Lactiplantibacillus plantarum으로 발효한 황련해독탕 가미방의 기능성 화장품 조성물로서의 생리활성 증대에 관한 연구
A study on the increase of Physiological Activity as a Functional Cosmetic Composition of Hwangryunhaedoktang-Gamibang Fermented with Lactiplantibacillus Plantarum
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Hwangryunhaedoktang is one of the prescriptions used in traditional medicine for skin diseases. In this study, Hwangryunhaedoktang-Gamibang (HG) was fermented with Lactiplantibacillus plantarum, a probiotic lactic acid bacterium, to evaluate its potential as a functional cosmetic composition. Strains with anti-inflammatory activity were selected by isolating lactic acid bacteria from kimchi, a traditional Korean fermented food. HG was inoculated with lactic acid bacteria and the viability was measured. The supernatant was obtained by centrifugation of fermented Hwangryunhaedoktang-Gamibang (HGF) and HG, and the filtered supernatant was freeze-dried and used in the experiment. By measuring DPPH and ABTS scavenging activity, it was confirmed that the antioxidant activity was increased. RAW264.7 cells were inoculated with HG and HGF to confirm anti-inflammatory activity through NO assay, and production levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured through ELISA assay. It was confirmed that HGF had a greater decrease in production than HG. Through lactobacilli fermentation, the beneficial probiotic properties and antioxidant and pro-inflammatory activities of lactic acid bacteria suggest potential clinical or technical applications.
Abstract
Hwangryunhaedoktang is one of the prescriptions used in traditional medicine for skin diseases. In this study, Hwangryunhaedoktang-Gamibang (HG) was fermented with Lactiplantibacillus plantarum, a probiotic lactic acid bacterium, to evaluate its potential as a functional cosmetic composition. Strains with anti-inflammatory activity were selected by isolating lactic acid bacteria from kimchi, a traditional Korean fermented food. HG was inoculated with lactic acid bacteria and the viability was measured. The supernatant was obtained by centrifugation of fermented Hwangryunhaedoktang-Gamibang (HGF) and HG, and the filtered supernatant was freeze-dried and used in the experiment. By measuring DPPH and ABTS scavenging activity, it was confirmed that the antioxidant activity was increased. RAW264.7 cells were inoculated with HG and HGF to confirm anti-inflammatory activity through NO assay, and production levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured through ELISA assay. It was confirmed that HGF had a greater decrease in production than HG. Through lactobacilli fermentation, the beneficial probiotic properties and antioxidant and pro-inflammatory activities of lactic acid bacteria suggest potential clinical or technical applications.
참고문헌 (33)
- Chan IL, "Characteristics and management of Asian skin", Int J Dermatol, 58(2), pp.131~143 (2019)
- Kanwar IL, "Models for acne : A comprehensive study", Drug Discov Ther, 12(6), pp.329~340 (2018)
- Fiedler F, "Acne and Nutrition : A Systematic Review", Acta Derm Venereol, 97(1), pp.7~9 (2017)
- 이지혜, "黃連解毒湯 열수 추출물의 급성독성 연구", 대한한의학 방제학회지, 18(2), pp.159~166 (2010), 대한한의학방제학회
- 기호필, "黃連解毒湯의 아토피 피부염 개선 效果", 대한한의학 방제학회지, 21(1), pp.80~90 (2013), 대한한의학방제학회
- 홍철희, "황련해독탕 약침치료를 병행한 지루성피부염 치험 2례", 한방안이비인후피부과학회지, 25(2), pp.68~75 (2012), 대한한방안이비인후피부과학회[DOI]
