심근세포에서 苦蔘 유기용매 추출물의 항독성 효과
Cytoprotective Effect of Organic Solvents Extracts of Sophorae Radix in H9c2 Cells
1
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
To test the cytoprotective effect of sophorae radix (SR) against hydrogen peroxide (H2O2)-induced cytotoxicity, we investigated the cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in the presence of methylene chloride, n-butanol, ethyl acetate and water soluble fraction of SR water extracts in H9c2 cells. These results were obtained as followed ; H2O2 decreased the cell viability of H9c2 cells in a dose dependent manner. Cells pretreated with SR water extracts were protected the H2O2-induced decrease of viability in H9c2 cells. Among organic solvents fractions of SR water extracts, ethyl acetate soluble fractions of SR protected the decrease of viability induced by H2O2 in H9c2 cells. These results suggest that ethyl acetate soluble fractions of SR water extracts is effective in the prevention of H2O2-induced cytotoxicity.
참고문헌 (16)
- "急性附子草烏中毒에서 甘豆湯과 苦蔘의 應用", pp.399~405 (1993)
- "中藥藥理毒理與臨床", pp.63~64 (1992)
- "重修政和經史證類備急本草", pp.198~ (1982), 人民衛生出版社
- "本草備要解析", pp.186~187 (1985), 國興出版社
- "약초의 성분과 이용", pp.342~344 (1991), 서울 일월서각
- "中華人民共和國藥典", pp.174~ (1985), 人民衛生出版社
- "新編中藥大辭典", pp.1368~1370 (1982)
- "漢藥臨床應用", pp.137~138 (1990), 서울
- "동의학연구소", pp.127~128 (1994), 서울 여강출판사
- "陳無擇三因方", pp.22~ (1978), 臺聯國風出版社
- "苦蔘 煎湯液이 培養心筋細胞에 미치는 影響", 20(1), pp.142~150 (1999)
- "苦蔘 전탕액 분획물이 배양 심근세포에 미치는 영향", 15(1), pp.149~155 (2000)
- "Properties of a clonal muscle cell line from rat heart", pp.367~81 (1976)
- "한국산 생약으로부터 항암물질의 개발 Ethyl Acetate 가용성 분획의 인체 구강유상피암종세포에 미치는 독성작용", 29(1), pp.22~27 (1998)
- "radical scavenging activity of flavonoids obtained from some medicinal plants", (hara t), pp.1 1202~52001
- "Bjo가 J Free radicals as mediators of tissue injury", pp.43~57 (1980)