obese mouse model and 3T3-L1 adipose cells. The effects of each herbal extracts on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. Results showed that treatment with each herbal extracts at 10~100 μg/㎖ had no effect on cell morphology and viability. Without evidence of toxicity, herbal extracts treatment decreased lipid accumulation compared with the untreated adipocytes controls as shown by the lower absorbance of Oil Red O stain. Futhermore, compared with control-differentiated mature adipocytes, each herbal extracts significantly inhibited lipid accumulation in mature 3T3-L1 adipocytes. In the HFD-fed obese mice, body weight, liver weight and white adipose tissue weights were significantly reduced by mixture of herbal extracts administration in mouse skin. Futhermore, we found that mixture of herbal extracts administration suppressed serum triglyceride (TG), and total cholesterol (TCHO) in HFD-induced obese mouse model. The mixture of herbal extracts of permeability was estimated by measuring the transepithelial electrical resistance (TEER) value in pig skin. The optimized formulations of herbal extracts (Test 3 formulation) showed skin permeation. However, test 1 formulation containing essential oil as enhancer showed maximum skin permeation. After confirming the enhanced skin permeability, in vivo studies were performed to assess whether skin irritation potential on the basis of a primary irritation index (PII) in rabbit skin. Reactions were scored for erythema/edema reactions at 24 h, 48 h and 72 h post-application. It was concluded that the test 1 formulation was not irritation (PII = 0). The present study suggests that the test 1 formulation might be of therapeutic interest with respect to the treatment of obesity.
참고문헌 (36)
- Despres JP, "Abdominal obesity and metabolic syndrome", Nature, 444(7121), pp.881~887 (2006)
- Sharma AM, "Adipose tissue: a mediator of cardiovascular rist", Int J Obes Relat Metab Disord, 26(4), pp.S5~S7 (2002)
- Hexsel D, "Zechmeister do Prado D. Botanical extracts used in the treatment of cellulite", Dermatol Surg, 31(7), pp.866~872 (2005)
- Wanner M, "An evidence-based assessment of treatments for cellulite", J Drugs Dematol, 7(4), pp.341~345 (2008)
- Bertin C, "A double-blind evaluation of the activity of an anti-cellulite product containing retinol, caffeine and ruscogenine by a combination of several non-invasive methods", J Cosmet Sci, 52(4), pp.199~210 (2001)
- 윤정희, "슬리밍 화장품의 특허 동향에 관한 연구", 한국미용학회지, 20(6), pp.1060~1067 (2014), 한국미용학회
- Moser K, "Passive skin penetration enhancement and its quantification in vitro", Eur J Pharm Biopharm, 52(2), pp.103~112 (2001)
- Ackermann K, "The Phenion full-thickness skin model for percutaneous absorption testing", Skin Pharmacol Physiol, 23(2), pp.105~112 (2010)
- 서지은, "피부흡수 대체시험법의 조건설정을 통한 수용성, 지용성 물질의 투과 특성 연구", 한국환경보건학회지, 43(1), pp.77~86 (2017), 한국환경보건학회
- Reichling J, "In vitro studies on release and human skin permeation of Australian tea tree oil (TTO) from topical formulations", Eur J Pharm Biopharm, 64(2), pp.222~228 (2006)
- Pappinen S, "Rat epidermal keratinocyte organotypic culture (ROC) compared to human cadaver skin: The effect of skin permeation enhancers", Eur J Pharm Sci, 30(3-4), pp.240~250 (2006)
- 정명아, "녹차씨 주정추출물의 카페인 함량 평가와 고지방식이 비만유도 C57BL", 6 마우스와 3T3-L1 지방전구세포의 항비만효과, 2016, pp.30(2), 88~94[DOI]
