苦蔘이 H2O2에 의한 대동맥 평활근세포 고사 및 전체 단백질 발현에 미치는 영향
Study of Sophorae Radix on H2O2-mediated Apoptosis and Total Protein Expression Pattern in Vascular Smooth Muscle Cells
1
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Apoptosis of vascular smooth muscle cells(VSMCs) is essential in atherogenesis, being a factor that modulates its early progression rather than a terminal event in the course of the disease. Various stimuli, including oxide lipoproteins, altered hemodynamic stress and free radical, can induced VSMCs apoptosis in vitro. The protective effects of Sophorae Radix (SR) on apoptotic cell death induced by H2O2 were investigated in VSMCs. The viability of VSMCs was markedly decreased by H2O2. Sophorae Radix protected the H2O2-induced apoptotic death of VSMCs, which was characterized as nuclear fragmentation and increase of sub-G0/G1 fraction.. Sophorae Radix decreased the activation of caspase-3 like protease induced by H2O2 and recovered control level from H2O2-induced PARP, Bak, Bcl-XL and mitochondrial membrane potential. These results suggest that Sophorae Radix protected VSMCs apoptotic death induced by H2O2 via inactivation of caspase-3 and modulation of mitochondrial function. Also, the expression profile of proteins by using two-dimensional (2-D) gel electrophoresis was screened. Future investigations will need to explore the use of an antiatherosclerotic therapy of Sophorae Radix, which relies on inhibition of the proapoptotic activation of the vascular smooth muscle cells.
참고문헌 (31)
- "東醫心系內科學(上),", pp.437~444 (1995)
- "The decline of ischemic heart disease mortality rates Analysis of the comparative efforts of medical intervention and changes in life style Annals of Internal Medicine" (1984)
- "The pathogenesis of atherosclerosis a perspective for the 1990s", pp.801~9 (1993)
- "急性附子草烏中毒에서 甘豆湯과 苦蔘의 應用", pp.399~405 (1993)
- "苦蔘煎湯液에 의한 循環器 Anaphylaxis의 抑制 效果" (1997), 원광대학교 대학원
- "苦蔘煎湯液이 培養心筋細胞에 미치는 影響", pp.142~150 (1999)
- "徐在鈴 外 苦蔘 煎湯液 分劃物이 培養 心筋細胞에 미치는 影響", 15(1), pp.149~155 (2000)
- "Apoptosis of human vascular smooth muscle cells derived from normal", pp.2266~2274 (1995)
- "High glucose-triggered apoptosis in", pp.1323~1327 (1995)
- "Oxidazed low density lipoprotein induces apoptosis of human endothelial cells by activation of CPP-32 like protease A mechanistic clue to the response to injury hypothesis", pp.1760~1763 (1992)
- "Induction of heat shock response leads to apoptosis in endothelial cells previously exposed to endotoxin", pp.165~170 (1993)
- "Mitochondrial control of cell death", pp.513~519 (2000)
- "Controlling the mitochondrial", pp.52~60 (2000)
- "약초의 성분과 이용", pp.342~344 (1991)
- "The pharmacology of Chinese herbs", pp.63~67 (1993), CRC press
- "Chinese Drugs of plant Origin", pp.931~943 (1992), Springer-Verlag
- "NGF exhibits a pro- apoptotic activity for human vascular smooth", pp.243~246 (1997)
- "Reactive oxygen species induce", pp.249~252 (1997)
- "Fas is expressed in human atherosclerotic intima and promotes", pp.2200~2208 (1997)
- "Atherosclerosis-an inflammatory disease,", pp.115~126 (1999)
- "Shear stress inhibits", pp.71~74 (1996)
- "Apoptosis in the vascular", pp.11~22 (2001)
- "Identification and inhibition of the ICE", CED-3 protease, 1995, 37~43
- "a family of highly conserved aspartate specific cysteine proteases", pp.33~42 (1997)
- "An induced proximity model for caspase-8 activation", pp.2926~2930 (1998)
- "Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC", pp.483~487 (1999)
- "arbiters of cell survival", pp.1322~1326 (1998)
- "Bcl-2 and the regulation of programmed cell death", pp.1~6 (1994)
- "Proteomics to study genes and genomes", pp.837~46 (2000)
- "From proteins to proteomes large scale protein identification by two-dimensional electrophoresis and amino acid analysis", pp.61~65 (1996)
- "Twenty years of two-dimensional electrophoresis", pp.443~453 (1996)