가미온담탕 전탕액과 발효액의 지방전구세포와 고지방식이 유도 비만쥐에 대한 항비만효과

Abstract

Objective This study aimed to determine the anti-obesity effect of Gami-ondam-tang (ODT) and fermented Gami-ondam-tang (FODT) in high-fat diet (HFD)-induced mice with obesity. Methods 3T3-L1 cells and human adipose mesenchymal stem cells (hAMSCs) used for in vitro experiments were incubated with ODT and FODT at 200 μg/mL and 400 μg/mL. The growth rate was assessed using the MTS assay. The cellular lipid content was assessed using Oil-Red-O staining. Peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer binding protein alpha (C/EBPα) were measured using real-time reverse transcriptase-polymerase chain reaction and western blotting. The anti-obesity effects of ODT and FODT were confirmed in HFD-fed mice using hematoxylin and eosin staining and serum analysis. Results ODT and FODT did not show toxicity at the indicated concentrations of 200 μg/mL and 400 μg/mL. FODT significantly inhibited the differentiation of 3T3-L1 preadipocytes and hAMSCs at concentrations of 200 μg/mL and 400 μg/mL. FODT (400 μg/mL) significantly inhibited gene and protein expressions of PPARγ. In addition, ODT (400 μg/mL) and FODT (200 μg/mL, 400 μg/mL) reduced the expression of C/EBPα. ODT and FODT inhibited lipid accumulation in the fatty liver and epididymal white adipose tissue of HFD-induced mice. Moreover, FODT reduced body weight gain, total cholesterol, and LDL cholesterol levels in mice. Conclusions Based on these results, ODT and FODT have anti-obesity effects. Therefore, ODT and FODT may be clinically useful for the treatment of obesity. Furthermore, FODT showed better anti-obesity effects than ODT.