- Jung MA, "Anti-obesity efects on unripe Rubus coreanus Miquel extract in high fat diet-induced obese mice", Int J Biochem. Res Rev, 5(1), pp.20~26 (2015)
- Oh DR, "Antiobesity effects of unripe Rubus coreanus Miquel and its constituents: An in vitro and in vivo characterization of the underlying mechanism", Evid Based Complement Alternat Med, 2016, pp.4357656~ (2016)
- 정명아, "미숙과 복분자 열매 추출물이 고콜레스테롤 식이를 섭취시킨 C57BL", 6 Mice의 지질대사 개선 효과, 2014, pp.43(5), 650~655[DOI]
- Son Y, "Antiobesity activity of vigna nakashimae extract in high-fat diet-induced obesity", Biosci Biotechnol Biochem, 77(2), pp.332~338 (2013)
- Draize JH, "Appraisal of the safety of chemicals in food, drugs and cosmetics", pp.46~59 (1959), Assoc Food and Drug officials U.S
- Draize JH, "Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes", J Pharmcol Exp Ther, 82(3), pp.377~390 (1944)
- Kim, DH, "Molecular mechanisms underlying the link of obesity to chronic diseases", Kor J Oriental Association for Study of Obesity, 15(3), pp.121~128 (2006)
- 박미영, "Slim813의 지방산 분해 촉진을 통한 슬리밍 효능 연구", 대한화장품학회지, 38(2), pp.189~195 (2012), 사단법인 대한화장품학회[DOI]
- Vogelgesang B, "In vitro and in vivo efficacy of sulfo-carrabiose, a sugar-based cosmetic ingredient with anti-cellulite properties", Int J Cosmet Sci, 33(2), pp.120~125 (2011)
- 정현주, "비타민나무 잎 에탄올추출물의 AMPK 활성화를 통한 3T3-L1 지방전구세포의 adipogenesis 억제효과", 한국자원식물학회지, 28(5), pp.582~590 (2015), 한국자원식물학회[DOI]
- Kim GN, "Study of antiobesity effect through inhibition of pancreatic lipase activity of Diospyros kaki Fruit and Citrus unshiu Peel", Biomed Res Int, 2016, pp.1723042~ (2016)
- Pang J, "Ilex paraguariensis extract ameliorates obesity induced by high-fat diet: potential role of AMPK in the visceral adipose tissue", Arch Biochem Biophys, 476(2), pp.178~185 (2008)
- Frayn KN, "Integrative physiology of human adipose tissue", Int J Obes Realat Metab Disord, 27(8), pp.875~888 (2003)
- 최준혁, "참취(Aster scaber Thunb.) 추출물의 항산화 효과와 3T3-L1 지방전구세포에서의 지방분화 억제 효과", 한국식품과학회지, 45(3), pp.356~363 (2013), 한국식품과학회[DOI]
- Rosen ED, "Transcriptional regulation of adipogenesis", Genes Dev, 14(11), pp.1293~1307 (2000)
- Gaidhu MP, "Dysregulation of lipolysis and lipid metabolism in visceral and subcutaneous adipocytes by high-fat diet: role of ATGL, HSL, and AMPK", Am J Physiol Cell Physiol, 298(4), pp.C961~C971 (2010)
- DiGirolamo M, "Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum", Am J Physiol, 274(5), pp.1460~1467 (1998)
- Morange PE, "Influence of PAI-1 on adipose tissue growth and metabolic parameters in a murine model of diet-induced obesity", Arterioscler Thromb Vasc Biol, 20(4), pp.1150~1154 (2000)
- Levy RI, "Cholesterol, lipoproteins, apoproteins, and heart disease: present status and future prospects", Clin Chem, 27(5), pp.653~662 (1981)
- 김현주, "에너지균형에 미치는 흰지방조직 내 혈관의 역할", Annals of Pediatric Endocrinology & Metabolism, 16(3), pp.139~156 (2011), 대한소아내분비학회
- Rim JCK, "Effect of high fat and high carbohydrate diet on serum leptin and lipid concentration in rats", Korean J Nutr, 34(2), pp.123~131 (2001)
- Jayasooriya AP, "Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol enriched diets", J Ethnopharmacol, 72(1-2), pp.331~336 (2000)
- Kim HK, "Obesity and fat metabolism", J Korean Soc Study Obes, 9(1), pp.63~65 (2000)
- Wilkinson SC, "Interactions of skin thickness and physicochemical properties of test compounds in percutaneous penetration studies", Int Arch Occup Environ Health, 79(5), pp.405~413 (2006)