- Yu Y, "Changing our microbiome : probiotics in dermatology", Br J Dermatol, 182(1), pp.39~46 (2020)
- Shokryazdan P, "Probiotics : From Isolation to Application", J Am Coll Nutr, 36(8), pp.666~676 (2017)
- Sniffen JC, "Choosing an appropriate probiotic product for your patient : An evidence-based practical guide", PLoS One, 13(12), pp.e0209205~ (2018)
- Azad MAK, "Immuno- modulatory Effects of Probiotics on Cytokine Profiles", Biomed Res Int, pp.8063647~ (2018)
- Molska M, "Potential Mechanisms of Probiotics Action in the Prevention and Treatment of Colorectal Cancer", Nutrients, 11(10), pp.2453~ (2019)
- Valdes-Varela L, "Probiotics for Prevention and Treatment of Clostridium difficile Infection", Adv Exp Med Biol, 1050, pp.161~176 (2018)
- 임수진, "계분으로부터 Lactobacillus salivarius의 분리 및 생균제적 특성", 한국미생물·생명공학회지, 35(3), pp.98~103 (2007), 한국미생물·생명공학회
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol. Med, 26, pp.1231~1237 (1999)
- Leong, LP, "An investigation of antioxidant capacity of fruits in Singapore markets", Food Chemistry, 76(1), pp.69~75 (2002)
- Mottin VHM, "An approach on the potential use of probiotics in the treatment of skin conditions : acne and atopic dermatitis", Int J Dermatol, 57(12), pp.1425~1432 (2018)
- Platsidaki E, "Dessinioti C. Recent advances in understanding Propionibacterium acnes (Cutibacterium acnes) in acne", F1000 Faculty Rev, 7, pp.1953~ (2018)
- Xu H, "Acne, the Skin Microbiome, and Antibiotic Treatment", Am J Clin Dermatol, 20(3), pp.335~344 (2019)
- Dessinioti C, "Acne treatments : future trajectories", Clin Exp Dermatol, 45(8), pp.955~961 (2020)
- Soliman YS, "Update on acne scar treatment", Cutis, 102(1), pp.21~25 (2018)
- Clark AK, "Edible Plants and Their Influence on the Gut Microbiome and Acne", Int J Mol Sci, 18(5), pp.1070~ (2017)
- Kwon HH, "Comparison of clinical and histological effects between lactobacillus-fermented Chamaecyparis obtusa and tea tree oil for the treatment of acne : an eight-week double-blind randomized controlled split-face study", Dermatology, 229(2), pp.102~109 (2014)
- Islam SU, "Clinical Uses of Probiotics", Medicine (Baltimore), 95(5), pp.e2658~ (2016)
- Suez J, "The pros, cons, and many unknowns of probiotics", Nat Med, 25(5), pp.716~729 (2019)
- 우배언, "金銀花 열수 추출물이 피부 美白에 미치는 영향", 한방안이비인후피부과학회지, 30(2), pp.38~50 (2017), 대한한방안이비인후피부과학회[DOI]
- 김미진, "연교를 함유한 처방단 추출물들의 항염증 효과", 대한화장품학회지, 35(4), pp.277~285 (2009), 사단법인 대한화장품학회
- Batiha GE, "Syzygium aromaticum L. (Myrtaceae): Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities", Biomolecules, 10(2), pp.202~ (2020)
- Wang GW, "Illicium verum : a review on its botany, traditional use, chemistry and pharmacology", J Ethnopharmacol, 136(1), pp.10~20 (2011)
- Chung Y, "Critical regulation of early Th17 cell differentiation by interleukin-1 signaling", Immunity, 30, pp.576~587 (2009)
- Toshio T, "IL-6 in Inflammation, Immunity, and Disease", Cold Spring Harb PersPect. Biol, 6, pp.a016295~ (2014)
- Felson DT, "Preliminary definition of improvement in rheumatoid arthritis", ArthritisRheum, 38, pp.727~735 (1995)
- Moller B, "Inhibition of IL-1, IL-6, and TNF-alpha in immune-mediated inflammatory diseases", Springer Semin Immunopathol, 27(4), pp.391~408 (2006)
- 이동은, "Clinical Evidence of Effects of Lactobacillus plantarum HY7714 on Skin Aging: A Randomized, Double Blind, Placebo-Controlled Study", Journal of Microbiology and Biotechnology, 25(12), pp.2160~2168 (2015), 한국미생물·생명공학회[DOI]